FastQCFastQC Report
Thu 26 May 2016
ERR580225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5832907
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150300.041.357151
GGTATCA84950.039.8061261
CAACGCA167100.035.3531045
ATCAACG175700.033.6229633
TATCAAC188100.032.804512
AACGCAG208200.030.3369856
GTGGTAT28650.029.301481
ACGCAGA211400.027.8992567
CGCAGAG216150.027.0252518
TCAACGC232050.025.8631734
CTGTGCG61650.025.6892079
TAGTACT62000.025.6210844
TACCCCG5350.025.4749285
AAGCTAA41200.025.0945459
GTACTAG46050.024.9893151
CGTTAAC22000.024.8673481
GCAGAGT238150.024.2721569
GGGCACC55550.024.19598494
GTACATG287850.023.0527111
CTAGTAC71600.022.9078773
Produced by FastQC (version 0.11.5)