FastQCFastQC Report
Thu 26 May 2016
ERR580224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270106
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264810.3202014581191583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214430.25928325465235874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122520.14814804066598422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124400.050.0776331
GTATCAA228200.042.2981761
ATCAACG277950.033.94693
CAACGCA278800.033.8240855
TATCAAC291100.032.8031852
TCAACGC295300.032.0149084
AACGCAG301000.031.9288526
ACGCAGA336150.028.03017
CGCAGAG344250.027.2470478
GTGGTAT53050.026.5879081
GTACATG281900.025.1342811
GTACTAG32150.024.2759041
GCAGAGT380250.024.1777889
TACATGG288050.023.8957962
TGGTATC60300.023.3911882
ACATGGG287500.022.5826663
CATGGGG221000.020.9317934
GAGTACT232450.020.85033412-13
AGAGTAC352500.020.36854610-11
CAGAGTA372350.020.12217310-11
Produced by FastQC (version 0.11.5)