FastQCFastQC Report
Thu 26 May 2016
ERR580224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270106
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237700.28742074164466574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197880.23927141925387654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118040.14273093960343433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131350.051.918731
GTATCAA237650.041.1383971
ATCAACG287000.033.1288033
CAACGCA287700.033.0623325
TATCAAC303100.031.9732362
TCAACGC306400.031.1991984
AACGCAG308150.031.1875216
GTGGTAT53650.028.2176721
ACGCAGA346350.027.422117
CGCAGAG355800.026.548498
GTACATG280250.024.249581
TGGTATC59500.024.012672
GCAGAGT396150.023.500229
TACATGG283550.023.486812
GTACTAG34900.023.3779561
ACATGGG283650.022.6504843
GAGTACT238100.020.92178712-13
CTAACGC15150.020.7851873
CATGGGG221800.020.7232824
AGAGTAC362350.020.32917810-11
Produced by FastQC (version 0.11.5)