FastQCFastQC Report
Thu 26 May 2016
ERR580223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14812694
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419820.2834190728573749No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331120.2235380005824734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191420.12922699949111216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217750.054.1570051
GTATCAA408100.042.088971
CAACGCA480000.034.6335955
ATCAACG485950.034.241343
TATCAAC515750.032.9669532
AACGCAG524150.032.2122736
TCAACGC523800.031.9009364
ACGCAGA577700.028.7452647
CGCAGAG595800.027.8317248
GTGGTAT85150.025.3462221
GTACATG528400.025.129691
GCAGAGT657800.024.8422349
TACATGG536250.024.2359682
GTACTAG68400.023.7163721
ACATGGG533250.023.3479583
GAGTACT367350.022.05009812-13
CAGAGTA647250.021.12138210-11
CATGGGG404150.020.9634954
AGAGTAC605800.020.78201710-11
GTACTTT404450.019.83355114-15
Produced by FastQC (version 0.11.5)