FastQCFastQC Report
Thu 26 May 2016
ERR580223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14812694
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373920.25243213692256117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309470.20892215825156452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182620.12328614902866421No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216350.053.538551
GTATCAA412250.042.386031
CAACGCA488350.034.713185
ATCAACG490850.034.5665053
TATCAAC517550.033.3994182
AACGCAG531400.032.3324936
TCAACGC531350.032.1170234
ACGCAGA588800.028.7255677
CGCAGAG604850.027.8700838
GTACTAG67450.025.032041
GCAGAGT670700.024.9026249
GTGGTAT94050.024.872421
GTACATG538900.023.7255611
TACATGG544300.023.0715922
ACATGGG545050.021.9973933
GAGTACT372700.021.49841312-13
CAGAGTA659350.020.77235610-11
TGGTATC106900.020.7072622
AGAGTAC619400.020.69716510-11
CATGGGG403550.019.7639734
Produced by FastQC (version 0.11.5)