FastQCFastQC Report
Thu 26 May 2016
ERR580222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8115565
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214620.2644547853415012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161750.19930836608418515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104510.12877723239232267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111550.047.2635651
GTATCAA198700.040.5698551
CAACGCA224400.034.116815
ATCAACG232550.032.966473
TATCAAC249850.031.8699592
AACGCAG255250.030.5295226
TCAACGC254950.030.2906514
GTACATG274200.028.0955091
GTACTAG48550.027.8993281
ACGCAGA276550.027.753077
CTAACGC22050.027.302513
TAACGCC22050.027.088544
CGCAGAG284850.026.9443998
TACATGG279550.026.8856872
ACATGGG274650.026.5603263
CTGTGCG68100.025.5355459
TCTAACG23950.025.1367032
TAGTACT70350.025.0033384
CATGGGG220650.024.7467844
GTGGTAT45850.024.2082351
Produced by FastQC (version 0.11.5)