FastQCFastQC Report
Thu 26 May 2016
ERR580222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8115565
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188520.23229436274615506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150050.18489162492075412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102970.1268796442391873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112500.045.923461
GTATCAA201550.040.7240451
CAACGCA227750.034.400945
ATCAACG234550.033.4038053
TATCAAC257150.031.5660572
AACGCAG258350.031.0896636
TCAACGC258200.030.580614
CTAACGC20750.029.4456423
TCTAACG21150.029.1122282
GTACTAG50050.028.8352491
TAACGCC21300.028.6851314
GTACATG272650.027.9490431
ACGCAGA282300.027.8858387
TACATGG277700.026.9790692
CGCAGAG293100.026.8748448
ACATGGG275200.026.2153553
TAGTACT70600.025.7631744
CTGTGCG69450.025.648239
GTGGTAT44250.024.6470051
CATGGGG218250.024.506494
Produced by FastQC (version 0.11.5)