FastQCFastQC Report
Thu 26 May 2016
ERR580221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217068
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61590.19144761627668425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48110.14954610844408636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.047.2631951
GTATCAA106700.040.76421
CAACGCA119250.034.7666665
ATCAACG123350.033.648653
TATCAAC132550.032.415532
AACGCAG135550.031.5050896
GTGGTAT20100.030.6462141
GTACTAG25000.030.2822021
TCAACGC145850.028.8780564
CGTTAAC8200.028.6719861
ACGCAGA147650.028.0017937
CGCAGAG148850.027.7440538
TACCCCG2650.026.6071435
GTGATCG17500.024.9674828
GCATCGT26250.024.8779938
GCAGAGT161950.024.865899
TATTCTC17450.023.7050085
GTATAAG26300.023.4216331
TGGTATC24900.023.4104082
GTACATG185400.023.2574021
Produced by FastQC (version 0.11.5)