FastQCFastQC Report
Thu 26 May 2016
ERR580221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217068
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53110.16508821075588082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.14289408865463832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.051.0341451
GTATCAA105550.039.3319471
TACCCCG2200.034.1804855
CAACGCA121850.032.9006425
ATCAACG126350.031.7675573
TATCAAC134750.030.6004122
AACGCAG142350.028.9527156
GTACTAG25650.028.7776451
TCAACGC146150.027.5602514
GTGGTAT18950.027.5395451
TATTCTC15550.027.503755
ACGCAGA148200.026.9219577
GCTATTG31750.026.6433898
CGCAGAG149750.026.5185978
GGGCTAT34850.026.0256566
AGGGCTA33050.025.8809875
CTATTGA33050.025.4520029
GGCTATT35450.025.3200327
ATTTAGG34750.025.0299431
GCAGAGT164600.023.9536939
Produced by FastQC (version 0.11.5)