##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580221_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3217068
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55020161215119	34.0	31.0	34.0	31.0	34.0
2	32.67273119498873	34.0	31.0	34.0	31.0	34.0
3	32.72356661407219	34.0	31.0	34.0	31.0	34.0
4	36.233927601157326	37.0	35.0	37.0	35.0	37.0
5	36.179979409822856	37.0	35.0	37.0	35.0	37.0
6	36.21437812318546	37.0	37.0	37.0	35.0	37.0
7	36.16739932136964	37.0	36.0	37.0	35.0	37.0
8	36.16208796332561	37.0	37.0	37.0	35.0	37.0
9	37.863193441978844	39.0	38.0	39.0	35.0	39.0
10-11	37.82435683672213	39.0	38.0	39.0	35.0	39.0
12-13	37.781773030598046	39.0	38.0	39.0	35.0	39.0
14-15	39.15076600805454	41.0	39.0	41.0	36.0	41.0
16-17	39.14426536834161	40.0	38.5	41.0	36.0	41.0
18-19	39.13150794450102	40.0	38.5	41.0	35.5	41.0
20-21	39.10046461560651	40.0	39.0	41.0	35.0	41.0
22-23	39.02616963645157	40.0	38.5	41.0	35.0	41.0
24-25	38.947801538543786	40.0	38.0	41.0	35.0	41.0
26-27	38.814482006597316	40.0	38.0	41.0	35.0	41.0
28-29	38.727914517193916	40.0	38.0	41.0	35.0	41.0
30-31	38.59347377798666	40.0	38.0	41.0	34.5	41.0
32-33	38.508740101235034	40.0	38.0	41.0	34.0	41.0
34-35	38.42920541312773	40.0	38.0	41.0	34.0	41.0
36-37	38.32037137542632	40.0	38.0	41.0	34.0	41.0
38-39	38.20903443756862	40.0	38.0	41.0	34.0	41.0
40-41	38.1064963500927	40.0	37.0	41.0	33.5	41.0
42-43	38.117564191990965	40.0	37.0	41.0	33.5	41.0
44-45	38.08584804548738	40.0	37.0	41.0	33.5	41.0
46-47	37.914311571903355	40.0	37.0	41.0	33.0	41.0
48-49	37.72339953647234	40.0	37.0	41.0	33.0	41.0
50-51	37.52704481223275	40.0	36.0	41.0	33.0	41.0
52-53	37.348417565311024	39.5	36.0	41.0	32.5	41.0
54-55	37.12569706328868	39.0	35.0	41.0	32.0	41.0
56-57	36.868045686320585	39.0	35.0	41.0	32.0	41.0
58-59	36.610053315627766	38.5	35.0	40.5	31.0	41.0
60-61	36.34429704314611	38.0	35.0	40.0	31.0	41.0
62-63	36.03137934914649	37.0	35.0	40.0	31.0	41.0
64-65	35.735674999720246	37.0	35.0	40.0	30.5	41.0
66-67	35.42164573456327	36.0	34.5	39.0	30.0	41.0
68-69	35.118858693692516	36.0	34.0	39.0	30.0	41.0
70-71	34.82641150264775	35.0	34.0	38.5	29.5	40.5
72-73	34.53521669420727	35.0	34.0	37.5	29.0	40.0
74-75	34.20782961379741	35.0	34.0	37.0	29.0	39.0
76-77	32.70438765981943	34.0	31.5	35.5	27.0	38.5
78-79	33.60953498651567	35.0	33.5	36.0	29.0	39.0
80-81	33.615286807739224	35.0	34.0	36.0	29.0	37.5
82-83	33.41387344003919	35.0	33.5	35.5	29.0	37.0
84-85	33.223989670097126	35.0	33.0	35.0	29.0	37.0
86-87	33.06343835442708	35.0	33.0	35.0	29.0	36.0
88-89	32.91303183519901	35.0	33.0	35.0	29.0	36.0
90-91	32.767445077318854	35.0	33.0	35.0	28.5	36.0
92-93	32.63086465688633	35.0	33.0	35.0	27.0	35.5
94-95	32.52132967037066	35.0	33.0	35.0	27.0	35.0
96-97	32.411271692112194	35.0	33.0	35.0	27.0	35.0
98-99	32.33167157175416	35.0	33.0	35.0	27.0	35.0
100	30.42999899287177	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09687542464555321
1101	2	-0.07651432203493869
1101	3	-0.042213115130536494
1101	4	-0.022950615480297643
1101	5	-0.06441167899227906
1101	6	-0.015358861276581592
1101	7	-0.05187941012763986
1101	8	-0.012344010068503053
1101	9	-0.017287132047066223
1101	10-11	-0.056862808886073424
1101	12-13	-0.09331201549596813
1101	14-15	-0.08576753320901531
1101	16-17	-0.10501365175259281
1101	18-19	-0.07804635571432073
1101	20-21	-0.085186631666744
1101	22-23	-0.08638479501877327
1101	24-25	-0.10667065517105101
1101	26-27	-0.0956989823967902
1101	28-29	-0.04514333250082103
1101	30-31	-0.16100516212098626
1101	32-33	-0.12354499302876576
1101	34-35	-0.16426850004366145
1101	36-37	-0.10595978592561295
1101	38-39	-0.17207498682827094
1101	40-41	-0.17763745705896383
1101	42-43	-0.11186096666433087
1101	44-45	-0.08836883576747567
1101	46-47	-0.07118084450137019
1101	48-49	-0.059571034443955284
1101	50-51	0.03514937762462722
1101	52-53	-0.0015842420801135404
1101	54-55	-0.09510181911220172
1101	56-57	-0.0908477959192382
1101	58-59	-0.023739093031167613
1101	60-61	-0.09532752945710143
1101	62-63	-0.09709853335357366
1101	64-65	0.03249568258377877
1101	66-67	0.019174905749274274
1101	68-69	0.015419942859367097
1101	70-71	-0.005064081395836695
1101	72-73	-0.06318214912405296
1101	74-75	0.037661297237910674
1101	76-77	-0.01649869720152708
1101	78-79	0.01879113104420327
1101	80-81	-0.019863414423014092
1101	82-83	0.03758754730663583
1101	84-85	-0.09076810814798364
1101	86-87	-0.10964805609101802
1101	88-89	-0.08689944679054662
1101	90-91	-0.07613892205456096
1101	92-93	-0.16191596602931924
1101	94-95	-0.11530678354936441
1101	96-97	-0.18489619807183288
1101	98-99	-0.14953778481928026
1101	100	-0.1700125809237214
1102	1	-0.0925648419430729
1102	2	-0.12741290996818577
1102	3	-0.08600617996723514
1102	4	-0.02009029051698974
1102	5	-0.10259826695751428
1102	6	-0.028524554173053218
1102	7	-0.04304189578103035
1102	8	-0.017867706476145884
1102	9	-0.07355285333684236
1102	10-11	0.04318687938749832
1102	12-13	0.013544666327121035
1102	14-15	0.024824067173291553
1102	16-17	0.03647545681778297
1102	18-19	-0.00807376924472436
1102	20-21	-0.03157158481780442
1102	22-23	0.002372558245852474
1102	24-25	-0.08199087959808793
1102	26-27	-0.04054349909085175
1102	28-29	-0.08044670090588113
1102	30-31	-0.0486685735558936
1102	32-33	-0.053785656232243184
1102	34-35	-0.042875948685782816
1102	36-37	-0.08492025125578806
1102	38-39	-0.16702167940882617
1102	40-41	-0.1130802925497818
1102	42-43	-0.025906351953402407
1102	44-45	0.017261535464918154
1102	46-47	-0.013229021131692775
1102	48-49	0.050110980600500454
1102	50-51	-0.007471463856781213
1102	52-53	-0.06570181033400502
1102	54-55	-0.09791775455143892
1102	56-57	-0.053434725403882055
1102	58-59	-0.05738555246674082
1102	60-61	-0.10538355623479134
1102	62-63	-0.04019046365802126
1102	64-65	-0.03928937614237071
1102	66-67	-0.0578291213003439
1102	68-69	-0.18919686757336507
1102	70-71	-0.1956894937985183
1102	72-73	-0.23344306754730582
1102	74-75	-0.14280551259496832
1102	76-77	-0.21946079814570396
1102	78-79	-0.12485406868866278
1102	80-81	-0.10499212404754132
1102	82-83	-0.13117942368820934
1102	84-85	-0.17488115746834154
1102	86-87	-0.17864117371525623
1102	88-89	-0.11642957767966067
1102	90-91	-0.06958916779657187
1102	92-93	-0.15822653673463094
1102	94-95	-0.14158718137547766
1102	96-97	-0.2194451445204706
1102	98-99	-0.22062820747952827
1102	100	-0.052326154920258716
1103	1	0.017570217222662166
1103	2	0.020713151184970968
1103	3	0.002121401885638363
1103	4	0.02608094350656387
1103	5	-0.04543977526514453
1103	6	0.04395660492367881
1103	7	0.017880732149855305
1103	8	-0.062229469614223376
1103	9	-0.041264070843006095
1103	10-11	0.006263703842932955
1103	12-13	0.009044750046491856
1103	14-15	-0.02022595414076278
1103	16-17	0.009506191129702302
1103	18-19	0.03289240489318246
1103	20-21	0.0023340533381670525
1103	22-23	-0.006384912798161224
1103	24-25	-0.03722851723961185
1103	26-27	-0.014889072439132178
1103	28-29	-0.05714976325538146
1103	30-31	-0.0653158877009048
1103	32-33	-0.050158423412312914
1103	34-35	-0.00942217269940926
1103	36-37	-0.11661440376094134
1103	38-39	-0.08539377440725815
1103	40-41	-0.08955555327294462
1103	42-43	-0.03173506049916597
1103	44-45	-0.003067408870194299
1103	46-47	0.01986409422389812
1103	48-49	-0.04884663232156328
1103	50-51	-0.07342908405879456
1103	52-53	-0.034814518449444165
1103	54-55	7.873858180431625E-4
1103	56-57	0.020461675310194494
1103	58-59	0.03137518241948811
1103	60-61	-0.041868054399834875
1103	62-63	-0.06771846515930235
1103	64-65	-0.03082987730999065
1103	66-67	-0.08314917601317973
1103	68-69	-0.049026876802720665
1103	70-71	-0.0936347696396922
1103	72-73	-0.10224014958339467
1103	74-75	-0.06640915839165729
1103	76-77	-0.028083772197554424
1103	78-79	0.024698356220724804
1103	80-81	-0.05828864512339038
1103	82-83	-0.04170565858940023
1103	84-85	0.058903876258021626
1103	86-87	0.0803250902086532
1103	88-89	0.11054061822367345
1103	90-91	0.0022061597726619198
1103	92-93	-0.040289903806467464
1103	94-95	-0.012191813200324475
1103	96-97	-0.05387340187139955
1103	98-99	-0.02423995019331926
1103	100	0.1947784489981288
1104	1	-0.06636837303088328
1104	2	-0.04363846426825546
1104	3	-0.035741993032758046
1104	4	-0.031419937992801295
1104	5	-0.07487065097432577
1104	6	-0.03662914304965881
1104	7	0.02001421611443277
1104	8	0.024970176921236487
1104	9	0.06987226340110908
1104	10-11	-0.010825394614158768
1104	12-13	-0.015874558858008925
1104	14-15	-0.060726673294873024
1104	16-17	-0.09685235760591127
1104	18-19	-0.06877139812895194
1104	20-21	-0.013730658345693314
1104	22-23	-0.07507199172744095
1104	24-25	-0.060856925851219046
1104	26-27	-0.12380632785303192
1104	28-29	-0.10167304800699384
1104	30-31	-0.1574972340344445
1104	32-33	-0.18263804868959
1104	34-35	-0.04759551733794609
1104	36-37	-0.05953009704397516
1104	38-39	-0.10760814797666285
1104	40-41	3.8194969978633253E-4
1104	42-43	0.06157493914312795
1104	44-45	-0.021142568041263132
1104	46-47	-9.965522036736729E-5
1104	48-49	0.041698780913932865
1104	50-51	-0.004537599480222809
1104	52-53	-0.02282819082885368
1104	54-55	-0.03767989021331175
1104	56-57	-0.07553635689233573
1104	58-59	-0.08056196991893927
1104	60-61	-0.1273099106232607
1104	62-63	-0.012040921360480183
1104	64-65	-0.03174134961636099
1104	66-67	-0.024728076004180366
1104	68-69	-0.08562511867828704
1104	70-71	0.016401803248022873
1104	72-73	-0.04637235129560224
1104	74-75	-0.042044103266988486
1104	76-77	-0.09471215211375039
1104	78-79	-0.09848032065364976
1104	80-81	0.010826899493842745
1104	82-83	0.01475963313480122
1104	84-85	0.0400823416511642
1104	86-87	-0.022520629517394752
1104	88-89	-0.025359460433186598
1104	90-91	-0.054697621378068106
1104	92-93	-0.004276085198398505
1104	94-95	-0.08313492121181554
1104	96-97	-0.23607224454233489
1104	98-99	-0.2246332541787197
1104	100	-0.04740057811745757
1105	1	0.0036626311016974
1105	2	0.003710959567065686
1105	3	0.035262623623900424
1105	4	0.060893480917457055
1105	5	0.03653947100441002
1105	6	0.012434057441915058
1105	7	0.05347677887808544
1105	8	0.04310107510416117
1105	9	0.025583824249551412
1105	10-11	0.018492580833942895
1105	12-13	0.04191192096666185
1105	14-15	0.004809278147860141
1105	16-17	-0.03505811933083436
1105	18-19	-0.024596950499862658
1105	20-21	-0.008119210942240329
1105	22-23	-0.025467721886080597
1105	24-25	-0.05337720814420521
1105	26-27	-0.04591566680370107
1105	28-29	0.06301688724426668
1105	30-31	-0.04379225190221803
1105	32-33	-0.09885262055382071
1105	34-35	0.032285798748837635
1105	36-37	-0.0346245109128418
1105	38-39	-0.09459300974632612
1105	40-41	-0.12693164878615448
1105	42-43	5.058352960674029E-4
1105	44-45	0.0447963551448467
1105	46-47	0.02357886292106315
1105	48-49	0.020481718413009276
1105	50-51	0.04951809787072392
1105	52-53	-0.05982214849741041
1105	54-55	0.05425095440921979
1105	56-57	0.03440553866175833
1105	58-59	-0.001323417673731342
1105	60-61	-0.025904594246036083
1105	62-63	-0.03986090376206164
1105	64-65	0.012219168717145124
1105	66-67	-0.025063316483347364
1105	68-69	0.0717163647886494
1105	70-71	0.16393425764946556
1105	72-73	0.12255713167476046
1105	74-75	-0.011188608602338945
1105	76-77	0.014044739103198367
1105	78-79	0.07334277074959772
1105	80-81	0.043145350427131746
1105	82-83	0.09026213275027573
1105	84-85	0.12239612752043172
1105	86-87	0.10168074665696736
1105	88-89	0.17703678190653704
1105	90-91	0.1806172841065603
1105	92-93	0.11002618128099328
1105	94-95	0.15620462674153401
1105	96-97	0.05501418445461326
1105	98-99	0.12536951753295966
1105	100	0.1763044664758695
1106	1	0.0069465867925799785
1106	2	-0.017409844422999754
1106	3	-0.013295088441552139
1106	4	0.006106900677231408
1106	5	0.005618543725908864
1106	6	-0.012750408214785125
1106	7	-0.023874574492730005
1106	8	-0.01951562861130185
1106	9	0.024457416239535235
1106	10-11	-0.017119004543303618
1106	12-13	0.024223205594843478
1106	14-15	-0.04183764991520178
1106	16-17	-0.03614165658822799
1106	18-19	-0.01647709919318885
1106	20-21	-0.005955218638433735
1106	22-23	-0.019790662875749376
1106	24-25	-0.05227022740334775
1106	26-27	-0.08840786787593657
1106	28-29	-0.05657635430767982
1106	30-31	-0.05149012050663515
1106	32-33	-0.04523700028221356
1106	34-35	-0.0333357804639931
1106	36-37	-0.04084796186832307
1106	38-39	0.006186509428758313
1106	40-41	-0.11780391449141803
1106	42-43	-0.13790365044170727
1106	44-45	-0.06955077653093866
1106	46-47	-0.05327644128381337
1106	48-49	-0.09329819304348064
1106	50-51	-0.1131265325441575
1106	52-53	-0.05403693190203995
1106	54-55	-0.08594201067774065
1106	56-57	-0.019981101354680675
1106	58-59	-0.17878535703822962
1106	60-61	-0.168687830754358
1106	62-63	-0.11689394879970649
1106	64-65	-0.09122450154232098
1106	66-67	-0.04698820393594616
1106	68-69	-0.074799055606249
1106	70-71	-0.11691092195938069
1106	72-73	-0.11653361608829726
1106	74-75	-0.06420646674590813
1106	76-77	-0.06714592833557731
1106	78-79	-0.0422448107421971
1106	80-81	-0.024418675500804454
1106	82-83	-0.049421333522857935
1106	84-85	-0.011881234106972727
1106	86-87	-0.08653418618819586
1106	88-89	-0.08627313907678058
1106	90-91	-0.1146249963559498
1106	92-93	0.03427178679534393
1106	94-95	0.01842316483952544
1106	96-97	0.0033020629594631146
1106	98-99	0.03806299632174159
1106	100	0.045720901198375685
1107	1	-0.02976992094858133
1107	2	0.005399611157955064
1107	3	-0.02343077773507929
1107	4	-0.016913207928098473
1107	5	0.05468668481402972
1107	6	0.05060225369384597
1107	7	0.006239824921571824
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2316	28-29	-0.4815326927599486
2316	30-31	-0.3079377378849628
2316	32-33	-0.2394581708521315
2316	34-35	-0.29954007965457663
2316	36-37	-0.257325915370906
2316	38-39	-0.1902966124505454
2316	40-41	-0.20835517804717085
2316	42-43	-0.22085991698612162
2316	44-45	-0.22711040386851522
2316	46-47	-0.2766820869488953
2316	48-49	-0.10567793839980055
2316	50-51	-0.13176226085832354
2316	52-53	-0.24130879301201702
2316	54-55	-0.17523672949557323
2316	56-57	-0.3610089341869056
2316	58-59	-0.27713943129207763
2316	60-61	-0.1490054836821315
2316	62-63	-0.16756768041264536
2316	64-65	-0.11785242785474992
2316	66-67	-0.22487218061982617
2316	68-69	-0.17781990438853512
2316	70-71	-0.15460326422276438
2316	72-73	-0.10609358498815169
2316	74-75	-0.14444183012773948
2316	76-77	-0.22389034536515595
2316	78-79	-0.16417917685202355
2316	80-81	-0.13064625485785797
2316	82-83	-0.14724544692154495
2316	84-85	-0.083106723642139
2316	86-87	-0.1384939447575917
2316	88-89	-0.24438128848331786
2316	90-91	-0.3158430703940738
2316	92-93	-0.2650606387013781
2316	94-95	-0.14589786775719205
2316	96-97	-0.10805074841276507
2316	98-99	-0.0693342091594289
2316	100	-0.18848916859717946
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	12.0
14	66.0
15	273.0
16	588.0
17	1516.0
18	2965.0
19	4870.0
20	7353.0
21	11973.0
22	16087.0
23	16706.0
24	15914.0
25	16632.0
26	20087.0
27	25235.0
28	32072.0
29	40235.0
30	49983.0
31	63742.0
32	82086.0
33	110694.0
34	173670.0
35	265181.0
36	453117.0
37	831445.0
38	797717.0
39	176826.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02939841611884	21.862484099638536	14.259810973767356	22.84830651047528
2	15.213968932706601	21.48883832998917	39.832826034164256	23.46436670313997
3	17.903951577908167	28.369552918766423	30.347206939040934	23.37928856428448
4	12.72171875514839	18.479334640130357	39.91621454100775	28.882732063713508
5	13.15152521233848	39.34658004636909	34.24725456805183	13.254640173240595
6	28.54637469471416	38.6437086949075	18.96907959952342	13.840837010854917
7	26.114817775909938	33.22771825423963	23.456214866640888	17.201249103209545
8	26.81425659521251	34.14576598594709	20.380646727643672	18.659330691196736
9	26.81945007423028	15.503093550047623	21.582049949394097	36.095406426328005
10-11	25.559652285491946	26.122820975457593	27.676022659788142	20.641504079262322
12-13	26.17350716460278	23.898685945516814	27.775603609002737	22.152203280877668
14-15	23.85083589418079	25.025567028511354	25.4882136775676	25.63538339974026
16-17	22.538773695899835	27.66902440578602	26.467587692508545	23.324614205805602
18-19	22.10604534400446	27.52731984583915	28.585361122737606	21.781273687418782
20-21	23.677684473269892	26.605586147622134	27.937141462792948	21.779587916315027
22-23	24.051287263946694	25.991951550147235	27.591476824648424	22.36528436125765
24-25	23.425864404329776	26.462782801825597	27.878375301423443	22.23297749242119
26-27	22.965269580205735	26.942011832480183	27.350561121917444	22.74215746539664
28-29	22.34144767537824	26.961083423659048	28.047230064956803	22.650238836005908
30-31	23.334318722835732	26.381099545477436	27.841776287363203	22.44280544432363
32-33	23.59224957818	26.822467556850892	27.095826810717522	22.489456054251587
34-35	23.376756191860483	27.019896513758273	27.649122828831167	21.954224465550073
36-37	23.300613239475805	26.688390259975925	27.268274979347563	22.74272152120071
38-39	23.42524369142403	26.555988995438184	27.706522430086512	22.312244883051275
40-41	23.142611346021884	25.833675920536347	28.295982909237576	22.727729824204193
42-43	22.974295145992624	26.040310411023075	27.67882913078976	23.30656531219454
44-45	23.065952257831274	26.560624422293895	27.568279091324932	22.8051442285499
46-47	23.0468890246611	26.368830237089675	26.972655645907455	23.611625092341768
48-49	23.675924961646132	26.086257734891216	27.396895318418718	22.840921985043934
50-51	23.478099895735994	26.276223765354384	26.90740336616686	23.338272972742764
52-53	22.924221476682185	26.486757856730414	27.023375710989516	23.565644955597882
54-55	22.999523300976758	26.303698777197837	27.020938853053135	23.675839068772266
56-57	23.64558273910866	26.057209078076443	26.595562797779376	23.701645385035516
58-59	22.78844285698743	26.50100951697061	27.44992438337087	23.260623242671095
60-61	23.21842775142568	26.800480788286375	26.77205191929061	23.20903954099734
62-63	23.421896883559626	26.23236473118761	26.688230533496654	23.657507851756108
64-65	22.50788710704737	26.12644425993617	27.224422194998443	24.141246438018015
66-67	23.242021751145657	26.116249198771254	26.855369118545795	23.786359931537294
68-69	22.617621048148823	26.155647877451234	27.19462667916276	24.032104395237187
70-71	22.65868352447998	25.875725902101856	27.19865785727486	24.266932716143312
72-73	22.90453454462595	26.435998195744332	26.939240664515694	23.720226595114024
74-75	23.00730429841167	26.608409059363105	26.894857924999794	23.489428717225433
76-77	22.623120567199514	26.506064170537858	26.572431835589732	24.298383426672903
78-79	22.482366583881845	26.366419752472392	27.00778324478481	24.14343041886095
80-81	22.443526402009333	26.92947444661003	26.93937500582843	23.687624145552206
82-83	23.05750052418176	26.2232008675383	27.602642093668212	23.11665651461173
84-85	23.036764928733902	26.403993792338653	26.706030554936017	23.85321072399143
86-87	21.957276976916674	27.058624400597587	27.213165000183402	23.77093362230234
88-89	22.52123686062502	25.928413253392552	27.325967781643296	24.224382104339135
90-91	22.656208108480705	25.947955783226963	27.309373333567503	24.086462774724826
92-93	22.668033132124716	26.211782252050707	27.200791157389403	23.919393458435174
94-95	21.829659223638227	26.0491281841658	27.602053781598805	24.519158810597162
96-97	22.0160239577581	26.072759504948017	27.39478497871694	24.51643155857694
98-99	21.520299779610387	26.500747583951657	27.304952083132576	24.67400055330538
100	21.762151674180238	26.118324548416304	26.952842449349053	25.166681328054402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	36.0
2	39.5
3	63.0
4	51.5
5	54.5
6	95.5
7	182.5
8	340.0
9	447.0
10	437.0
11	462.5
12	546.0
13	644.5
14	774.0
15	936.0
16	1146.0
17	1432.5
18	1761.0
19	2036.5
20	2291.5
21	2609.5
22	3050.5
23	3454.5
24	3810.5
25	4318.5
26	5124.5
27	6254.5
28	7735.0
29	10516.0
30	14990.5
31	20268.0
32	28346.0
33	39332.5
34	50014.0
35	60213.0
36	75076.0
37	95602.0
38	106380.5
39	108128.0
40	106863.5
41	103039.0
42	102817.5
43	107725.0
44	116262.0
45	132687.0
46	146506.5
47	154617.5
48	160325.5
49	156462.0
50	149399.5
51	145224.0
52	140738.5
53	134561.5
54	129067.0
55	115828.5
56	100981.5
57	82735.5
58	64465.5
59	56365.0
60	44836.5
61	31562.0
62	23477.0
63	18040.5
64	12367.5
65	6689.5
66	4143.0
67	3023.5
68	2159.0
69	1569.5
70	1120.0
71	769.0
72	537.5
73	391.5
74	285.5
75	165.5
76	93.0
77	56.5
78	30.5
79	15.5
80	11.0
81	7.5
82	5.5
83	3.0
84	2.0
85	2.0
86	1.5
87	0.5
88	1.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004973472739774229
2	0.031612636102189944
3	7.460209109661344E-4
4	0.0013055365941907352
5	0.008392735248369011
6	3.108420462358893E-5
7	0.0012433681849435573
8	0.007056114449554688
9	0.0016163786404266244
10-11	1.0879471618256126E-4
12-13	6.216840924717786E-5
14-15	0.0010568629572020236
16-17	9.32526138707668E-5
18-19	0.0011967418780081738
20-21	9.32526138707668E-5
22-23	0.0015852944358030357
24-25	0.0012589102872553517
26-27	0.002346857449080964
28-29	2.175894323651225E-4
30-31	0.0020515575051568698
32-33	0.00174071545892098
34-35	6.838525017189565E-4
36-37	0.0042119097264963
38-39	0.0011967418780081738
40-41	0.0039010676802604108
42-43	0.00334155199703581
44-45	0.004429499158861423
46-47	0.002440110062951731
48-49	0.009729356047183337
50-51	0.007273703881919811
52-53	0.004989014842086023
54-55	0.004771425409720901
56-57	0.004072030805690151
58-59	0.005688409446116775
60-61	0.008315024736810039
62-63	0.003496973020153755
64-65	0.003388178303971194
66-67	0.004165283419560917
68-69	0.0019583048912861028
70-71	0.003807815066389644
72-73	0.007118282858801866
74-75	0.008190687918315684
76-77	0.004351788647302451
78-79	0.0029063731323055652
80-81	0.002424567960639937
82-83	0.004647088591226546
84-85	0.002346857449080964
86-87	0.003543599327089138
88-89	6.216840924717787E-4
90-91	0.0016630049473620077
92-93	2.7975784161230036E-4
94-95	0.002346857449080964
96-97	0.0022536048352101976
98-99	0.0011811997756963793
100	3.108420462358893E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3217068.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.57938571986432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03563664055966	49.103347070661506
2	14.989398790936248	19.360123324574793
3	4.552921494427173	8.820746200226205
4	1.8812527355019097	4.859605841701108
5	0.8984186220091722	2.900966136431966
6	0.4679770984470366	1.8133004129204453
7	0.2967280777348209	1.3413761890165616
8	0.18971542565668809	0.9801364520393202
9	0.13065489891392118	0.7593851801836798
>10	0.5056196125297943	5.384007758291295
>50	0.03047016081105276	1.3715840418559782
>100	0.019911287030277187	2.336466202669388
>500	0.001013599911392633	0.424892941803649
>1k	2.2524442470742338E-4	0.35092578634936483
>5k	5.6311106176855844E-5	0.19313646127476441
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5311	0.16508821075588082	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4597	0.14289408865463832	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.108420462358893E-5	0.0
2	0.0	0.0	0.0	3.108420462358893E-5	0.0
3	0.0	0.0	0.0	3.108420462358893E-5	0.0
4	0.0	0.0	0.0	3.108420462358893E-5	0.0
5	0.0	0.0	0.0	3.108420462358893E-5	0.0
6	0.0	0.0	0.0	3.108420462358893E-5	3.108420462358893E-5
7	0.0	0.0	0.0	3.108420462358893E-5	3.108420462358893E-5
8	0.0	0.0	0.0	3.108420462358893E-5	3.108420462358893E-5
9	0.0	0.0	0.0	1.5542102311794467E-4	3.108420462358893E-5
10-11	0.0	0.0	0.0	1.865052277415336E-4	3.108420462358893E-5
12-13	0.0	0.0	0.0	2.1758943236512254E-4	3.108420462358893E-5
14-15	0.0	0.0	0.0	2.3313153467691697E-4	4.66263069353834E-5
16-17	0.0	0.0	0.0	3.263841485476838E-4	6.216840924717786E-5
18-19	0.0	0.0	0.0	4.040946601066561E-4	1.0879471618256126E-4
20-21	0.0	0.0	0.0	4.818051716656284E-4	1.2433681849435572E-4
22-23	0.0	0.0	0.0	5.905998878481897E-4	1.2433681849435572E-4
24-25	0.0	0.0	0.0	8.548156271486957E-4	1.5542102311794467E-4
26-27	0.0	0.0	0.0	0.0013521629011261187	1.865052277415336E-4
28-29	0.0	0.0	0.0	0.002067099607468664	2.0204733005332805E-4
30-31	0.0	0.0	0.0	0.005657325241493186	2.1758943236512254E-4
32-33	0.0	0.0	0.0	0.01502921293550525	2.1758943236512254E-4
34-35	0.0	0.0	0.0	0.03044697842880536	2.4867363698871143E-4
36-37	0.0	0.0	0.0	0.061391304131588144	2.4867363698871143E-4
38-39	0.0	0.0	0.0	0.11271132596513347	2.4867363698871143E-4
40-41	0.0	0.0	0.0	0.17604539288569593	2.4867363698871143E-4
42-43	0.0	0.0	0.0	0.24098029634437318	2.4867363698871143E-4
44-45	0.0	0.0	0.0	0.3206180285900081	2.4867363698871143E-4
46-47	0.0	0.0	0.0	0.4201651938970516	2.4867363698871143E-4
48-49	0.0	0.0	0.0	0.5346638616280415	2.797578416123004E-4
50-51	0.0	0.0	0.0	0.6634146371789468	2.797578416123004E-4
52-53	0.0	0.0	0.0	0.7964394908655956	3.730104554830672E-4
54-55	0.0	1.5542102311794465E-5	0.0	0.943343441916677	4.66263069353834E-4
56-57	0.0	3.108420462358893E-5	0.0	1.1169020984324858	4.818051716656284E-4
58-59	0.0	3.108420462358893E-5	0.0	1.3271556585064412	4.973472739774229E-4
60-61	0.0	3.108420462358893E-5	0.0	1.5549562520904128	4.973472739774229E-4
62-63	0.0	3.108420462358893E-5	0.0	1.7922375280845788	4.973472739774229E-4
64-65	0.0	3.108420462358893E-5	0.0	2.032223129880997	4.973472739774229E-4
66-67	0.0	3.108420462358893E-5	0.0	2.29892560555139	4.973472739774229E-4
68-69	0.0	3.108420462358893E-5	0.0	2.6052449000145472	4.973472739774229E-4
70-71	3.108420462358893E-5	3.108420462358893E-5	0.0	2.9548178652114285	5.595156832246008E-4
72-73	3.108420462358893E-5	3.108420462358893E-5	0.0	3.3128768182705493	6.216840924717787E-4
74-75	3.108420462358893E-5	3.108420462358893E-5	0.0	3.668775419108331	6.216840924717787E-4
76-77	3.108420462358893E-5	3.108420462358893E-5	0.0	4.068751422102361	6.216840924717787E-4
78-79	3.108420462358893E-5	3.108420462358893E-5	0.0	4.503666071093306	6.216840924717787E-4
80-81	3.108420462358893E-5	3.108420462358893E-5	0.0	4.971840819031491	6.372261947835731E-4
82-83	3.108420462358893E-5	3.108420462358893E-5	0.0	5.462256315377854	6.838525017189565E-4
84-85	3.108420462358893E-5	3.108420462358893E-5	0.0	5.94586437091165	6.838525017189565E-4
86-87	3.108420462358893E-5	3.108420462358893E-5	0.0	6.45227269053685	6.838525017189565E-4
88	3.108420462358893E-5	3.108420462358893E-5	0.0	6.848565215282984	6.838525017189565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5850	0.0	51.034145	1
GTATCAA	10555	0.0	39.331947	1
TACCCCG	220	0.0	34.180485	5
CAACGCA	12185	0.0	32.900642	5
ATCAACG	12635	0.0	31.767557	3
TATCAAC	13475	0.0	30.600412	2
AACGCAG	14235	0.0	28.952715	6
GTACTAG	2565	0.0	28.777645	1
TCAACGC	14615	0.0	27.560251	4
GTGGTAT	1895	0.0	27.539545	1
TATTCTC	1555	0.0	27.50375	5
ACGCAGA	14820	0.0	26.921957	7
GCTATTG	3175	0.0	26.643389	8
CGCAGAG	14975	0.0	26.518597	8
GGGCTAT	3485	0.0	26.025656	6
AGGGCTA	3305	0.0	25.880987	5
CTATTGA	3305	0.0	25.452002	9
GGCTATT	3545	0.0	25.320032	7
ATTTAGG	3475	0.0	25.029943	1
GCAGAGT	16460	0.0	23.953693	9
>>END_MODULE