FastQCFastQC Report
Thu 26 May 2016
ERR580220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529122
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144850.22185218778267585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120270.18420547203743476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66100.10123872704476958No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89750.047.884931
GTATCAA156450.040.0027281
CAACGCA185850.032.6085
ATCAACG188150.032.2865833
TATCAAC198550.031.1654282
TCAACGC197450.030.9320834
AACGCAG200000.030.6390366
ACGCAGA222650.027.0780967
CGCAGAG229900.026.0604578
GTACATG208850.025.3507611
GTGGTAT42300.025.2332171
TACATGG213900.024.3126032
GTACTAG26250.023.107211
ACATGGG217850.022.9424343
GCAGAGT259200.022.520989
TGGTATC46600.022.2994162
CATGGGG161100.020.6922554
GAGTACT138800.020.49272512-13
CTAGTAC34900.020.4777373
CTAACGC13900.019.9572643
Produced by FastQC (version 0.11.5)