FastQCFastQC Report
Thu 26 May 2016
ERR580220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529122
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128090.1961825801386465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112190.1718301480658502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92600.050.2215731
GTATCAA159200.038.721921
ATCAACG188250.031.7066523
CAACGCA187800.031.6565255
TATCAAC199900.030.5403942
TCAACGC199100.030.0491454
AACGCAG204050.029.5026426
ACGCAGA227550.026.0633877
CGCAGAG235850.024.8871368
GTACATG207400.024.5378821
GTGGTAT42250.024.0013751
TACATGG209800.023.6109492
ACATGGG212900.022.4063953
GCAGAGT262750.021.8741979
TAGGCAT41000.021.2058755
CATGGGG162300.020.5885624
GAGTACT142050.020.3954612-13
TAGTACT42400.020.1734664
GCATCGT32750.019.9458188
CTAACGC11600.019.8527053
Produced by FastQC (version 0.11.5)