FastQCFastQC Report
Thu 26 May 2016
ERR580219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6919124
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203770.29450259888390495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163110.23573793445528657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98940.14299498028941235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115800.051.906681
GTATCAA212650.042.332851
CAACGCA248900.035.301545
ATCAACG249550.035.0016023
TATCAAC268150.033.3336372
AACGCAG274800.032.419146
TCAACGC280150.031.3975334
GTACTAG43750.030.5310151
ACGCAGA294600.029.5229577
GTGGTAT38500.028.952671
CGCAGAG301150.028.8021958
GTACATG298000.028.4564321
TACATGG304500.027.4242062
ACATGGG300600.027.0712263
GCAGAGT329050.025.7200959
GCATCGT46900.025.4470628
CATGGGG238700.024.9734884
TAGTACT60150.024.8544334
CTGTGCG60900.024.846169
TAGGCAT58950.023.8449615
Produced by FastQC (version 0.11.5)