FastQCFastQC Report
Thu 26 May 2016
ERR580219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6919124
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179890.2599895593719667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151310.2186837524519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92930.13430890962497563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116100.052.967511
GTATCAA208650.040.7618751
CAACGCA249450.032.934475
ATCAACG253300.032.4897883
TATCAAC266950.031.75412
AACGCAG275100.030.4936226
TCAACGC278550.029.6796344
GTACTAG42800.028.4505081
GTGGTAT39850.027.371191
ACGCAGA302400.027.1190837
GTACATG302550.026.7900851
CGCAGAG309500.026.4362288
TACATGG306000.026.2422122
TAGTACT60000.025.3798854
ACATGGG307450.025.3610823
TAGGCAT56050.024.7365535
CTGTGCG60000.024.5157419
GGGCACC54650.024.07918594
CATGGGG241950.023.6601544
GCAGAGT343300.023.4769829
Produced by FastQC (version 0.11.5)