FastQCFastQC Report
Thu 26 May 2016
ERR580218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7080564
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181980.2570134243543311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145700.2057745682406091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82370.1163325407411048No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103150.049.69961
GTATCAA188150.042.8702051
CAACGCA220750.035.180775
ATCAACG223550.034.8232573
TATCAAC232050.034.4241372
AACGCAG241000.032.8322036
TCAACGC239950.032.542744
ACGCAGA263050.029.4376477
CGCAGAG272100.028.3557458
GTGGTAT42650.027.2378981
GTACATG253050.026.076361
TACATGG255550.025.3527242
GTACTAG33100.025.150171
GCAGAGT304800.024.7145029
ACATGGG256950.024.5703373
TCTAACG17600.022.7068842
CATGGGG202700.022.450544
CTGTGCG44500.022.0708759
CTAGTAC48750.021.7930263
TAACGCC17700.021.7793944
Produced by FastQC (version 0.11.5)