FastQCFastQC Report
Thu 26 May 2016
ERR580218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7080564
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165310.23347010209921132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132580.18724497088085074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79570.11237805349969296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102850.050.6497731
GTATCAA184150.041.513641
CAACGCA219400.033.4836245
ATCAACG221500.033.208613
TATCAAC230600.032.8266832
TCAACGC238000.031.0643254
AACGCAG241850.031.0342586
ACGCAGA267500.027.320537
GTACTAG34050.027.2013931
GTGGTAT42150.026.6589091
CGCAGAG277500.026.3023198
GTACATG240200.024.9953921
TACATGG246900.024.1848342
TAGTACT47950.023.623194
ACATGGG246900.023.243673
CTGTGCG46100.023.1407179
GCAGAGT312250.022.876689
CTAGTAC49200.021.7811033
CATGGGG192350.021.3075964
GAGTACT174150.021.15690412-13
Produced by FastQC (version 0.11.5)