FastQCFastQC Report
Thu 26 May 2016
ERR580217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6799433
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144930.21315012590020374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119690.17602938362654652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73010.10737660037241342No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93850.043.442751
GTATCAA160050.038.137441
CAACGCA175700.032.946255
GTACTAG50250.032.473261
ATCAACG183750.031.6327133
TAGTACT70350.030.2143464
TATCAAC202350.029.9339732
CTGTGCG72700.029.4818789
GTACATG253650.029.4406991
AACGCAG202050.029.4046556
TACATGG257400.028.5928522
TCAACGC210350.027.8333644
GCATCGT52400.027.7161398
CTAGTAC79300.027.694213
ACATGGG253000.027.6398533
GTGGTAT33500.027.0922471
GGGCACC67200.026.9275694
TAGGCAT63550.026.6383085
TCTAACG24300.026.3199042
ACGCAGA221250.026.1727267
Produced by FastQC (version 0.11.5)