FastQCFastQC Report
Thu 26 May 2016
ERR580217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6799433
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131750.19376615667806418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112840.1659550141901538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72740.10697950843842421No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99500.044.0328831
GTATCAA163700.036.7000271
CAACGCA183850.031.2656365
ATCAACG192750.029.8949133
TAGTACT71500.029.3834464
CTGTGCG73550.029.13839
GGGCACC68550.028.4576794
TATCAAC211500.028.4232772
GTACATG247000.028.2056121
AACGCAG211700.027.994736
CTAACGC22400.027.9064663
TACATGG251300.027.4005432
CTAGTAC77850.027.3489593
ACATGGG248350.026.7221723
TCAACGC218500.026.6084444
TAACGCC22900.026.2709454
TAGGCAT64850.026.0188965
GTACTGT86250.025.9920966
TCTAACG23000.025.9531362
CATGGGG211400.025.1898824
Produced by FastQC (version 0.11.5)