FastQCFastQC Report
Thu 26 May 2016
ERR580216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7168228
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121470.1694561054698595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96900.13517985198015464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104250.045.51471
GTATCAA193250.042.4388471
CAACGCA218150.035.9439435
ATCAACG227200.034.6331983
TATCAAC240700.033.692582
AACGCAG247600.032.4278766
GTACTAG48250.031.6754421
TCAACGC257350.030.8342344
ACGCAGA266300.029.216317
CGCAGAG275550.028.1161888
GTGGTAT40150.027.641591
GTACATG337200.026.8878481
TAGGCAT64900.026.5831385
TACATGG348550.025.7760832
GCATCGT54650.025.6190078
TAGTACT63950.025.360834
ACATGGG344300.025.2432543
GCAGAGT299500.025.2288939
CTAGGCA69600.024.990644
CTGTGCG66950.024.4251739
Produced by FastQC (version 0.11.5)