FastQCFastQC Report
Thu 26 May 2016
ERR580216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7168228
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105500.14717723822400738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88400.12332197022750951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104400.045.9815331
GTATCAA190750.041.7795071
CAACGCA219100.034.860435
ATCAACG226200.033.7671743
TATCAAC241650.032.8048482
GTACTAG49150.031.663771
AACGCAG249300.031.426926
TCAACGC257200.029.9348344
ACGCAGA270150.028.0967467
GTGGTAT39000.027.6076241
CGCAGAG280550.026.921188
GTACATG332750.026.9175221
TAGGCAT63800.026.7428535
TACATGG343300.025.9402072
ACATGGG342100.025.102643
CTAGGCA70200.024.506314
GCAGAGT304800.024.2687829
CTGTGCG65400.024.1445859
TAGTACT64800.023.9371744
CATGGGG270700.023.2501754
Produced by FastQC (version 0.11.5)