FastQCFastQC Report
Thu 26 May 2016
ERR580215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6563088
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220900.3365793662983035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180570.27512963409907043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104350.15899527783263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113600.055.0987741
GTATCAA212000.042.2350731
CAACGCA246600.035.1131485
ATCAACG249300.034.7310033
TATCAAC262900.033.6194152
AACGCAG271450.032.4202086
TCAACGC276850.031.5144654
ACGCAGA293250.029.4186277
CGCAGAG297050.028.8362128
GTGGTAT38700.028.799141
GTACTAG41350.028.0907671
GCAGAGT322250.025.8689149
GTACATG268900.025.410721
TACATGG270700.024.8701062
GCATCGT41050.024.8386338
ACATGGG269050.024.337063
TAGGCAT53650.023.8326535
CTGTGCG51850.022.929229
CATGGGG208600.022.6479194
GAGTACT188700.022.56240812-13
Produced by FastQC (version 0.11.5)