FastQCFastQC Report
Thu 26 May 2016
ERR580215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6563088
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194550.296430582676935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167430.255108570843481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100930.15378431616336702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115950.053.809771
GTATCAA212850.041.219141
CAACGCA254900.033.15475
ATCAACG255600.033.0830463
TATCAAC270200.032.211322
AACGCAG279500.030.637886
TCAACGC282700.030.0943264
GTACTAG38950.029.3332211
GTGGTAT40800.028.1184021
ACGCAGA304200.027.7639337
CGCAGAG308850.027.2844338
CTGTGCG53000.024.9156939
GCAGAGT336200.024.7550289
GTACATG269150.024.3167651
TACATGG267200.024.1261732
TAGTACT57100.024.036914
ACATGGG266250.023.4799523
GGGCACC52600.022.87370394
TGGTATC49400.022.8439792
GAGTACT191900.022.27324312-13
Produced by FastQC (version 0.11.5)