##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580215_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6563088
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.476134709758576	34.0	31.0	34.0	31.0	34.0
2	32.61006465249285	34.0	31.0	34.0	31.0	34.0
3	32.65600156511691	34.0	31.0	34.0	31.0	34.0
4	36.19099393456251	37.0	35.0	37.0	35.0	37.0
5	36.13602057446129	37.0	35.0	37.0	35.0	37.0
6	36.171555523863155	37.0	36.0	37.0	35.0	37.0
7	36.118933495939714	37.0	36.0	37.0	35.0	37.0
8	36.114117470312756	37.0	36.0	37.0	35.0	37.0
9	37.8217822159325	39.0	38.0	39.0	35.0	39.0
10-11	37.77022477833605	39.0	38.0	39.0	35.0	39.0
12-13	37.731241833112705	39.0	38.0	39.0	35.0	39.0
14-15	39.088350026694755	40.0	38.0	41.0	36.0	41.0
16-17	39.08319239358058	40.0	38.0	41.0	36.0	41.0
18-19	39.083264691864564	40.0	38.5	41.0	35.5	41.0
20-21	39.057626684268136	40.0	39.0	41.0	35.0	41.0
22-23	38.977667753350254	40.0	38.0	41.0	35.0	41.0
24-25	38.903455964631284	40.0	38.0	41.0	35.0	41.0
26-27	38.771574447881854	40.0	38.0	41.0	35.0	41.0
28-29	38.66835382978256	40.0	38.0	41.0	34.5	41.0
30-31	38.507885236339966	40.0	38.0	41.0	34.0	41.0
32-33	38.38801704319674	40.0	38.0	41.0	34.0	41.0
34-35	38.287835467085	40.0	38.0	41.0	34.0	41.0
36-37	38.16044916661181	40.0	38.0	41.0	34.0	41.0
38-39	38.038766888391564	40.0	37.0	41.0	33.0	41.0
40-41	37.923766144839135	40.0	37.0	41.0	33.0	41.0
42-43	37.91999208299508	40.0	37.0	41.0	33.0	41.0
44-45	37.86919137759543	40.0	37.0	41.0	33.0	41.0
46-47	37.67205231744569	40.0	37.0	41.0	33.0	41.0
48-49	37.461421970877126	40.0	37.0	41.0	32.5	41.0
50-51	37.26822381476525	40.0	36.0	41.0	32.0	41.0
52-53	37.1001920132718	39.5	36.0	41.0	31.5	41.0
54-55	36.88920566050615	39.0	35.0	41.0	31.0	41.0
56-57	36.652198020200245	39.0	35.0	41.0	31.0	41.0
58-59	36.41042516876202	38.5	35.0	40.5	31.0	41.0
60-61	36.162474813685265	38.0	35.0	40.0	30.0	41.0
62-63	35.86495099562889	37.5	35.0	40.0	30.0	41.0
64-65	35.57318727099195	37.0	35.0	40.0	29.0	41.0
66-67	35.26112692683688	36.5	34.0	39.5	29.0	41.0
68-69	34.95141791485959	36.0	34.0	39.0	29.0	41.0
70-71	34.639808654096974	35.5	34.0	39.0	29.0	40.5
72-73	34.32917362985229	35.0	34.0	38.5	28.0	40.0
74-75	33.98106714400295	35.0	34.0	37.5	28.0	39.5
76-77	32.45275821381642	34.0	31.5	36.0	25.5	39.0
78-79	33.355449218416695	35.0	33.0	36.0	27.0	39.0
80-81	33.33709010148881	35.0	33.0	36.0	28.0	38.5
82-83	33.10421572893735	35.0	33.0	36.0	27.0	37.0
84-85	32.88105652400212	35.0	33.0	35.0	27.0	37.0
86-87	32.67127722498921	35.0	33.0	35.0	27.0	36.5
88-89	32.48542568681084	35.0	33.0	35.0	26.0	36.0
90-91	32.31175873308418	35.0	33.0	35.0	26.0	36.0
92-93	32.14668201919584	35.0	32.5	35.0	25.5	36.0
94-95	32.007237599130164	35.0	32.0	35.0	25.0	35.0
96-97	31.87824649006687	34.5	32.0	35.0	25.0	35.0
98-99	31.77137187555614	34.0	32.0	35.0	25.0	35.0
100	29.88573488577328	33.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.04254808514979658
1101	2	-0.06436249765815205
1101	3	-0.059415428973984774
1101	4	-0.04186423004952644
1101	5	-0.08970486031589786
1101	6	-0.10260678617444796
1101	7	-0.09605316624283233
1101	8	-0.08320287893921119
1101	9	-0.11704524472528988
1101	10-11	-0.06373505308908278
1101	12-13	-0.04873510977997597
1101	14-15	-0.0816554350164509
1101	16-17	-0.032739360680416496
1101	18-19	-0.039068230877710164
1101	20-21	-0.07411612720384397
1101	22-23	-0.07622268892939843
1101	24-25	-0.11017290781649791
1101	26-27	-0.13445476642249332
1101	28-29	-0.15207946642767212
1101	30-31	-0.19143457552719667
1101	32-33	-0.16160138080078923
1101	34-35	-0.2214813695432909
1101	36-37	-0.16259927580160394
1101	38-39	-0.17038565218845747
1101	40-41	-0.14037719906490764
1101	42-43	-0.19379479237659325
1101	44-45	-0.1463095433282504
1101	46-47	-0.17960576647176651
1101	48-49	-0.145496409849585
1101	50-51	-0.08511395175083436
1101	52-53	-0.06213966969800566
1101	54-55	-0.07463793158857612
1101	56-57	-0.17599236927813422
1101	58-59	-0.24360368264821375
1101	60-61	-0.15846202064712855
1101	62-63	-0.14671771440422532
1101	64-65	-0.23531008517968388
1101	66-67	-0.182810833617431
1101	68-69	-0.2272627079244387
1101	70-71	-0.31122900345052784
1101	72-73	-0.20121336536815448
1101	74-75	-0.21720245351541934
1101	76-77	-0.2165086141548329
1101	78-79	-0.19113994030350057
1101	80-81	-0.17073385748029324
1101	82-83	-0.19062652698345062
1101	84-85	-0.17091063898136838
1101	86-87	-0.19334351345253253
1101	88-89	-0.24032109811024327
1101	90-91	-0.17466413504686784
1101	92-93	-0.21844757780954183
1101	94-95	-0.27236862057497646
1101	96-97	-0.2947883355639682
1101	98-99	-0.3334661183960499
1101	100	-0.48268149306794683
1102	1	-0.0722394018218111
1102	2	-0.13159181113551455
1102	3	-0.07615692656362683
1102	4	-0.04931262979797424
1102	5	-0.007486877914196555
1102	6	-0.053324737286168045
1102	7	-0.04075856853449267
1102	8	-0.021554466469588363
1102	9	-0.03504331705745756
1102	10-11	-0.03552618889477088
1102	12-13	-0.0674958368564802
1102	14-15	-0.060514181796406774
1102	16-17	-0.04561240390264487
1102	18-19	-0.04633891893919895
1102	20-21	-0.06837600637322794
1102	22-23	-0.14663768124027143
1102	24-25	-0.20634215530791522
1102	26-27	-0.1364956039474876
1102	28-29	-0.15507970084603784
1102	30-31	-0.22991120755602878
1102	32-33	-0.15178822813544457
1102	34-35	-0.16806613255504743
1102	36-37	-0.24020038346683492
1102	38-39	-0.31020531675989105
1102	40-41	-0.1917236683755945
1102	42-43	-0.17617262296546699
1102	44-45	-0.15141915827685892
1102	46-47	-0.10288956246931491
1102	48-49	-0.12041772324897693
1102	50-51	-0.11121514097518315
1102	52-53	-0.1397091283198293
1102	54-55	-0.16571981335759745
1102	56-57	-0.13608078398421952
1102	58-59	-0.17086558899782034
1102	60-61	-0.20260970472710227
1102	62-63	-0.11504975595731537
1102	64-65	-0.19811113580481532
1102	66-67	-0.19436217021244317
1102	68-69	-0.2526254023711303
1102	70-71	-0.17322960770616902
1102	72-73	-0.2283524713775762
1102	74-75	-0.26239694124718227
1102	76-77	-0.22212906364306662
1102	78-79	-0.13159116230537649
1102	80-81	-0.13689587493497157
1102	82-83	-0.12736986093574387
1102	84-85	-0.1343636881127992
1102	86-87	-0.19149834088060658
1102	88-89	-0.17017353252182943
1102	90-91	-0.14312569659117713
1102	92-93	-0.1531349293744988
1102	94-95	-0.21482904388221158
1102	96-97	-0.1965744803616758
1102	98-99	-0.22903080247498764
1102	100	-0.21806389250657432
1103	1	-0.0069200589649227595
1103	2	-0.004221055217954017
1103	3	0.012645103650783085
1103	4	0.01749870274664289
1103	5	-0.03777743518835308
1103	6	-0.0155825150153035
1103	7	0.01659476479220956
1103	8	-0.03540599833422675
1103	9	-0.10400272271925104
1103	10-11	-0.019490465120945544
1103	12-13	-0.002289613976664384
1103	14-15	-0.06622534140863223
1103	16-17	-0.046774548423591966
1103	18-19	-0.020087062005437417
1103	20-21	-0.04337940183818034
1103	22-23	-0.06577814105338575
1103	24-25	-0.052705525280757115
1103	26-27	-0.06230475692698434
1103	28-29	-0.055833295063841604
1103	30-31	-0.11705281809594936
1103	32-33	-0.12409190558568639
1103	34-35	-0.04446314872492252
1103	36-37	-0.051815547687930064
1103	38-39	-0.029499754067913386
1103	40-41	-0.024931340484620534
1103	42-43	-0.008374267315758743
1103	44-45	0.010845522320586554
1103	46-47	-0.004917799305111714
1103	48-49	-0.07832139552516537
1103	50-51	-0.02440146021157119
1103	52-53	0.010924890806215615
1103	54-55	0.017030615242113356
1103	56-57	0.08473531912647303
1103	58-59	-0.009042094967377068
1103	60-61	-0.10260420730764963
1103	62-63	0.007349790518020427
1103	64-65	0.049416679645069905
1103	66-67	0.04109235443738868
1103	68-69	-0.05455094193911236
1103	70-71	-0.021144481285425343
1103	72-73	0.007089702734901948
1103	74-75	0.053893392647665905
1103	76-77	0.028236800814951835
1103	78-79	0.048571436934231826
1103	80-81	0.016599463949852122
1103	82-83	0.04013097487100481
1103	84-85	0.037658526436814554
1103	86-87	-0.013435656368798732
1103	88-89	-0.005259170376710642
1103	90-91	0.007313052206107784
1103	92-93	-0.011709137571969563
1103	94-95	-0.05473287137965599
1103	96-97	-0.12290570162459957
1103	98-99	-0.09423387864186239
1103	100	0.07871932111070734
1104	1	0.006507168234229255
1104	2	0.022755032243559015
1104	3	3.9339454655618056E-4
1104	4	0.0307358859033684
1104	5	0.001456944044662123
1104	6	9.168917644757357E-4
1104	7	0.01993127503454417
1104	8	0.020977935783704993
1104	9	0.00795525801878938
1104	10-11	0.019081551502097227
1104	12-13	-0.034956478253377554
1104	14-15	-0.035790293903225745
1104	16-17	-0.04240153626721366
1104	18-19	-0.0014485199716460784
1104	20-21	-0.015743718924973393
1104	22-23	-0.06530039788039232
1104	24-25	-0.004738287069535829
1104	26-27	-0.08062013875777296
1104	28-29	-0.09113943102644839
1104	30-31	-0.05921370004463711
1104	32-33	-0.06216946149885416
1104	34-35	0.006337976511353816
1104	36-37	-0.0249554005014474
1104	38-39	-0.04201692751544783
1104	40-41	-0.06972860993572283
1104	42-43	-0.026360942436852497
1104	44-45	-0.11227187140737271
1104	46-47	-0.07578132188834275
1104	48-49	-0.015231673867646123
1104	50-51	-0.01881509181352925
1104	52-53	-0.13613350535177204
1104	54-55	-0.08906129468905277
1104	56-57	-0.11654746962685891
1104	58-59	-0.14380729902713796
1104	60-61	-0.16821665086980886
1104	62-63	-0.07059106241231916
1104	64-65	-0.08310251833835736
1104	66-67	-0.07364870488638786
1104	68-69	-0.06970609491933288
1104	70-71	-0.04505374063101186
1104	72-73	-0.006620664488217187
1104	74-75	-0.02961965156580959
1104	76-77	0.00996336520289276
1104	78-79	0.003515980644216654
1104	80-81	0.1209610466454123
1104	82-83	0.0710253462711421
1104	84-85	0.04591197219494347
1104	86-87	0.10089900858123713
1104	88-89	0.09867837883481911
1104	90-91	0.12958078205968349
1104	92-93	0.13054549618694722
1104	94-95	0.06408156683796307
1104	96-97	3.8563299120042416E-4
1104	98-99	-0.008600398825489464
1104	100	0.23845561750900401
1105	1	-0.011693546133990651
1105	2	0.029591242917433647
1105	3	0.003006226639151066
1105	4	0.012978045284263828
1105	5	0.0033946704810290385
1105	6	-0.012877519481925503
1105	7	0.030407522440960122
1105	8	0.019822348655999633
1105	9	0.016785136314510396
1105	10-11	-0.006011049372723676
1105	12-13	0.040745321890071295
1105	14-15	0.03341073763389346
1105	16-17	0.008745472729607684
1105	18-19	-0.0063211805614429295
1105	20-21	-0.0263648971819066
1105	22-23	-0.014559583644981444
1105	24-25	-0.049488931707870165
1105	26-27	-0.05169092300446465
1105	28-29	-0.038127329879380056
1105	30-31	-0.014282458733134717
1105	32-33	-0.039296307256435625
1105	34-35	0.0055933363716604845
1105	36-37	-0.03170487700869984
1105	38-39	-0.11991314670184039
1105	40-41	-0.0726740978919409
1105	42-43	-0.04504148695613708
1105	44-45	-0.045526691684912635
1105	46-47	-0.10140582236178375
1105	48-49	-0.08436068625459825
1105	50-51	-0.06139296778147241
1105	52-53	-0.12864411680846644
1105	54-55	-0.0809709786461994
1105	56-57	-0.061528710634412676
1105	58-59	-0.13481319149867232
1105	60-61	-0.11304440943121818
1105	62-63	-0.1843197578426512
1105	64-65	-0.08907534351440205
1105	66-67	-0.13799382924178616
1105	68-69	-0.19730860237743286
1105	70-71	-0.05462037085268179
1105	72-73	-0.11558259920121117
1105	74-75	-0.10674775895513733
1105	76-77	-0.11941477434235992
1105	78-79	-0.02387522641459583
1105	80-81	-0.021362278836377868
1105	82-83	0.025241719539685903
1105	84-85	0.015682202278945567
1105	86-87	0.008551376787032439
1105	88-89	0.041334852288976265
1105	90-91	-0.013017883090519433
1105	92-93	-0.010917061140105488
1105	94-95	0.09731299506395175
1105	96-97	0.04093106365052179
1105	98-99	-0.05141463172975236
1105	100	-0.062174194844143216
1106	1	0.028523325703986302
1106	2	-0.008832096623223151
1106	3	0.0036445373679327986
1106	4	-0.002553724682172742
1106	5	-0.020628225208454865
1106	6	-0.010832123613916167
1106	7	-0.036563742589827086
1106	8	-0.014956247609717366
1106	9	0.0333133741484275
1106	10-11	0.014458839609552854
1106	12-13	8.557078977702304E-4
1106	14-15	-0.0014149628874093878
1106	16-17	0.010711766407467849
1106	18-19	0.03713956772330107
1106	20-21	0.048936130597247995
1106	22-23	0.032667033143361834
1106	24-25	0.0012420768999845677
1106	26-27	0.03743019123777458
1106	28-29	0.058628107022890674
1106	30-31	0.017178240112741605
1106	32-33	0.001291630758203155
1106	34-35	0.03848377983831597
1106	36-37	-0.0038074846343718605
1106	38-39	0.03108922636386069
1106	40-41	0.06801862305859885
1106	42-43	0.0553369950508511
1106	44-45	0.0898970818191529
1106	46-47	0.15327974643240339
1106	48-49	0.09292503744804037
1106	50-51	0.019371741655312746
1106	52-53	0.019803502709997645
1106	54-55	0.09586759795383415
1106	56-57	0.08588865778953192
1106	58-59	0.007673473739764347
1106	60-61	0.01584588022561917
1106	62-63	0.12576055285317977
1106	64-65	0.10750966662056527
1106	66-67	0.0809225806174183
1106	68-69	0.1445687288025681
1106	70-71	0.0965105432719966
1106	72-73	0.09645260676661138
1106	74-75	0.11563788619463367
1106	76-77	0.07228814749478829
1106	78-79	0.14238562953702427
1106	80-81	0.21810325952544218
1106	82-83	0.16136052114150345
1106	84-85	0.12564083842535467
1106	86-87	0.13446585853036197
1106	88-89	0.09812830218817936
1106	90-91	0.11825989194066011
1106	92-93	0.11744684189379484
1106	94-95	0.1599481966372025
1106	96-97	0.1432636169483743
1106	98-99	0.10919879476838545
1106	100	0.12537297807654113
1107	1	-0.014349399546027541
1107	2	-0.019262267513028064
1107	3	0.011565073869100218
1107	4	0.014166654678945179
1107	5	-0.009568103494444813
1107	6	0.0048895299026980865
1107	7	-0.03768814570968004
1107	8	0.025001825199666428
1107	9	-0.028681504893718568
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2316	32-33	-0.15525349907214547
2316	34-35	-0.1618782046764622
2316	36-37	-0.19392290843041593
2316	38-39	-0.19573258386714798
2316	40-41	-0.21436024921184327
2316	42-43	-0.22361195613649443
2316	44-45	-0.2480811554147948
2316	46-47	-0.18285363350911155
2316	48-49	-0.16554604277372675
2316	50-51	-0.20633575600785292
2316	52-53	-0.20311375833951928
2316	54-55	-0.19712376068329007
2316	56-57	-0.31570623741487935
2316	58-59	-0.26292927117381737
2316	60-61	-0.15039319491037162
2316	62-63	-0.18882930798198316
2316	64-65	-0.1950749670725216
2316	66-67	-0.21482330882350453
2316	68-69	-0.14447654882027905
2316	70-71	-0.17891237887262434
2316	72-73	-0.15820979662207435
2316	74-75	-0.18601901759973316
2316	76-77	-0.19526024062457736
2316	78-79	-0.17686382877135287
2316	80-81	-0.15769490562004052
2316	82-83	-0.1609070312520089
2316	84-85	-0.17812215124973818
2316	86-87	-0.24359346751873545
2316	88-89	-0.20600864533563623
2316	90-91	-0.3701330853027258
2316	92-93	-0.30120506351558163
2316	94-95	-0.2659038378014458
2316	96-97	-0.2703816142376496
2316	98-99	-0.19713045092442982
2316	100	-0.40369189383234527
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	46.0
14	273.0
15	917.0
16	2382.0
17	5595.0
18	10561.0
19	16320.0
20	24166.0
21	36050.0
22	46590.0
23	47173.0
24	43228.0
25	42364.0
26	47643.0
27	57030.0
28	70387.0
29	87563.0
30	108244.0
31	135074.0
32	173018.0
33	229181.0
34	343014.0
35	509726.0
36	879991.0
37	1583384.0
38	1663689.0
39	399345.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37660018007621	21.154977534662535	13.940287777567331	23.528134507693924
2	15.134594846778594	21.13743493620925	40.5544773932143	23.173492823797858
3	17.826758772932923	27.325510791636564	30.40264062206927	24.44508981336124
4	12.231977944185772	17.93924289949727	39.326977080249755	30.501802076067204
5	12.736860647296947	38.62986608689769	35.24519345492513	13.388079810880239
6	29.46862818234343	38.68366537215409	18.24243404933775	13.605272396164732
7	26.01820961859755	33.04501056298058	23.461122669992374	17.475657148429494
8	25.50199943406637	35.621377297162546	20.37883725172024	18.497786017050846
9	26.284356547159582	15.35602392437377	21.270501632329292	37.08911789613735
10-11	24.83772663628102	26.372845113111204	28.40456758351057	20.384860667097207
12-13	25.458711594938567	24.260540034268953	28.454551888605412	21.82619648218707
14-15	22.83182115092859	25.485663560022804	26.303936397679667	25.378578891368942
16-17	21.665221969613977	28.120861796201574	27.283922814933163	22.92999341925129
18-19	21.28996269556715	27.64897203683605	29.603154154325434	21.45791111327136
20-21	22.57706894267521	26.819505236003554	29.15403823683998	21.449387584481254
22-23	22.69799105726294	26.579238895992287	28.801362247131813	21.921407799612965
24-25	22.220528389087743	26.74198103968392	29.27790826895561	21.759582302272726
26-27	22.122470423388485	26.947483725321337	28.79697045051041	22.13307540077977
28-29	21.624904732142287	27.28758657909388	29.107724520552413	21.97978416821142
30-31	22.4657398157284	26.820525232800275	28.953429736253018	21.760305215218313
32-33	22.491662524117256	27.13709267311571	28.427975826557645	21.94326897620939
34-35	22.485082365819	27.096384326892515	28.935563073076825	21.482970234211656
36-37	22.335577135374297	26.86355512775836	28.74493415101041	22.05593358585693
38-39	22.328766006613257	26.886798238526886	29.025437109493414	21.758998645366443
40-41	22.212406889890943	26.396404982795996	29.324540827198625	22.06664730011444
42-43	22.09142315738212	26.614903373136833	28.781090088823056	22.51258338065799
44-45	22.15280540064108	27.164332023104897	28.645662429762798	22.037200146491223
46-47	22.193828730103395	26.938806886194698	28.136382114812168	22.73098226888974
48-49	22.602774429400895	26.830270295475035	28.269797643919404	22.29715763120467
50-51	22.492578419161376	26.93732135559315	27.91450759437773	22.65559263086774
52-53	22.063239943127506	27.308006962324182	27.86579134268541	22.7629617518629
54-55	22.01839629322257	27.23175869041663	27.8650945607809	22.8847504555799
56-57	22.565380383530787	26.955446121608816	27.610068959233185	22.869104535627212
58-59	22.018906823553074	27.18599481007084	28.09411051227313	22.700987854102955
60-61	22.26272211385511	27.485783387572766	27.633667546962172	22.617826951609956
62-63	22.325429376936324	27.04154176861357	27.563250888238006	23.0697779662121
64-65	21.745607909458002	27.053760111609588	27.79641459073513	23.404217388197278
66-67	22.141041236800017	26.939954877762894	27.606617531938728	23.31238635349836
68-69	21.863278656426857	27.004832813305303	27.71366066847251	23.41822786179533
70-71	21.91461435469747	26.642422240347724	27.844668265455898	23.598295139498905
72-73	22.152719594024468	26.94872347141476	27.731149930557898	23.167407004002875
74-75	22.254366616762475	27.175559898363066	27.468272743549598	23.10180074132486
76-77	21.965077908337566	27.078521337320755	27.317367252145175	23.639033502196508
78-79	21.832374268054195	27.074134370923343	27.48323702302054	23.610254338001923
80-81	21.64777868083357	27.44970898544577	27.522847362340624	23.379664971380038
82-83	22.180611923821512	26.90174614433442	28.02860693200787	22.8890349998362
84-85	22.30788165761978	27.145347424679535	27.194906002880874	23.35186491481981
86-87	21.45487319514113	27.596155018254294	27.543258711167727	23.405713075436854
88-89	21.891680511859736	26.850969244777733	27.495372576888055	23.76197766647448
90-91	21.90623467014436	26.850284768198296	27.538212694726067	23.705267866931273
92-93	21.9596118011961	27.009717464230405	27.384038638563098	23.646632096010404
94-95	21.31666027469772	26.95810997630172	27.67743122995653	24.04779851904403
96-97	21.486868276854263	26.896095086334494	27.48478507113099	24.132251565680253
98-99	21.115820855405975	27.2170043253158	27.356826287534535	24.310348531743692
100	21.196010899753837	27.122889178319216	26.990908542386478	24.690191379540472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	701.0
1	493.5
2	380.5
3	497.5
4	454.0
5	467.0
6	745.5
7	1468.5
8	2461.5
9	3139.5
10	3112.0
11	2923.0
12	3213.0
13	3666.5
14	4246.0
15	5056.0
16	5883.0
17	6675.0
18	7538.0
19	8595.0
20	9771.5
21	11046.5
22	12603.0
23	14619.0
24	17431.0
25	20809.5
26	25448.5
27	31325.5
28	38338.5
29	49300.5
30	64026.5
31	78734.0
32	97886.0
33	118807.5
34	137726.0
35	155903.0
36	175960.5
37	204408.5
38	217824.5
39	213605.5
40	206856.0
41	200851.0
42	202179.0
43	209376.5
44	220286.0
45	243032.0
46	264897.0
47	278346.5
48	286993.0
49	283845.5
50	275003.0
51	269582.0
52	261748.5
53	247868.5
54	235488.5
55	211091.0
56	184823.5
57	155250.0
58	125352.0
59	110393.5
60	89473.0
61	66181.0
62	50344.5
63	39092.0
64	28171.0
65	17299.5
66	11270.5
67	8134.0
68	6243.5
69	4692.5
70	3515.5
71	2607.5
72	1894.0
73	1296.0
74	963.5
75	695.0
76	441.0
77	256.0
78	137.5
79	79.5
80	44.5
81	20.5
82	11.0
83	8.0
84	4.0
85	2.0
86	1.5
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004738623038423376
2	0.03088485176490091
3	0.0013560689724105481
4	0.0015541464627626506
5	0.007222210032838201
6	0.0
7	0.0011122812919771912
8	0.007328867143027795
9	0.0011275180220042759
10-11	1.7522239531147534E-4
12-13	6.856528512188165E-5
14-15	8.684936115438344E-4
16-17	7.618365013542405E-5
18-19	9.294405316521736E-4
20-21	2.971162355281538E-4
22-23	0.001653185207938702
24-25	9.827690867469702E-4
26-27	0.002011248363575195
28-29	2.1331422037918735E-4
30-31	0.0017141321280470412
32-33	0.0015312913677220236
34-35	7.618365013542406E-4
36-37	0.004098680377285814
38-39	8.913487065844614E-4
40-41	0.0034511193511347097
42-43	0.004037733457177475
44-45	0.0036187233814326423
46-47	0.0024378768043335696
48-49	0.00946962771183321
50-51	0.00716126311272986
52-53	0.005134778019127582
54-55	0.004548163913084816
56-57	0.0034282642560940824
58-59	0.0057594839502380585
60-61	0.007907862884057017
62-63	0.003588249921378473
64-65	0.003214950035714895
66-67	0.0037863274117305758
68-69	0.0021407605688054158
70-71	0.0031235296555523866
72-73	0.006498465356551671
74-75	0.00781644250389451
76-77	0.004258666042570205
78-79	0.0028568868800784024
80-81	0.0028340317850377747
82-83	0.00522619839929009
84-85	0.0022474176789950098
86-87	0.003778709046717033
88-89	6.094692010833924E-4
90-91	0.0018055525082095502
92-93	1.1427547520313608E-4
94-95	0.002514060454468994
96-97	0.0019198279834126864
98-99	0.0015084362726813963
100	6.0946920108339247E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6563088.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.29737835461546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9834814879622	44.0071259691673
2	16.28345006801446	19.636986993391133
3	5.235972154441605	9.471461821517895
4	2.1875823863703188	5.276219313314548
5	1.1007424945258073	3.3185943331712924
6	0.6246043107550164	2.259720146851142
7	0.3841394950801728	1.6213823133060086
8	0.25229505517757583	1.21701843192327
9	0.1778796668033287	0.9653109813749918
>10	0.7188585595507638	7.266675979160208
>50	0.03260049512218465	1.3652442790580355
>100	0.016732082093720946	1.855063437036316
>500	0.001182396804254052	0.48272729869165987
>1k	3.8347783881773046E-4	0.3644909590837859
>5k	0.0	0.0
>10k+	9.586945969346769E-5	0.8919777429523443
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19455	0.296430582676935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16743	0.255108570843481	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10093	0.15378431616336702	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	3.0473460054169623E-5
10-11	0.0	0.0	0.0	0.0	3.0473460054169623E-5
12-13	0.0	0.0	0.0	0.0	3.0473460054169623E-5
14-15	0.0	0.0	0.0	0.0	4.5710190081254435E-5
16-17	0.0	0.0	0.0	0.0	6.0946920108339247E-5
18-19	0.0	0.0	0.0	0.0	6.0946920108339247E-5
20-21	0.0	0.0	0.0	2.2855095040627217E-5	7.618365013542405E-5
22-23	0.0	0.0	0.0	1.5998566528439053E-4	1.1427547520313609E-4
24-25	0.0	0.0	0.0	3.352080605958658E-4	1.371305702437633E-4
26-27	0.0	0.0	0.0	0.0012113200371532424	1.371305702437633E-4
28-29	0.0	0.0	0.0	0.003573013191351388	1.371305702437633E-4
30-31	0.0	0.0	0.0	0.01305025926819814	1.371305702437633E-4
32-33	0.0	0.0	0.0	0.038983173774296484	1.371305702437633E-4
34-35	0.0	0.0	0.0	0.0820650279258788	1.8284076032501774E-4
36-37	0.0	0.0	0.0	0.15165117395957511	1.8284076032501774E-4
38-39	0.0	0.0	0.0	0.2518022004276036	1.9045912533856014E-4
40-41	0.0	0.0	0.0	0.36006678563505473	1.9807749035210254E-4
42-43	0.0	0.0	0.0	0.47194247585892496	1.9807749035210254E-4
44-45	0.0	0.0	0.0	0.6121889573932271	1.9807749035210254E-4
46-47	0.0	7.618365013542406E-6	0.0	0.7797244224060382	2.1331422037918735E-4
48-49	0.0	1.5236730027084812E-5	0.0	0.963677464023033	2.1331422037918735E-4
50-51	0.0	1.5236730027084812E-5	0.0	1.1569096742265226	2.1331422037918735E-4
52-53	0.0	1.5236730027084812E-5	0.0	1.3465612528736473	2.2855095040627218E-4
54-55	0.0	1.5236730027084812E-5	0.0	1.5586108246605868	2.4378768043335699E-4
56-57	0.0	1.5236730027084812E-5	0.0	1.804973512468521	2.4378768043335699E-4
58-59	0.0	1.5236730027084812E-5	0.0	2.0894051702491265	2.4378768043335699E-4
60-61	0.0	1.5236730027084812E-5	0.0	2.3875575034191225	2.4378768043335699E-4
62-63	0.0	1.5236730027084812E-5	0.0	2.6883457908838038	2.4378768043335699E-4
64-65	0.0	1.5236730027084812E-5	0.0	2.996226776176093	2.4378768043335699E-4
66-67	0.0	1.5236730027084812E-5	0.0	3.336196314905423	2.4378768043335699E-4
68-69	0.0	1.5236730027084812E-5	0.0	3.7222341068716434	2.4378768043335699E-4
70-71	0.0	1.5236730027084812E-5	0.0	4.147110323676904	2.4378768043335699E-4
72-73	0.0	1.5236730027084812E-5	0.0	4.577646985687226	2.590244104604418E-4
74-75	0.0	1.5236730027084812E-5	0.0	5.000161509338287	2.590244104604418E-4
76-77	0.0	1.5236730027084812E-5	0.0	5.461186868132806	2.6664277547398417E-4
78-79	0.0	1.5236730027084812E-5	0.0	5.964608428227688	2.742611404875266E-4
80-81	0.0	1.5236730027084812E-5	0.0	6.498709144232105	2.894978705146114E-4
82-83	0.0	1.5236730027084812E-5	0.0	7.044176460836728	3.3520806059586587E-4
84-85	0.0	1.5236730027084812E-5	0.0	7.583510993605449	3.3520806059586587E-4
86-87	0.0	1.5236730027084812E-5	0.0	8.140649645410818	3.3520806059586587E-4
88	0.0	1.5236730027084812E-5	0.0	8.577532405477422	3.504447906229507E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11595	0.0	53.80977	1
GTATCAA	21285	0.0	41.21914	1
CAACGCA	25490	0.0	33.1547	5
ATCAACG	25560	0.0	33.083046	3
TATCAAC	27020	0.0	32.21132	2
AACGCAG	27950	0.0	30.63788	6
TCAACGC	28270	0.0	30.094326	4
GTACTAG	3895	0.0	29.333221	1
GTGGTAT	4080	0.0	28.118402	1
ACGCAGA	30420	0.0	27.763933	7
CGCAGAG	30885	0.0	27.284433	8
CTGTGCG	5300	0.0	24.915693	9
GCAGAGT	33620	0.0	24.755028	9
GTACATG	26915	0.0	24.316765	1
TACATGG	26720	0.0	24.126173	2
TAGTACT	5710	0.0	24.03691	4
ACATGGG	26625	0.0	23.479952	3
GGGCACC	5260	0.0	22.873703	94
TGGTATC	4940	0.0	22.843979	2
GAGTACT	19190	0.0	22.273243	12-13
>>END_MODULE