FastQCFastQC Report
Thu 26 May 2016
ERR580214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593060
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161590.24509105028621006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121300.18398133795233168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70410.10679411381058265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105800.048.004391
GTATCAA192250.042.3177531
CAACGCA219000.035.9926725
ATCAACG227950.034.5794943
TATCAAC241400.033.6740262
GTACTAG49850.033.5836331
AACGCAG247100.032.6648836
TCAACGC265400.030.0013624
GTGGTAT31600.029.912441
ACGCAGA265200.029.5683217
CGCAGAG271050.028.791278
TAGGCAT63750.028.681025
GCATCGT52550.028.6099998
TAGTACT66350.027.6990154
CTAGGCA67350.027.6366944
CTGTGCG68350.026.9488899
GCAGAGT292350.026.1503649
GTACATG280100.026.0736121
GGGCACC62800.025.75075394
TACATGG284450.025.3545992
Produced by FastQC (version 0.11.5)