FastQCFastQC Report
Thu 26 May 2016
ERR580214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593060
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136220.2066111941951082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114190.17319727106988259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108350.047.349481
GTATCAA189900.039.4961851
GTGGTAT30350.033.621761
CAACGCA219600.032.982645
GTACTAG50300.032.813951
ATCAACG224600.032.2084853
TATCAAC238600.031.3164752
AACGCAG249900.029.6026446
TCAACGC258950.028.3342674
TAGTACT67650.027.6528724
TAGGCAT67800.027.1751175
ACGCAGA267350.026.9497037
GGGCACC63000.026.7801594
CTAGGCA70450.026.5538234
CTGTGCG70150.026.5291489
CGCAGAG274450.026.0639468
GTACATG282600.025.0262071
GTACTGT81750.024.4914176
CTAGTAC80100.024.4706213
TACATGG287950.024.3162732
Produced by FastQC (version 0.11.5)