FastQCFastQC Report
Thu 26 May 2016
ERR580213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7899389
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104220.1319342546619745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.10772985100493215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107050.048.0552981
GTATCAA188000.040.644911
CAACGCA220550.033.6614955
ATCAACG227300.032.7262043
TATCAAC238450.031.8285482
AACGCAG244850.030.7827036
TCAACGC247150.030.135454
ACGCAGA274100.026.725677
CGCAGAG282450.025.7520988
GTACATG332150.024.4352721
GTACTAG37050.024.2412931
TACATGG337700.023.741272
ACATGGG341750.022.7983063
GCAGAGT313900.022.4883799
GTGGTAT50400.021.925421
CATGGGG263200.020.3658284
GAGTACT117300.019.37841612-13
AGAGTAC280600.019.19612710-11
CAGAGTA308050.018.91982510-11
ATGGGGG179300.018.3034735
Produced by FastQC (version 0.11.5)