FastQCFastQC Report
Thu 26 May 2016
ERR580213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7899389
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90910.11508485023335349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108600.046.0326841
GTATCAA187250.040.0717771
CAACGCA211800.033.9050755
ATCAACG218350.032.8898743
TATCAAC233100.031.6950442
AACGCAG237150.030.7948956
TCAACGC235900.030.5813054
ACGCAGA266500.026.8214867
CGCAGAG278700.025.5632368
GTACATG328750.023.828361
GTACTAG36300.023.5159341
TACATGG331100.023.2506872
GCAGAGT315000.022.0206139
ACATGGG338800.021.959863
CGTTAAC13350.021.196311
TAGTACT57550.021.1509554
GTGGTAT50950.020.9196551
CTAGTAC56400.020.8330573
CATGGGG262950.020.0537344
GAGTACT118300.018.90961512-13
Produced by FastQC (version 0.11.5)