FastQCFastQC Report
Thu 26 May 2016
ERR580212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7278927
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218260.29985188751034325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178330.24499490103417715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107080.14710959458722417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109400.051.6795961
GTATCAA192700.040.88511
CAACGCA226550.033.3395465
ATCAACG233600.032.438493
TATCAAC246050.031.6578122
AACGCAG252150.030.533066
TCAACGC253750.029.9906944
GTACATG256750.028.5056611
TACATGG259450.027.7202852
GTACTAG39300.027.6472131
ACGCAGA274750.027.576547
GTGGTAT40650.027.1918851
ACATGGG258750.026.7041873
CGCAGAG284200.026.6261438
CATGGGG210050.025.2133034
ATGGGGG151150.024.0207925
TAGTACT61950.023.8397874
CTGTGCG62250.023.7844229
GCAGAGT314800.023.6505839
CTAACGC18700.023.6441763
Produced by FastQC (version 0.11.5)