FastQCFastQC Report
Thu 26 May 2016
ERR580212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7278927
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190610.2618655194646134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158360.21755953865178207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107080.14710959458722417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.047.5485081
GTATCAA181800.040.0280721
CAACGCA218550.032.215295
ATCAACG222700.031.5945073
TATCAAC235200.030.8558772
AACGCAG244350.029.4471346
TCAACGC243650.029.0898824
GTACATG238900.027.1353661
GTACTAG40900.027.0104711
TACATGG240600.026.5101472
ACGCAGA273200.025.804227
TAGTACT58100.025.7251574
ACATGGG241050.025.309153
CTGTGCG57850.025.1848019
CGCAGAG282850.024.8241628
GTGGTAT39800.024.6860051
CTAGTAC60600.023.7333373
CATGGGG194050.023.1069054
GCAGAGT312800.022.3270979
ATGGGGG140800.021.9980325
Produced by FastQC (version 0.11.5)