FastQCFastQC Report
Thu 26 May 2016
ERR580211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8158324
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222190.27234760472861824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173910.21316878319615645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110150.13501547621790946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111400.047.7471051
GTATCAA200150.040.720451
CAACGCA227550.034.368335
ATCAACG236850.033.001823
TATCAAC256250.031.4208182
AACGCAG260000.030.8955466
TCAACGC265750.029.677464
GTACTAG55250.029.5370311
GTACATG282350.028.3492371
ACGCAGA281700.027.79767
TACATGG287000.027.169412
CGCAGAG291650.026.7841328
GTGGTAT43900.026.4607451
ACATGGG287500.026.0590273
CATGGGG235700.024.721724
CTAACGC23750.024.554913
CTGTGCG74900.024.5365859
TAGTACT75800.024.3211334
GCATCGT58450.024.2041878
TAACGCC24050.023.8567734
Produced by FastQC (version 0.11.5)