FastQCFastQC Report
Thu 26 May 2016
ERR580211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8158324
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191200.23436186157843203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156910.1923311699805009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106210.13018605291969282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113600.047.25831
GTATCAA201300.040.3543431
CAACGCA236000.033.437865
ATCAACG243850.032.3809053
TATCAAC262950.030.7808822
GTACTAG54550.030.593131
AACGCAG265950.030.3780386
TCAACGC272350.029.1132164
GTACATG273000.027.3450151
ACGCAGA293950.026.8926567
TACATGG274500.026.7803062
ACATGGG273800.025.9378223
CGCAGAG304350.025.9273768
TAGGCAT70350.025.7883175
TAGTACT79050.024.2583244
CTGTGCG76000.024.2413239
GGGCACC69250.024.16569394
GTGGTAT41800.024.0673261
CTAGGCA77550.024.0002564
CATGGGG226350.023.6300284
Produced by FastQC (version 0.11.5)