FastQCFastQC Report
Thu 26 May 2016
ERR580210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7375905
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191160.2591681969873527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157750.21387206044546397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87770.118995567323603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114850.052.2935181
GTATCAA215900.043.437061
CAACGCA255300.035.6684275
ATCAACG257650.035.39763
TATCAAC272100.034.3706742
AACGCAG279950.033.0436066
TCAACGC286900.032.002414
GTACTAG45600.030.9417131
ACGCAGA302500.030.0379987
CGCAGAG310400.029.1972248
GTGGTAT39350.028.2068921
GTACATG303950.026.1191021
GCAGAGT342250.026.0031819
TAGGCAT56650.025.8916555
TACATGG307900.025.2737752
GCATCGT47950.024.6912448
ACATGGG308350.024.5614363
CTAGGCA64100.024.42294
GAGTACT175450.022.5523312-13
CATGGGG245600.022.070494
Produced by FastQC (version 0.11.5)