FastQCFastQC Report
Thu 26 May 2016
ERR580210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7375905
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167240.22673827821806272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147360.19978565342151236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81150.11002039749698511No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113400.051.1256941
GTATCAA202800.041.5952451
CAACGCA243600.033.3230865
ATCAACG247400.032.792923
TATCAAC258050.032.4721262
AACGCAG270200.030.8048576
TCAACGC272200.030.0465244
GTACTAG41150.028.5666521
GTGGTAT40900.027.5916161
ACGCAGA296500.027.327237
CGCAGAG306300.026.3301498
GTACATG294650.025.5968861
TACATGG299900.024.7421472
ACATGGG296750.024.0922573
GCAGAGT336050.023.521289
TAGGCAT54200.022.8946915
GAGTACT171150.022.2266712-13
CATGGGG239000.022.0268944
CTAGGCA60800.021.5691854
TAGTACT58700.021.540084
Produced by FastQC (version 0.11.5)