##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR580210_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7375905 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.547829588369154 34.0 31.0 34.0 31.0 34.0 2 32.66330111355827 34.0 31.0 34.0 31.0 34.0 3 32.7150530002759 34.0 31.0 34.0 31.0 34.0 4 36.23057699360282 37.0 35.0 37.0 35.0 37.0 5 36.174727575802564 37.0 35.0 37.0 35.0 37.0 6 36.211091384718216 37.0 37.0 37.0 35.0 37.0 7 36.15659475006796 37.0 36.0 37.0 35.0 37.0 8 36.15111027053629 37.0 36.0 37.0 35.0 37.0 9 37.87163121000067 39.0 38.0 39.0 35.0 39.0 10-11 37.82296463959338 39.0 38.0 39.0 35.0 39.0 12-13 37.78908479705203 39.0 38.0 39.0 35.0 39.0 14-15 39.18169824584237 41.0 39.0 41.0 36.0 41.0 16-17 39.17312845000038 40.5 39.0 41.0 36.0 41.0 18-19 39.15980805067311 40.0 39.0 41.0 36.0 41.0 20-21 39.12874704866725 40.0 39.0 41.0 35.5 41.0 22-23 39.05441149255583 40.0 39.0 41.0 35.0 41.0 24-25 38.98705874058844 40.0 38.0 41.0 35.0 41.0 26-27 38.86822383151627 40.0 38.0 41.0 35.0 41.0 28-29 38.768351612446196 40.0 38.0 41.0 35.0 41.0 30-31 38.621009015164915 40.0 38.0 41.0 34.5 41.0 32-33 38.52101111660196 40.0 38.0 41.0 34.0 41.0 34-35 38.43392451502561 40.0 38.0 41.0 34.0 41.0 36-37 38.31764739377744 40.0 38.0 41.0 34.0 41.0 38-39 38.2028943566925 40.0 38.0 41.0 34.0 41.0 40-41 38.091263038230565 40.0 37.5 41.0 33.0 41.0 42-43 38.09730243271842 40.0 38.0 41.0 33.5 41.0 44-45 38.06423157565072 40.0 37.5 41.0 33.0 41.0 46-47 37.88775472297976 40.0 37.0 41.0 33.0 41.0 48-49 37.691509041941295 40.0 37.0 41.0 33.0 41.0 50-51 37.5013355649239 40.0 36.5 41.0 33.0 41.0 52-53 37.32726356969077 40.0 36.0 41.0 32.0 41.0 54-55 37.109569334203734 39.0 35.5 41.0 32.0 41.0 56-57 36.86888219140566 39.0 35.0 41.0 31.5 41.0 58-59 36.622536082012985 39.0 35.0 41.0 31.0 41.0 60-61 36.35975951425622 38.0 35.0 40.0 31.0 41.0 62-63 36.05730178195083 37.5 35.0 40.0 31.0 41.0 64-65 35.75249708340874 37.0 35.0 40.0 30.0 41.0 66-67 35.42620491993864 36.5 34.5 39.5 30.0 41.0 68-69 35.10185434872059 36.0 34.0 39.0 29.5 41.0 70-71 34.778770198368875 35.5 34.0 39.0 29.0 40.5 72-73 34.4640076031348 35.0 34.0 38.0 29.0 40.0 74-75 34.11543220797991 35.0 34.0 37.0 29.0 39.5 76-77 32.59852648590241 34.0 31.5 35.5 26.5 38.5 78-79 33.48886150241903 35.0 33.0 36.0 28.5 39.0 80-81 33.47313143268521 35.0 34.0 36.0 29.0 37.5 82-83 33.25834056973348 35.0 33.0 35.5 29.0 37.0 84-85 33.05644825956951 35.0 33.0 35.0 29.0 37.0 86-87 32.86690189203901 35.0 33.0 35.0 28.0 36.0 88-89 32.69811196049841 35.0 33.0 35.0 27.0 36.0 90-91 32.542769327967214 35.0 33.0 35.0 27.0 36.0 92-93 32.39559132065828 35.0 33.0 35.0 27.0 35.5 94-95 32.27048694363607 35.0 33.0 35.0 26.0 35.0 96-97 32.152102691127396 35.0 33.0 35.0 25.5 35.0 98-99 32.053934669711715 35.0 32.0 35.0 25.0 35.0 100 30.195383075026047 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03429351417074855 1101 2 -0.0552966915991675 1101 3 -0.05962314212207076 1101 4 -0.039824405945068975 1101 5 -0.056091185461148996 1101 6 -0.07282216185092949 1101 7 -0.05157892922809992 1101 8 -0.046652065148357735 1101 9 -0.07387384271001451 1101 10-11 -0.05556245883890654 1101 12-13 -0.04508001369895709 1101 14-15 -0.13092780528327097 1101 16-17 -0.06383582818079248 1101 18-19 -0.07219966542282208 1101 20-21 -0.13747611928038594 1101 22-23 -0.12120551632335719 1101 24-25 -0.2039076805544866 1101 26-27 -0.2462764518852154 1101 28-29 -0.16636379254845934 1101 30-31 -0.17195515364372937 1101 32-33 -0.13257290027083712 1101 34-35 -0.1911629948549205 1101 36-37 -0.1711778814195526 1101 38-39 -0.18770561684061704 1101 40-41 -0.21084729928247725 1101 42-43 -0.10253241689961357 1101 44-45 -0.12691171739815132 1101 46-47 -0.17508044617766672 1101 48-49 -0.13706820304409462 1101 50-51 -0.1091948931184561 1101 52-53 -0.07312149727997053 1101 54-55 -0.06112744694383565 1101 56-57 -0.10884917709803688 1101 58-59 -0.16240812904165125 1101 60-61 -0.13993907271083117 1101 62-63 -0.18674234584592853 1101 64-65 -0.198495786681832 1101 66-67 -0.20796880738416945 1101 68-69 -0.18537736497458468 1101 70-71 -0.1922260175738586 1101 72-73 -0.17315267247335697 1101 74-75 -0.1765680663903595 1101 76-77 -0.17366672327236188 1101 78-79 -0.1302342336205129 1101 80-81 -0.0534554179581761 1101 82-83 -0.10671387118802755 1101 84-85 -0.1452830237356224 1101 86-87 -0.18953751301456379 1101 88-89 -0.16285198162213987 1101 90-91 -0.1482738783388129 1101 92-93 -0.1689756112645142 1101 94-95 -0.26649040742425 1101 96-97 -0.28799772023363346 1101 98-99 -0.3513618547224233 1101 100 -0.43494522488158793 1102 1 -0.055048421149557214 1102 2 -0.07839031337527302 1102 3 -0.08219795662715512 1102 4 -0.06243212515405361 1102 5 -0.028301550487988436 1102 6 -0.0566785818710116 1102 7 -0.029414961186937205 1102 8 -0.056858229243601954 1102 9 -0.10623926128705108 1102 10-11 -0.018899086025562895 1102 12-13 -0.02406676307167288 1102 14-15 -0.04119796327659486 1102 16-17 -0.08289534228048012 1102 18-19 -0.05559496853006607 1102 20-21 -0.10556134178688836 1102 22-23 -0.11363901313150393 1102 24-25 -0.14665887464679628 1102 26-27 -0.17956894188835548 1102 28-29 -0.13570480650273709 1102 30-31 -0.16956763433702093 1102 32-33 -0.1861269522235105 1102 34-35 -0.18089202495663415 1102 36-37 -0.2000256428115108 1102 38-39 -0.3075148349614807 1102 40-41 -0.23734938830266827 1102 42-43 -0.114513360136975 1102 44-45 -0.12310294717818948 1102 46-47 -0.13921341681501787 1102 48-49 -0.17469794573901254 1102 50-51 -0.14397683405169914 1102 52-53 -0.1759860558579618 1102 54-55 -0.2430264317581674 1102 56-57 -0.22536948098053244 1102 58-59 -0.1277968798503224 1102 60-61 -0.20900009221763582 1102 62-63 -0.1449132164324496 1102 64-65 -0.18319955357129203 1102 66-67 -0.1446515193226574 1102 68-69 -0.16675241172959687 1102 70-71 -0.1480498062313913 1102 72-73 -0.21342459072174336 1102 74-75 -0.2398278670699483 1102 76-77 -0.21054142818156407 1102 78-79 -0.19866248597365654 1102 80-81 -0.19447187561005563 1102 82-83 -0.17222406944732427 1102 84-85 -0.256640638214499 1102 86-87 -0.23121757893223105 1102 88-89 -0.19929950048004486 1102 90-91 -0.22513680837075611 1102 92-93 -0.20203155501212677 1102 94-95 -0.25778541049877646 1102 96-97 -0.3122601017561166 1102 98-99 -0.28925679715215935 1102 100 -0.1255146217252836 1103 1 -0.02108963034042688 1103 2 -0.037667095906158465 1103 3 -0.04986886281966463 1103 4 -0.036173082452890526 1103 5 -0.023823719791167264 1103 6 -0.03221138736717677 1103 7 -0.013009599349565804 1103 8 -0.04761146400061733 1103 9 -0.0748381987809239 1103 10-11 -0.05048457396282657 1103 12-13 -0.03554723797451942 1103 14-15 -0.04318149431150431 1103 16-17 -0.08841076383266255 1103 18-19 -0.06551600685648395 1103 20-21 -0.0561707402046423 1103 22-23 -0.08094477901543229 1103 24-25 -0.020727408354936472 1103 26-27 -0.08677835124132827 1103 28-29 -0.1020959645588917 1103 30-31 -0.12388309011360121 1103 32-33 -0.11780375252139663 1103 34-35 -0.06423945293724387 1103 36-37 -0.14467499712606013 1103 38-39 -0.10376924929259701 1103 40-41 -0.0783242358780285 1103 42-43 -2.2056929531544256E-4 1103 44-45 0.01584230392970909 1103 46-47 -0.010973625994203928 1103 48-49 -0.04065252441491651 1103 50-51 -0.07006070587912916 1103 52-53 -0.04632301669404626 1103 54-55 0.02491408981253329 1103 56-57 0.0379621727815973 1103 58-59 -0.027103988518135225 1103 60-61 -0.07312442937906383 1103 62-63 -0.05142691879780159 1103 64-65 -0.12653765278341922 1103 66-67 -0.11063532406546273 1103 68-69 -0.09396091963629516 1103 70-71 -0.08675905521204896 1103 72-73 -0.13494472162134485 1103 74-75 -0.12461834028886187 1103 76-77 -0.13563816177024535 1103 78-79 -0.058591049084661506 1103 80-81 -0.09796030899574504 1103 82-83 -0.0770879723408271 1103 84-85 -0.08045634679984204 1103 86-87 -0.08312341206448082 1103 88-89 -0.116618127797814 1103 90-91 -0.07406958219625182 1103 92-93 -0.1275220874798535 1103 94-95 -0.10016722363463515 1103 96-97 -0.13091387359396123 1103 98-99 -0.17051129824475453 1103 100 -0.009018165795147581 1104 1 0.01409025949439524 1104 2 -0.0022286993421332113 1104 3 -0.01673973316317756 1104 4 0.009510739693894266 1104 5 -0.029503139992549166 1104 6 -0.03395167630613827 1104 7 -0.009366319086517194 1104 8 0.007665762797174125 1104 9 -0.026017959205731245 1104 10-11 -0.004116702115666726 1104 12-13 0.008199277056306187 1104 14-15 -0.03997279023933942 1104 16-17 -0.06625398853461917 1104 18-19 -0.03178698786685885 1104 20-21 -0.03045813456479607 1104 22-23 -0.03210368471219027 1104 24-25 0.02067701732955385 1104 26-27 -0.07251248497571083 1104 28-29 -0.05922095159126428 1104 30-31 -0.05372357640283809 1104 32-33 -0.05434753530506953 1104 34-35 -0.03348443095268294 1104 36-37 0.00959142596183682 1104 38-39 0.014447202149170835 1104 40-41 -0.01305816870943488 1104 42-43 -0.006648326480949152 1104 44-45 -0.07320006429352333 1104 46-47 -0.03001985124560491 1104 48-49 -0.038556660796189135 1104 50-51 -0.047411781973707434 1104 52-53 -0.159487778381731 1104 54-55 -0.11019837774123431 1104 56-57 -0.13943170510987102 1104 58-59 -0.10071522601890592 1104 60-61 -0.19237007261542516 1104 62-63 -0.18706509885278422 1104 64-65 -0.17575435149521468 1104 66-67 -0.18286702704919122 1104 68-69 -0.12577882262561246 1104 70-71 -0.11523718367763536 1104 72-73 -0.14333799254310264 1104 74-75 -0.18898087662564222 1104 76-77 -0.19413614389516454 1104 78-79 -0.09648491495619993 1104 80-81 -0.0646508177928311 1104 82-83 -0.045205312131344044 1104 84-85 -0.09115889507216224 1104 86-87 -0.04853743815045419 1104 88-89 -0.052530566846293425 1104 90-91 -0.08258829477718166 1104 92-93 -0.013085229153048772 1104 94-95 -0.06172529718075026 1104 96-97 -0.1457046586906081 1104 98-99 -0.07391836973319599 1104 100 0.09357669496910148 1105 1 0.014072850329561959 1105 2 -0.00912270825395467 1105 3 0.008028703055842357 1105 4 0.02256810199610726 1105 5 0.02110534289688104 1105 6 5.247007094197897E-4 1105 7 0.00771335439410592 1105 8 0.03002077973058448 1105 9 0.015973353600656992 1105 10-11 -0.015976934906127838 1105 12-13 0.045638693926250085 1105 14-15 0.01227764159447986 1105 16-17 -0.010926278110403587 1105 18-19 -0.024973957023497917 1105 20-21 -0.01593133694279203 1105 22-23 -0.04031196339924037 1105 24-25 -0.03339727394996572 1105 26-27 -0.0897161809393694 1105 28-29 -0.05799516313273756 1105 30-31 -0.067723802783064 1105 32-33 -0.09820970212139457 1105 34-35 -0.11941475523980927 1105 36-37 -0.03537112224144323 1105 38-39 -0.05744923803905522 1105 40-41 -0.06950699880643185 1105 42-43 -0.10183697697604543 1105 44-45 -0.07562211689594989 1105 46-47 -0.056017038535422614 1105 48-49 -0.07305693214500764 1105 50-51 -0.07600513387586005 1105 52-53 -0.09740381542542309 1105 54-55 -0.10755928070310006 1105 56-57 -0.07293941583259311 1105 58-59 -0.15294033820773478 1105 60-61 -0.07050263039455729 1105 62-63 -0.11547703504044193 1105 64-65 -0.076261249362652 1105 66-67 -0.05167783224790412 1105 68-69 -0.06998751937695857 1105 70-71 -0.031178741661058496 1105 72-73 -0.0631034684914411 1105 74-75 -0.08002783793940438 1105 76-77 -0.09138273278311004 1105 78-79 -0.05450757020486918 1105 80-81 -0.03673169942877763 1105 82-83 0.027496541179104383 1105 84-85 -0.016458764882152366 1105 86-87 -0.07038647959674904 1105 88-89 -0.030743455195128888 1105 90-91 0.011730162177066461 1105 92-93 -0.03716601354201288 1105 94-95 -0.025903252808468835 1105 96-97 -0.08142944182586831 1105 98-99 0.010409201682406888 1105 100 0.0013291752507775811 1106 1 0.042608919738100326 1106 2 5.156118818518962E-4 1106 3 0.016264966707375095 1106 4 0.014752755615013768 1106 5 0.03005711200366079 1106 6 -0.02136563482482501 1106 7 0.0046276288280466815 1106 8 -0.00290833453984618 1106 9 0.04415563611387796 1106 10-11 0.03819458260554853 1106 12-13 0.013178404565650226 1106 14-15 -0.007372345211429376 1106 16-17 -0.025777896590632565 1106 18-19 0.048230783362818386 1106 20-21 -0.01881307241566077 1106 22-23 -0.00315060336595252 1106 24-25 -0.031253212164912725 1106 26-27 -0.05028908892576567 1106 28-29 0.006896085755791148 1106 30-31 -0.03358763161820377 1106 32-33 -0.024978394508892166 1106 34-35 0.023835786081860988 1106 36-37 -0.0027260847750127937 1106 38-39 0.03438052364194277 1106 40-41 -0.009451331611842306 1106 42-43 -0.035430510621004885 1106 44-45 -0.0058927739779619515 1106 46-47 -0.008941145846300458 1106 48-49 7.625700048095041E-4 1106 50-51 0.01857360415355913 1106 52-53 0.03429069481728675 1106 54-55 0.029996075144779866 1106 56-57 0.04043336302938627 1106 58-59 -0.006227827417930598 1106 60-61 0.01200427770992718 1106 62-63 0.06886113656339887 1106 64-65 0.0730818211029316 1106 66-67 0.011644294657223497 1106 68-69 0.04306902286120362 1106 70-71 -0.013637499555827048 1106 72-73 -0.021629265816734744 1106 74-75 -0.02298798407762348 1106 76-77 -0.043801639261062064 1106 78-79 0.08942525791351841 1106 80-81 0.08848510501943707 1106 82-83 0.03785939577673503 1106 84-85 0.059736925378565786 1106 86-87 0.10005706159195427 1106 88-89 0.06440167491968651 1106 90-91 0.024451117995020866 1106 92-93 0.06935870661701671 1106 94-95 0.1175346163351918 1106 96-97 0.09083683247238383 1106 98-99 0.10426183624352348 1106 100 0.16472783886924702 1107 1 0.01826191796221366 1107 2 0.040979557329933414 1107 3 -0.0045055112555942856 1107 4 0.003909000351413283 1107 5 -0.003400155197653021 1107 6 9.949946117870923E-5 1107 7 -0.05306850253226969 1107 8 -0.011367728417177148 1107 9 -0.05887958403393867 1107 10-11 -0.020955826170165892 1107 12-13 -0.040677690606564454 1107 14-15 -0.005260108850073664 1107 16-17 0.02498267709915325 1107 18-19 0.004625805474127276 1107 20-21 0.026328336424235488 1107 22-23 -0.008352068404150259 1107 24-25 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-0.2984130624513668 2315 94-95 -0.32995136034618966 2315 96-97 -0.2669463935854779 2315 98-99 -0.24560732038893462 2315 100 -0.16829896232036745 2316 1 -0.1799397276457526 2316 2 -0.13664508093640393 2316 3 -0.08137303679998809 2316 4 -0.0666083533578572 2316 5 -0.12191384334136046 2316 6 -0.09295981491723637 2316 7 -0.12826046503398914 2316 8 -0.12217714633729315 2316 9 -0.09498266518873066 2316 10-11 -0.08105951407210199 2316 12-13 -0.11580770078484903 2316 14-15 -0.23889920442461943 2316 16-17 -0.20001785020816953 2316 18-19 -0.13465093979659315 2316 20-21 -0.08011429778655099 2316 22-23 -0.16518255601604892 2316 24-25 -0.14182960800307143 2316 26-27 -0.15720484594289275 2316 28-29 -0.27979407251471855 2316 30-31 -0.14687040621763003 2316 32-33 -0.14756231974212142 2316 34-35 -0.22275127543556295 2316 36-37 -0.1535586781185856 2316 38-39 -0.15117289204235362 2316 40-41 -0.19189704100880078 2316 42-43 -0.2837803031068944 2316 44-45 -0.2665719901489041 2316 46-47 -0.2444825003131612 2316 48-49 -0.2608958241629935 2316 50-51 -0.1899574418412726 2316 52-53 -0.20767878550617525 2316 54-55 -0.24053903747076077 2316 56-57 -0.4038028925055812 2316 58-59 -0.36947177114757324 2316 60-61 -0.17232307018228 2316 62-63 -0.2347980198827102 2316 64-65 -0.15210361531042338 2316 66-67 -0.24814410108454155 2316 68-69 -0.13661559192871664 2316 70-71 -0.11249897210329607 2316 72-73 -0.15137909507622282 2316 74-75 -0.1362175636443439 2316 76-77 -0.17097732529065013 2316 78-79 -0.13891144521846144 2316 80-81 -0.14209060260903073 2316 82-83 -0.1643347230085226 2316 84-85 -0.168189007816558 2316 86-87 -0.18761391247410586 2316 88-89 -0.17067779525043392 2316 90-91 -0.2062385837641827 2316 92-93 -0.19680001572887562 2316 94-95 -0.14456227336143712 2316 96-97 -0.25449028723100753 2316 98-99 -0.1385437988000433 2316 100 -0.2570907380260863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 11.0 13 53.0 14 219.0 15 736.0 16 2170.0 17 4868.0 18 9218.0 19 14341.0 20 21372.0 21 32224.0 22 42402.0 23 44827.0 24 42355.0 25 42715.0 26 49285.0 27 60794.0 28 74868.0 29 93760.0 30 115600.0 31 145826.0 32 187064.0 33 250288.0 34 372804.0 35 565017.0 36 1007670.0 37 1861453.0 38 1899571.0 39 434277.0 40 116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.336180497499434 20.20307432988508 13.545827104399063 23.914918068216423 2 15.638196286903833 20.597684444735577 39.750672778806006 24.013446489554585 3 18.351778104702635 26.44901172116446 29.658167388689748 25.541042785443157 4 12.782396179510094 17.23283740574592 38.15701276388973 31.827753650854252 5 13.43853812264648 38.01537084193344 34.61053533011732 13.935555705302754 6 30.807671955555815 37.347779654911406 17.79081151148544 14.053736878047337 7 27.071566880093144 32.31442593265962 22.75200580489658 17.862001382350652 8 26.0760342196291 35.05783267004871 19.996566973306997 18.869566137015187 9 26.49739839307489 15.328683187483003 20.656777966291504 37.5171404531506 10-11 25.391083817043945 25.828509558960448 27.8561928979191 20.924213726076502 12-13 25.92982425932902 23.602351253166308 27.955333971944967 22.5124905155597 14-15 23.327406320775886 24.879610240738845 25.56608167075321 26.22690176773206 16-17 22.49661210812322 27.5855643098174 26.494599065510588 23.423224516548796 18-19 22.20410902490734 27.162034141885584 28.508965930987497 22.12489090221958 20-21 23.406963538843208 26.315961442215013 28.08575452686018 22.1913204920816 22-23 23.55383231749706 26.012013511522884 27.794252498227813 22.639901672752238 24-25 23.12507058545833 26.173738725138158 28.10042177056869 22.600768918834824 26-27 22.9330868861295 26.40701086526573 27.729743877623857 22.93015837098091 28-29 22.55693822899697 26.636053855481535 28.037844392431122 22.76916352309037 30-31 23.173056475403207 26.310288887185113 27.91395906745175 22.60269556995993 32-33 23.234273169764798 26.538347704455134 27.45807328763697 22.769305838143104 34-35 23.228011862773986 26.51926799181626 27.882311542139604 22.370408603270146 36-37 23.142885925030882 26.29970834903263 27.681969337532948 22.875436388403543 38-39 23.158041374962504 26.2978271958947 27.92782514528011 22.61630628386268 40-41 22.97578199490686 25.883793301094183 28.247747727899025 22.892676976099935 42-43 22.888529144388833 26.054750762818777 27.80066177622039 23.256058316571995 44-45 22.982725984021265 26.47185851811283 27.67969669785525 22.865718800010658 46-47 22.967071584943426 26.349917777596705 27.27874147185762 23.40426916560225 48-49 23.36276253987512 26.253065504684407 27.3412103487723 23.042961606668175 50-51 23.155173177046525 26.386489871179442 27.101168347894212 23.35716860387982 52-53 22.854888497113908 26.60066389634182 27.091440921889344 23.45300668465493 54-55 22.784934210517395 26.531335675570066 27.09191600216444 23.591814111748093 56-57 23.254514108172643 26.318961003322556 26.847639544913577 23.578885343591228 58-59 22.757268587330667 26.537500063555118 27.272149807046652 23.43308154206756 60-61 22.90088813005253 26.755835588317943 26.95707410959039 23.386202172039138 62-63 23.00698898223696 26.416206613071157 26.83529336716795 23.741511037523928 64-65 22.525280435768224 26.381990828215603 27.04354482525234 24.04918391076383 66-67 22.908294772738458 26.26789662863642 26.921583958828688 23.902224639796433 68-69 22.59916748850355 26.33441950477199 27.041459083369645 24.02495392335482 70-71 22.61112608146698 26.00558930947115 27.133084204014878 24.250200405046986 72-73 22.825495908071584 26.28421417070411 27.027573765315115 23.86271615590919 74-75 22.879203136030256 26.500833219044374 26.862851826665217 23.757111818260153 76-77 22.70731339755394 26.42416009671935 26.63844194205286 24.230084563673852 78-79 22.60109019939084 26.413751073624724 26.747725474750585 24.23743325223385 80-81 22.45765130470493 26.723433032856818 26.83970628530414 23.97920937713411 82-83 22.85440350302631 26.212979872526383 27.32858924512297 23.60402737932434 84-85 22.992924782513498 26.38572781461398 26.62576458378103 23.995582819091496 86-87 22.314930303530105 26.769772008893305 26.914409902679225 24.000887784897365 88-89 22.651355663438842 26.101984958524227 26.900066866670137 24.34659251136679 90-91 22.600109493593486 26.08703845860787 26.95099524470245 24.36185680309619 92-93 22.722598410091248 26.19929513638684 26.824059995431064 24.25404645809085 94-95 22.18990681407699 26.145442927511596 27.11973858617683 24.544911672234583 96-97 22.334065934959863 26.074774445537436 26.945178661721826 24.645980957780875 98-99 22.042956084021665 26.381278427378575 26.831771200242088 24.743994288357676 100 22.097389295927563 26.185648048788064 26.554226648587974 25.162736006696402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 524.0 1 376.5 2 319.0 3 421.0 4 364.0 5 366.5 6 618.0 7 1217.0 8 2027.0 9 2615.5 10 2584.0 11 2397.5 12 2650.5 13 3070.0 14 3576.0 15 4154.5 16 4740.0 17 5447.0 18 6309.0 19 7127.5 20 7999.0 21 9031.5 22 10026.5 23 11390.5 24 13483.0 25 15947.5 26 19254.5 27 23774.0 28 29891.5 29 39355.5 30 52436.5 31 66709.0 32 84772.0 33 104180.5 34 122749.0 35 141719.5 36 162815.5 37 192184.0 38 208758.5 39 210599.5 40 211262.0 41 210005.0 42 215569.0 43 229139.5 44 245709.0 45 273316.0 46 299392.5 47 317520.5 48 333056.0 49 334635.0 50 327102.0 51 324035.0 52 318929.0 53 308143.0 54 297296.0 55 271392.0 56 242079.5 57 208646.5 58 174814.0 59 155177.0 60 128252.5 61 98259.0 62 76168.5 63 59082.0 64 42823.5 65 27715.5 66 18866.0 67 13664.0 68 10106.0 69 7411.0 70 5469.5 71 4049.5 72 2878.5 73 2048.5 74 1570.5 75 1106.0 76 687.5 77 368.0 78 202.0 79 111.5 80 60.0 81 32.0 82 18.0 83 8.0 84 3.5 85 2.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005138352514030482 2 0.03229434218580635 3 0.0013828811515332695 4 0.0013150928597914424 5 0.006941521074363078 6 2.7115316696730774E-5 7 8.541324759470194E-4 8 0.006629694932350674 9 0.0010710550095208654 10-11 3.32162629534952E-4 12-13 1.152400959611058E-4 14-15 9.490360843855771E-4 16-17 7.456712091600963E-5 18-19 9.151419385146635E-4 20-21 1.9658604605129811E-4 22-23 0.0017150437810682215 24-25 0.0012744198847463464 26-27 0.002081100556474087 28-29 2.304801919222116E-4 30-31 0.0018506203645518755 32-33 0.0015998036851071157 34-35 7.931230133793752E-4 36-37 0.0041757587712965396 38-39 9.151419385146635E-4 40-41 0.004033403358638703 42-43 0.0040672975045096165 44-45 0.0039249420918517795 46-47 0.002413263186009039 48-49 0.009381899577068848 50-51 0.006650031419873222 52-53 0.004826526372018078 54-55 0.004528257888354039 56-57 0.0034029722454397126 58-59 0.005660322360442549 60-61 0.007829547696181011 62-63 0.0030436942992080294 64-65 0.0032673956619560583 66-67 0.00385037497093577 68-69 0.0020472064106031736 70-71 0.0029487906907694713 72-73 0.006378878252905915 74-75 0.008046470229754858 76-77 0.004589267350921683 78-79 0.0025759550861894237 80-81 0.0023861478693123083 82-83 0.004602825009270049 84-85 0.002169225335738462 86-87 0.003314847466175337 88-89 4.4740272549605777E-4 90-91 0.0017760532436358657 92-93 1.3557658348365388E-4 94-95 0.002257350115002837 96-97 0.0018506203645518755 98-99 0.001260862226397981 100 2.7115316696730774E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7375905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.14499497275308 #Duplication Level Percentage of deduplicated Percentage of total 1 71.78498594492918 41.02152660942129 2 16.301442264152964 18.630916724672915 3 5.51987233097982 9.46299229812242 4 2.3572015473053116 5.388090822821113 5 1.187113511656636 3.3918797827852862 6 0.7483360666751199 2.5658196460847726 7 0.4609423366052524 1.8438383255625344 8 0.3105852011300088 1.4198711805748685 9 0.22650072280652997 1.1649044399493684 >10 1.0346392629773355 10.072825008613354 >50 0.04800342285022081 1.8741550968414782 >100 0.019177484364018445 1.8982634123819768 >500 8.209906449301289E-4 0.3096414366871564 >1k 2.841846920991003E-4 0.24117807621727452 >5k 3.157607688638097E-5 0.14642825736269163 >10k+ 6.315215377276193E-5 0.5676688819014514 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16724 0.22673827821806272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14736 0.19978565342151236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8115 0.11002039749698511 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.3557658348365387E-5 10-11 0.0 1.3557658348365387E-5 0.0 0.0 1.3557658348365387E-5 12-13 0.0 1.3557658348365387E-5 0.0 0.0 1.3557658348365387E-5 14-15 0.0 3.389414587091347E-5 0.0 1.3557658348365387E-5 7.456712091600963E-5 16-17 0.0 5.423063339346155E-5 0.0 1.3557658348365387E-5 1.084612667869231E-4 18-19 0.0 5.423063339346155E-5 0.0 2.7115316696730774E-5 1.1524009596110579E-4 20-21 0.0 5.423063339346155E-5 0.0 4.7451804219278856E-5 1.287977543094712E-4 22-23 0.0 5.423063339346155E-5 0.0 2.1014370439966353E-4 2.1014370439966353E-4 24-25 0.0 5.423063339346155E-5 0.0 6.846617465924521E-4 2.8471082531567315E-4 26-27 0.0 5.423063339346155E-5 0.0 0.001416775297404183 2.9826848366403853E-4 28-29 0.0 5.423063339346155E-5 0.0 0.0038232596542390395 2.9826848366403853E-4 30-31 0.0 6.778829174182694E-5 0.0 0.013510206544146109 3.186049711865866E-4 32-33 0.0 6.778829174182694E-5 0.0 0.03983917905667169 3.389414587091347E-4 34-35 0.0 6.778829174182694E-5 0.0 0.08339315650079548 3.524991170575001E-4 36-37 0.0 7.456712091600963E-5 0.0 0.1545911993172363 3.524991170575001E-4 38-39 0.0 8.134595009019233E-5 0.0 0.26144588358987814 3.524991170575001E-4 40-41 0.0 8.134595009019233E-5 0.0 0.3760487696086108 3.524991170575001E-4 42-43 0.0 8.134595009019233E-5 0.0 0.49272597735464324 3.6605677540586546E-4 44-45 0.0 8.134595009019233E-5 0.0 0.6411213268066767 3.8639326292841354E-4 46-47 0.0 8.134595009019233E-5 0.0 0.8201637629551899 4.338450671476924E-4 48-49 0.0 8.134595009019233E-5 0.0 1.0152042359547744 4.4740272549605777E-4 50-51 0.0 8.134595009019233E-5 0.0 1.2173624795872506 4.4740272549605777E-4 52-53 0.0 8.134595009019233E-5 0.0 1.4104574286138445 4.88075700541154E-4 54-55 0.0 8.134595009019233E-5 0.0 1.6300440420531448 5.219698464120674E-4 56-57 0.0 8.134595009019233E-5 0.0 1.8886631538773886 5.355275047604328E-4 58-59 0.0 8.134595009019233E-5 0.0 2.1831626085205817 5.423063339346155E-4 60-61 0.0 9.490360843855771E-5 0.0 2.4894233317809813 5.423063339346155E-4 62-63 0.0 9.490360843855771E-5 0.0 2.803622064004349 5.694216506313463E-4 64-65 0.0 9.490360843855771E-5 0.0 3.128334760276874 5.694216506313463E-4 66-67 0.0 9.490360843855771E-5 0.0 3.4905072665659334 5.76200479805529E-4 68-69 0.0 9.490360843855771E-5 0.0 3.893427315021004 5.829793089797117E-4 70-71 0.0 9.490360843855771E-5 0.0 4.330234730517814 6.575464298957213E-4 72-73 0.0 9.490360843855771E-5 0.0 4.774512415764574 8.134595009019232E-4 74-75 0.0 9.490360843855771E-5 0.0 5.21458722692334 8.134595009019232E-4 76-77 0.0 9.490360843855771E-5 0.0 5.692975980574587 8.134595009019232E-4 78-79 1.3557658348365387E-5 9.490360843855771E-5 0.0 6.210085406468766 8.134595009019232E-4 80-81 1.3557658348365387E-5 9.490360843855771E-5 0.0 6.767135693857229 8.337959884244713E-4 82-83 1.3557658348365387E-5 9.490360843855771E-5 0.0 7.334171196619263 8.676901342953848E-4 84-85 1.3557658348365387E-5 9.490360843855771E-5 0.0 7.886435630610753 8.676901342953848E-4 86-87 1.3557658348365387E-5 9.490360843855771E-5 0.0 8.45777568989839 8.812477926437502E-4 88 1.3557658348365387E-5 9.490360843855771E-5 0.0 8.908899992611076 9.083631093404809E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11340 0.0 51.125694 1 GTATCAA 20280 0.0 41.595245 1 CAACGCA 24360 0.0 33.323086 5 ATCAACG 24740 0.0 32.79292 3 TATCAAC 25805 0.0 32.472126 2 AACGCAG 27020 0.0 30.804857 6 TCAACGC 27220 0.0 30.046524 4 GTACTAG 4115 0.0 28.566652 1 GTGGTAT 4090 0.0 27.591616 1 ACGCAGA 29650 0.0 27.32723 7 CGCAGAG 30630 0.0 26.330149 8 GTACATG 29465 0.0 25.596886 1 TACATGG 29990 0.0 24.742147 2 ACATGGG 29675 0.0 24.092257 3 GCAGAGT 33605 0.0 23.52128 9 TAGGCAT 5420 0.0 22.894691 5 GAGTACT 17115 0.0 22.22667 12-13 CATGGGG 23900 0.0 22.026894 4 CTAGGCA 6080 0.0 21.569185 4 TAGTACT 5870 0.0 21.54008 4 >>END_MODULE