FastQCFastQC Report
Thu 26 May 2016
ERR580209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5766396
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98070.17007156636484902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79600.1380411612383194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89050.050.4216841
GTATCAA162450.041.3001981
CAACGCA187400.034.1663745
ATCAACG191300.033.522183
TATCAAC202300.032.7698862
AACGCAG209050.031.1760986
TCAACGC210850.030.5024014
GTACTAG28400.029.1368261
CGTTAAC7750.028.5130751
ACGCAGA236700.026.9193427
GTGGTAT35200.026.4466381
CGCAGAG245150.025.7993418
GTACATG295850.025.4588531
TACATGG301750.024.5250072
ACATGGG304650.023.3336933
GCAGAGT268300.022.9296429
TAGGCAT44100.021.9593775
CATGGGG246550.021.7952964
GCATCGT32750.021.665128
CTAGGCA47250.020.8948384
Produced by FastQC (version 0.11.5)