FastQCFastQC Report
Thu 26 May 2016
ERR580209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5766396
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88850.1540823765832246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76480.13263050265711893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92250.048.4639631
GTATCAA162250.039.5013281
CAACGCA191350.032.2718245
ATCAACG196850.031.3232173
TATCAAC204900.030.9404492
AACGCAG212150.029.7718146
GTACTAG31600.029.1021121
TCAACGC214750.028.8212624
ACGCAGA241750.025.4461617
GTACATG297850.025.3020731
GTGGTAT36800.024.9898551
TACATGG302350.024.5896952
CGCAGAG251800.024.3187688
ACATGGG306700.023.5366923
GCAGAGT277050.021.627439
CATGGGG245350.021.4915244
CGTTAAC10150.020.4438931
GAGTACT94900.020.3784712-13
GCATCGT41650.020.310028
TGGTATC45950.019.4323232
Produced by FastQC (version 0.11.5)