FastQCFastQC Report
Thu 26 May 2016
ERR580208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613129
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53140.14707473771348878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.12006214004537342No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.046.1100161
GTATCAA98600.040.3022271
CAACGCA112400.033.885495
ATCAACG114700.033.1263123
TATCAAC121350.032.321652
GTACTAG17000.031.8125271
AACGCAG126450.030.9239546
TCAACGC127900.029.9280934
GTGGTAT21150.028.0162121
ACGCAGA142450.026.7232367
GTACATG183700.025.9328081
CGCAGAG148600.025.4904048
TACATGG186750.025.233322
ACATGGG189200.023.9596843
GCATCGT20600.023.954258
CATGGGG152400.022.833474
GCAGAGT163300.022.7954229
ATGGGGG111800.021.9545125
TAGGCAT27000.021.4208375
CGTTAAC7250.021.405491
Produced by FastQC (version 0.11.5)