FastQCFastQC Report
Thu 26 May 2016
ERR580208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613129
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47580.1316864136320624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41370.11449909482888654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.046.1687161
GTATCAA98900.039.6408581
CAACGCA113550.032.948245
ATCAACG117300.031.8174083
TATCAAC124850.031.0578692
AACGCAG125900.030.4221576
TCAACGC127650.029.4569264
GTACTAG19600.027.6808911
ACGCAGA144050.025.9039137
GTACATG183150.025.759141
GTGGTAT24650.025.0722141
CGCAGAG148750.024.9590638
TACATGG187350.024.811262
ACATGGG190100.023.7620323
TACCCCG4000.023.5004545
CATGGGG149350.022.5648374
TAGGCAT27200.022.4636675
GCAGAGT166250.021.9360399
GTATAAG20000.021.2300421
ATGGGGG107550.021.10785
Produced by FastQC (version 0.11.5)