FastQCFastQC Report
Thu 26 May 2016
ERR580207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593196
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144460.21910466486966262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122750.18617677981968078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68190.10342480338822022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95350.049.2164461
GTATCAA170550.039.5916061
ATCAACG190000.034.090073
CAACGCA190350.034.078585
TATCAAC209150.031.8043752
AACGCAG210550.031.4643026
TCAACGC216650.030.266414
ACGCAGA229000.028.3148527
GTACTAG43700.028.2992361
CGCAGAG233350.027.6861328
GCATCGT38950.026.1769878
GTACATG221400.025.7197631
TACATGG220500.025.0505682
GTGGTAT37800.024.7549291
GCAGAGT261350.024.3994929
ACATGGG219500.024.0265033
CTGTGCG46050.022.858199
CGTTAAC9700.022.2990591
TGGTATC40050.022.1843932
TAGGCAT57100.021.8154955
Produced by FastQC (version 0.11.5)