FastQCFastQC Report
Thu 26 May 2016
ERR580207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593196
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131070.1987958495394343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115390.17501375660605267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.10368264495701326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98700.049.9158481
GTATCAA171700.038.632341
ATCAACG195250.032.5455063
CAACGCA196750.032.296895
TATCAAC213550.030.7103982
AACGCAG219950.029.6372646
GTACTAG43500.029.5031071
TCAACGC220750.029.062574
GTGGTAT37000.026.8086571
ACGCAGA237950.026.605267
CGCAGAG242650.025.9737198
TAGGCAT52550.025.4006275
GCATCGT45000.024.9615178
GTACATG215550.024.4484831
TACATGG215550.023.816912
CGACGAG22900.023.70511424-25
ACATGGG216400.022.9139843
GCAGAGT272900.022.6282589
CTAGGCA64100.022.070424
CTGTGCG48700.021.519659
Produced by FastQC (version 0.11.5)