##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580207_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6593196
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4145364402939	34.0	31.0	34.0	31.0	34.0
2	32.55354262181801	34.0	31.0	34.0	31.0	34.0
3	32.60895459500976	34.0	31.0	34.0	31.0	34.0
4	36.158203244678305	37.0	35.0	37.0	35.0	37.0
5	36.09736067303323	37.0	35.0	37.0	35.0	37.0
6	36.13170896178424	37.0	36.0	37.0	35.0	37.0
7	36.07714877579856	37.0	35.0	37.0	35.0	37.0
8	36.06926352561034	37.0	35.0	37.0	35.0	37.0
9	37.776583314071054	39.0	38.0	39.0	35.0	39.0
10-11	37.72042367616555	39.0	37.5	39.0	35.0	39.0
12-13	37.68642938872134	39.0	37.0	39.0	35.0	39.0
14-15	39.04166932091811	40.0	38.0	41.0	36.0	41.0
16-17	39.03012810479167	40.0	38.0	41.0	36.0	41.0
18-19	39.0221510175035	40.0	38.5	41.0	35.5	41.0
20-21	38.99486819442346	40.0	38.0	41.0	35.0	41.0
22-23	38.9135279764169	40.0	38.0	41.0	35.0	41.0
24-25	38.8441944544042	40.0	38.0	41.0	35.0	41.0
26-27	38.71780089656063	40.0	38.0	41.0	35.0	41.0
28-29	38.61168354467242	40.0	38.0	41.0	34.0	41.0
30-31	38.461426522129784	40.0	38.0	41.0	34.0	41.0
32-33	38.35906470852679	40.0	38.0	41.0	34.0	41.0
34-35	38.266747113236136	40.0	38.0	41.0	34.0	41.0
36-37	38.13976931673197	40.0	38.0	41.0	33.5	41.0
38-39	38.02085847288629	40.0	37.0	41.0	33.0	41.0
40-41	37.90985881505722	40.0	37.0	41.0	33.0	41.0
42-43	37.920472408222054	40.0	37.0	41.0	33.0	41.0
44-45	37.89793864766041	40.0	37.0	41.0	33.0	41.0
46-47	37.73826373431034	40.0	37.0	41.0	33.0	41.0
48-49	37.562236584503175	40.0	37.0	41.0	33.0	41.0
50-51	37.39039898101012	40.0	36.5	41.0	32.0	41.0
52-53	37.23423480812644	40.0	36.0	41.0	32.0	41.0
54-55	37.0328932281097	39.0	36.0	41.0	31.0	41.0
56-57	36.81378757737522	39.0	35.0	41.0	31.0	41.0
58-59	36.5905084575068	39.0	35.0	40.5	31.0	41.0
60-61	36.352978206623916	38.0	35.0	40.0	30.5	41.0
62-63	36.070236953368294	38.0	35.0	40.0	30.0	41.0
64-65	35.786402224353715	37.5	35.0	40.0	30.0	41.0
66-67	35.47946231235959	37.0	34.0	40.0	29.0	41.0
68-69	35.16526810366323	36.5	34.0	39.0	29.0	41.0
70-71	34.84305653889252	36.0	34.0	39.0	29.0	41.0
72-73	34.51987329058623	35.5	34.0	39.0	29.0	40.0
74-75	34.15829796353695	35.0	34.0	37.5	28.0	40.0
76-77	32.602487549285655	34.0	31.5	36.0	26.0	39.0
78-79	33.50834034055714	35.0	33.0	37.0	28.0	39.0
80-81	33.47866148678122	35.0	33.0	36.0	28.0	38.5
82-83	33.22948733815892	35.0	33.0	36.0	28.0	37.0
84-85	32.993299455984626	35.0	33.0	35.5	27.0	37.0
86-87	32.77225089319353	35.0	33.0	35.0	27.0	36.5
88-89	32.57122721666397	35.0	33.0	35.0	27.0	36.0
90-91	32.391610002190134	35.0	33.0	35.0	26.0	36.0
92-93	32.22409223083919	35.0	32.5	35.0	26.0	36.0
94-95	32.08236255982683	35.0	32.0	35.0	25.0	35.0
96-97	31.950688709997397	34.0	32.0	35.0	25.0	35.0
98-99	31.84172296713157	34.0	32.0	35.0	25.0	35.0
100	29.90557295733359	33.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.020967509604538748
1101	2	-0.06729164494444717
1101	3	-0.044869031588802955
1101	4	-0.003012981410051907
1101	5	-0.03952071418082426
1101	6	-0.05401630058788953
1101	7	-0.043497877560987774
1101	8	-0.02249512036389234
1101	9	-0.04611583440763667
1101	10-11	-0.08972902073355016
1101	12-13	-0.03426969263477986
1101	14-15	-0.04798941447310767
1101	16-17	-0.00523266655946486
1101	18-19	-0.07061159901095948
1101	20-21	-0.0789076134036435
1101	22-23	-0.09422076153465753
1101	24-25	-0.09539435761457327
1101	26-27	-0.116121903186027
1101	28-29	-0.09745227655924538
1101	30-31	-0.06926138074749133
1101	32-33	-0.11790155233433808
1101	34-35	-0.16143564391133935
1101	36-37	-0.16624783646479813
1101	38-39	-0.20223364813944045
1101	40-41	-0.16057876537067983
1101	42-43	-0.1627337866246492
1101	44-45	-0.10199084375334877
1101	46-47	-0.17302625264471772
1101	48-49	-0.10190245896196615
1101	50-51	-0.11962451799099227
1101	52-53	-0.12295890895945405
1101	54-55	-0.1586449158566765
1101	56-57	-0.13283677097128788
1101	58-59	-0.17480728032499826
1101	60-61	-0.19861845458593308
1101	62-63	-0.21832678758722324
1101	64-65	-0.19533784539665078
1101	66-67	-0.1252224721556061
1101	68-69	-0.18112214212110445
1101	70-71	-0.25929254943952884
1101	72-73	-0.19778200437356475
1101	74-75	-0.27717080325421506
1101	76-77	-0.1942998470877626
1101	78-79	-0.18032475555473582
1101	80-81	-0.13938331035317475
1101	82-83	-0.16600045459529866
1101	84-85	-0.10219924851227802
1101	86-87	-0.11895587898138871
1101	88-89	-0.08986100435159727
1101	90-91	-0.15204160422970858
1101	92-93	-0.21881309563136142
1101	94-95	-0.19122731703583185
1101	96-97	-0.23074489473388837
1101	98-99	-0.18582458917490996
1101	100	-0.44335171362559933
1102	1	-0.08881948307067233
1102	2	-0.07535395773787457
1102	3	-0.05792814014903058
1102	4	-0.025556193357523682
1102	5	-0.05283982768300177
1102	6	-0.045372580181847866
1102	7	-0.013390992778788302
1102	8	-0.05139642495890229
1102	9	-0.07738684021543918
1102	10-11	-0.05068620504626864
1102	12-13	-0.02992434058096194
1102	14-15	-0.03602579708466891
1102	16-17	-0.07815846545817351
1102	18-19	-0.04314722727170306
1102	20-21	-0.06385643105164007
1102	22-23	-0.031282690623669396
1102	24-25	-0.05956909680606515
1102	26-27	-0.14311523255508263
1102	28-29	-0.14160628365878125
1102	30-31	-0.18226205474137203
1102	32-33	-0.10456119446467937
1102	34-35	-0.10286457120638914
1102	36-37	-0.16836777824618565
1102	38-39	-0.2719729300295981
1102	40-41	-0.22758287261505927
1102	42-43	-0.15273831172514463
1102	44-45	-0.15020326583222499
1102	46-47	-0.14527432859810574
1102	48-49	-0.17008970922764632
1102	50-51	-0.212753635028875
1102	52-53	-0.17705524237248227
1102	54-55	-0.18964354928636595
1102	56-57	-0.12305623834536306
1102	58-59	-0.17291954500470297
1102	60-61	-0.2164841640674524
1102	62-63	-0.15005936871257575
1102	64-65	-0.11942197710971669
1102	66-67	-0.1604020649066058
1102	68-69	-0.21676619801739605
1102	70-71	-0.20340714396842685
1102	72-73	-0.22646957204918294
1102	74-75	-0.25958281490999013
1102	76-77	-0.2365359096777908
1102	78-79	-0.17083996159999515
1102	80-81	-0.16286549320941646
1102	82-83	-0.15611285889519877
1102	84-85	-0.18170935741602534
1102	86-87	-0.15523518164381045
1102	88-89	-0.1871336393964711
1102	90-91	-0.23622855014345134
1102	92-93	-0.26935752649325195
1102	94-95	-0.23487606728443922
1102	96-97	-0.3302414710257757
1102	98-99	-0.32501428229173257
1102	100	-0.1451854842346414
1103	1	0.011187557114098468
1103	2	0.0046196436564898136
1103	3	0.00361601572041792
1103	4	-0.002895687642414657
1103	5	-0.018802830296507977
1103	6	0.004158054112771481
1103	7	0.021596564609339453
1103	8	-0.01533437379676883
1103	9	-0.04117661857927146
1103	10-11	0.035385959475121354
1103	12-13	0.03184878704605154
1103	14-15	-0.009867422099773648
1103	16-17	0.03380151682521415
1103	18-19	0.030599506267961374
1103	20-21	0.029867336310111625
1103	22-23	0.016222423332656888
1103	24-25	0.018540832376359617
1103	26-27	0.016260406436224173
1103	28-29	-0.02560476384324062
1103	30-31	-0.05190373929477943
1103	32-33	-0.021708128032031482
1103	34-35	0.019985277908482146
1103	36-37	-0.027660096515681687
1103	38-39	-0.014581736674571744
1103	40-41	0.034581391667344974
1103	42-43	0.06107988398722597
1103	44-45	0.07656372539096878
1103	46-47	0.02637457230431295
1103	48-49	0.043676378803937155
1103	50-51	-0.013418022692540887
1103	52-53	-0.042980387110652885
1103	54-55	-0.0036891623660082473
1103	56-57	0.09061998726498643
1103	58-59	0.06015830762745367
1103	60-61	-0.036702671070422355
1103	62-63	0.02713621309631975
1103	64-65	0.11219848115154463
1103	66-67	0.08328283164773609
1103	68-69	0.006515398347815449
1103	70-71	0.034255718010818725
1103	72-73	0.006076657003660557
1103	74-75	0.013699219778551708
1103	76-77	0.02700041712311929
1103	78-79	0.05940254071987283
1103	80-81	0.020648746515824712
1103	82-83	0.037032608977874304
1103	84-85	0.04773134471852103
1103	86-87	0.06333450033456955
1103	88-89	0.03974172941823184
1103	90-91	0.012022324913203875
1103	92-93	-0.016143603566504794
1103	94-95	0.021024618029791498
1103	96-97	-0.059175608906286925
1103	98-99	-0.06928755117672125
1103	100	-0.08281028206875263
1104	1	3.971508039697369E-4
1104	2	-0.033739442998417246
1104	3	-0.006145083439186294
1104	4	0.00200299307699936
1104	5	0.009144932147790996
1104	6	0.004979088627088402
1104	7	0.008047799743778228
1104	8	0.04099667519349737
1104	9	0.01560424750194045
1104	10-11	0.0043750234074622085
1104	12-13	-0.0176127381223381
1104	14-15	-0.10162677676117937
1104	16-17	-0.10529858479628729
1104	18-19	-0.054402455073955025
1104	20-21	-0.07358688596489316
1104	22-23	-0.08634139515881145
1104	24-25	-0.050507951291429265
1104	26-27	-0.06282690637418398
1104	28-29	-0.07740554745225836
1104	30-31	-0.10804560043930422
1104	32-33	-0.08118084435200501
1104	34-35	-0.08232518734055816
1104	36-37	-0.03693325609727793
1104	38-39	-0.05769319517099092
1104	40-41	-0.037234462921674094
1104	42-43	-0.08197105182328102
1104	44-45	-0.12382598579480941
1104	46-47	-0.08796015862592554
1104	48-49	-0.03150851231492169
1104	50-51	-0.021101917246255653
1104	52-53	-0.06471166464275058
1104	54-55	-0.08967824652518175
1104	56-57	-0.10923554993678408
1104	58-59	-0.13802324267587096
1104	60-61	-0.25244322934422314
1104	62-63	-0.18597093843030166
1104	64-65	-0.1301856808223505
1104	66-67	-0.11780695344951653
1104	68-69	-0.1116911314855713
1104	70-71	-0.06686905312179903
1104	72-73	-0.1518877533588423
1104	74-75	-0.12372784859536523
1104	76-77	-0.06775508481240422
1104	78-79	-0.09293266882535534
1104	80-81	-0.041434830361495756
1104	82-83	-0.012324709231606334
1104	84-85	-0.05489576793304707
1104	86-87	0.0066163515135642115
1104	88-89	-4.559372077039825E-4
1104	90-91	-0.0927584021469059
1104	92-93	-0.0535807335455587
1104	94-95	0.014422905268155262
1104	96-97	0.009356425509068345
1104	98-99	-0.05094086292473676
1104	100	-0.08762678398654344
1105	1	0.035138514071675786
1105	2	0.011201439593200746
1105	3	-0.010397684098798265
1105	4	0.017046985171916162
1105	5	0.024632989586756082
1105	6	0.011524375897238315
1105	7	-0.01452258850654431
1105	8	0.05539767559834985
1105	9	0.05740283101734889
1105	10-11	0.023461447655371614
1105	12-13	0.035234919589626656
1105	14-15	0.04181756000732406
1105	16-17	0.019677393148427313
1105	18-19	-0.0072972261930033255
1105	20-21	-0.028268043315847535
1105	22-23	0.0010091215405623188
1105	24-25	-0.047297738507275255
1105	26-27	-0.01997086864512454
1105	28-29	0.02163657438945421
1105	30-31	-0.011985438661845649
1105	32-33	-0.07916461583242551
1105	34-35	-0.04865229007496907
1105	36-37	-0.0445576871183988
1105	38-39	-0.05742762216382857
1105	40-41	-0.013267201659900252
1105	42-43	-0.04147803332021027
1105	44-45	-0.004050129136409453
1105	46-47	-0.033160212182842486
1105	48-49	-0.11707774552538552
1105	50-51	-0.08876996785053848
1105	52-53	-0.15379415367370086
1105	54-55	-0.08568729102768913
1105	56-57	-0.15570960521863242
1105	58-59	-0.13088278897268424
1105	60-61	-0.044073583601750954
1105	62-63	-0.0782488664798322
1105	64-65	-0.14376131542964288
1105	66-67	-0.23956707449256953
1105	68-69	-0.222538604715524
1105	70-71	-0.0999063997175682
1105	72-73	-0.12813667331664647
1105	74-75	-0.09592861165241118
1105	76-77	-0.14842833404055256
1105	78-79	-0.019075129208147246
1105	80-81	-0.0013914291711287774
1105	82-83	0.10744874003788851
1105	84-85	0.046075478720737806
1105	86-87	0.057290782090063885
1105	88-89	0.12460235270393838
1105	90-91	0.07187458531029733
1105	92-93	0.05778482359817616
1105	94-95	0.06953085926726033
1105	96-97	-0.010989431729406363
1105	98-99	0.016545642911765412
1105	100	-0.028068857993780227
1106	1	-0.052530139857360325
1106	2	-0.04064121255200348
1106	3	-0.03982245036173282
1106	4	-0.0026624956960930035
1106	5	-0.025415764667286567
1106	6	-0.05809788438715913
1106	7	-0.03884416702172899
1106	8	-0.03255613031848981
1106	9	0.018836720706907784
1106	10-11	-0.026650017583072838
1106	12-13	-0.019358349045191403
1106	14-15	-0.02691197247903432
1106	16-17	-0.03452427424076632
1106	18-19	-0.020769826859066143
1106	20-21	-0.06089737905181636
1106	22-23	-0.03947139144202083
1106	24-25	-0.04587880425362556
1106	26-27	-0.046637159899091785
1106	28-29	0.02085929946709797
1106	30-31	0.013919294708465202
1106	32-33	-0.010251979845236292
1106	34-35	0.00414250027454699
1106	36-37	-0.034470042623802044
1106	38-39	0.01745194265922123
1106	40-41	-0.0684352873057179
1106	42-43	-0.006940469463003751
1106	44-45	0.08936180016781492
1106	46-47	0.037250437215554655
1106	48-49	-0.02434544342737155
1106	50-51	-0.028308720364925932
1106	52-53	-0.020097500783244016
1106	54-55	-0.016241847428638323
1106	56-57	0.03520526384541256
1106	58-59	-0.009699548329457741
1106	60-61	0.004357505670760986
1106	62-63	-0.060253036793973536
1106	64-65	-0.10649152401810369
1106	66-67	-0.03968006652905842
1106	68-69	-0.046285307842126144
1106	70-71	0.012022985333985048
1106	72-73	0.01898073646508891
1106	74-75	0.006777049615948272
1106	76-77	-0.09163142431572169
1106	78-79	0.02716266976698023
1106	80-81	0.07995376612565508
1106	82-83	0.01800839414807598
1106	84-85	0.07827686194588068
1106	86-87	0.15166086697913528
1106	88-89	0.08615717377981724
1106	90-91	0.041848552505513226
1106	92-93	0.05885910564609986
1106	94-95	-0.00410115438267411
1106	96-97	0.007768472257090764
1106	98-99	0.01680508631484301
1106	100	0.1683005868974803
1107	1	0.016581677949496054
1107	2	0.01406693421630223
1107	3	-0.0224831136071586
1107	4	9.169859180602202E-4
1107	5	-0.013069586745331208
1107	6	0.005229233276793366
1107	7	-0.007141038882920725
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2316	32-33	-0.22330309864368303
2316	34-35	-0.30224098914234077
2316	36-37	-0.28748760887828695
2316	38-39	-0.2693424202477388
2316	40-41	-0.2634702839220324
2316	42-43	-0.3680143458538154
2316	44-45	-0.32197669226153636
2316	46-47	-0.25899774619934135
2316	48-49	-0.2771183645847728
2316	50-51	-0.2105578443000482
2316	52-53	-0.286987506235981
2316	54-55	-0.3166234324751329
2316	56-57	-0.43632477762739086
2316	58-59	-0.3881752627110231
2316	60-61	-0.343762803435645
2316	62-63	-0.30111991784634284
2316	64-65	-0.2960058176095828
2316	66-67	-0.37845933070609306
2316	68-69	-0.2682090420905894
2316	70-71	-0.2803496604279232
2316	72-73	-0.25960812813644196
2316	74-75	-0.2062344976837167
2316	76-77	-0.244447356861194
2316	78-79	-0.2722628559485827
2316	80-81	-0.26007882256814696
2316	82-83	-0.3295188533512672
2316	84-85	-0.223686263292322
2316	86-87	-0.2787643706031204
2316	88-89	-0.27820064742154926
2316	90-91	-0.22441122520952206
2316	92-93	-0.29812802899763824
2316	94-95	-0.3150418798110515
2316	96-97	-0.264094365733321
2316	98-99	-0.18893128669994042
2316	100	-0.5309327350036135
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	46.0
14	257.0
15	917.0
16	2395.0
17	5504.0
18	9798.0
19	15116.0
20	22459.0
21	31637.0
22	39735.0
23	39202.0
24	37730.0
25	39457.0
26	47032.0
27	58124.0
28	72717.0
29	89748.0
30	111239.0
31	139793.0
32	178274.0
33	236997.0
34	343265.0
35	509851.0
36	879400.0
37	1569019.0
38	1740786.0
39	372573.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10439795542419	21.060119744711663	13.956873641617392	23.878608658246748
2	15.136350836990717	21.27913264166699	40.27288069969317	23.311635821649123
3	18.004328458698314	26.72032268843121	30.329879272434297	24.945469580436182
4	12.256122115452431	17.837392245383622	39.33679199709393	30.56969364207001
5	12.762647566955923	38.41902569026373	35.613437144022924	13.204889598757427
6	29.937614455148747	38.58839888527472	18.115271596740516	13.35871506283601
7	26.078023639758353	32.77305316291352	23.356797150973815	17.792126046354312
8	25.076940391938678	36.01767452386449	20.525057312818323	18.380327771378507
9	25.72493751061713	15.57340075715291	21.205317057781446	37.496344674448515
10-11	24.54977919233681	26.43103333345214	28.780786635405075	20.23840083880597
12-13	25.089036992962356	24.209219623925396	28.71620573754436	21.98553764556789
14-15	22.426316294783053	25.76291716232021	26.3918656750714	25.418900867825332
16-17	21.662215103766158	28.355219552295623	27.343319394708782	22.639245949229434
18-19	21.170031435790207	28.028216374579447	29.34262402546168	21.459128164168657
20-21	22.463211051718954	27.21913127689094	28.987101449110504	21.330556222279604
22-23	22.443443649546854	27.0745101786453	28.585348984562252	21.89669718724559
24-25	22.017527361855997	27.088593867546777	29.07671936806853	21.817159402528695
26-27	22.056000488391785	27.359609165233028	28.536671283890236	22.04771906248495
28-29	21.578943217402465	27.570056461356597	28.881577969484233	21.96942235175671
30-31	22.289166997000414	27.222248723073943	28.802322192535367	21.686262087390272
32-33	22.191507351232055	27.4142724070402	28.42176837421244	21.972451867515304
34-35	22.322846033056436	27.495594290376268	28.718545569044473	21.463014107522824
36-37	22.192785009419165	27.180324710978716	28.49247831771562	22.134411961886503
38-39	22.171293027958374	27.25501602764267	28.819232655515226	21.754458288883725
40-41	22.199292032498917	26.80929409833947	29.02847250050736	21.962941368654253
42-43	21.9945875493918	26.96493692426164	28.65057158809255	22.389903938254015
44-45	22.13600249781036	27.402805820043746	28.502969335009563	21.95822234713633
46-47	22.073908602371116	27.207717340468523	28.08855417945128	22.629819877709075
48-49	22.40351319872685	27.176225492594035	28.23672878684354	22.183532521835573
50-51	22.31970109669422	27.183106335844542	27.99933411879002	22.497858448671217
52-53	22.003800301764134	27.457085446876732	27.964426869976368	22.57468738138277
54-55	21.954025560062508	27.405183073767585	27.961933306309323	22.678858059860584
56-57	22.40614391673099	27.200385318170365	27.704898215423558	22.688572549675087
58-59	21.89299583214897	27.409164418522746	28.10588932306321	22.591950426265075
60-61	22.116126985032263	27.625671309266565	27.73238760243505	22.52581410326612
62-63	22.147178877247846	27.27812486704658	27.649859786990895	22.92483646871468
64-65	21.6910724999198	27.21608173889448	27.870253474389138	23.22259228679658
66-67	21.950224364168804	27.144056364932652	27.749899153863183	23.155820117035365
68-69	21.790139935022584	27.134790218805836	27.854410546847745	23.220659299323835
70-71	21.717371269996892	26.781708969965827	27.890335299856332	23.61058446018094
72-73	21.804582955869645	26.905619352027728	27.95314887193183	23.3366488201708
74-75	21.93129914280351	27.078558332401116	27.7056826002954	23.28445992449997
76-77	21.630436579664604	27.0202500899072	27.593517520111384	23.755795810316812
78-79	21.559099277078282	26.978657409426233	27.657934601464046	23.804308712031442
80-81	21.392623869138294	27.249318276942653	27.708749981893778	23.64930787202528
82-83	21.796680270722067	26.739112925794117	28.16582477448802	23.298382028995796
84-85	21.807391956812758	26.83198752384705	27.60988906816837	23.750731451171823
86-87	21.06852090335264	27.187702180207456	27.9089776411205	23.834799275319398
88-89	21.47556654336974	26.534451410836606	27.826001558432694	24.16398048736096
90-91	21.39186463254294	26.55784442968317	27.784896777703093	24.265394160070798
92-93	21.45329824459757	26.538530899083888	27.789261008595386	24.218909847723157
94-95	20.864455153712786	26.487086463716373	28.127838692998058	24.520619689572783
96-97	20.91642367180716	26.3952328769201	27.993022982447492	24.695320468825244
98-99	20.64457465618451	26.53856318890599	27.90699984794727	24.909862306962232
100	20.63493676507525	26.403735853589584	27.63403522906659	25.327292152268583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	457.0
1	313.0
2	223.5
3	286.5
4	267.5
5	282.0
6	420.5
7	775.5
8	1294.5
9	1670.0
10	1711.5
11	1691.5
12	1883.0
13	2208.0
14	2589.5
15	3066.5
16	3615.0
17	4198.0
18	4834.0
19	5555.0
20	6421.0
21	7532.5
22	9022.0
23	10880.5
24	13457.0
25	16984.5
26	21728.0
27	27811.5
28	35386.5
29	46555.0
30	61393.0
31	77053.5
32	96627.0
33	117701.5
34	138294.0
35	158862.5
36	180629.0
37	208164.5
38	224463.0
39	227989.5
40	227702.0
41	226026.0
42	229491.0
43	238080.5
44	248874.5
45	267996.5
46	285233.0
47	293580.5
48	298255.0
49	291792.0
50	278291.0
51	269758.0
52	258032.5
53	239050.0
54	222287.5
55	195507.0
56	168332.5
57	139716.0
58	111670.0
59	97503.0
60	78498.5
61	57197.0
62	42716.5
63	33108.0
64	23670.0
65	14040.0
66	9230.5
67	6685.0
68	4841.5
69	3499.5
70	2604.5
71	1848.5
72	1252.5
73	905.5
74	688.0
75	464.0
76	288.5
77	180.0
78	102.0
79	52.5
80	31.5
81	13.5
82	7.0
83	6.0
84	3.0
85	2.0
86	3.0
87	2.5
88	1.0
89	1.0
90	2.5
91	1.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005111329922544393
2	0.029788284771148924
3	0.0013195421461761488
4	0.0013802107505980407
5	0.006461206370931488
6	3.033430221094595E-5
7	0.0010010319729612163
8	0.006521874975353379
9	0.0011527034840159462
10-11	2.1992369102935815E-4
12-13	1.4408793550199327E-4
14-15	7.431904041681758E-4
16-17	9.100290663283785E-5
18-19	0.001122369181805
20-21	2.0475653992388514E-4
22-23	0.0015698001394164529
24-25	0.0011527034840159462
26-27	0.0019489789170532776
28-29	2.1234011547662166E-4
30-31	0.0017669731037876016
32-33	0.0017821402548930747
34-35	7.128561019572299E-4
36-37	0.0038827906830010816
38-39	8.417768863537501E-4
40-41	0.003609781963102568
42-43	0.0037766206252627704
44-45	0.003359523969862264
46-47	0.002237154788057264
48-49	0.008668026856777806
50-51	0.006499124248695171
52-53	0.004527394604983683
54-55	0.004451558849456318
56-57	0.003078931674411014
58-59	0.0053085028869155415
60-61	0.007310566832837974
62-63	0.002904509436698075
64-65	0.0030713480988582773
66-67	0.0035111954809169937
68-69	0.0020627325503443246
70-71	0.0026087499901413518
72-73	0.006044109715530981
74-75	0.007333317559496184
76-77	0.004352972367270744
78-79	0.0024039934502174667
80-81	0.0024191606013229394
82-83	0.00468664969159115
84-85	0.0020930668525552705
86-87	0.003374691120967737
88-89	5.080995620333447E-4
90-91	0.0018124745571040206
92-93	1.0617005773831083E-4
94-95	0.0023205741191373655
96-97	0.00183522528376223
98-99	0.0013422928728343583
100	6.06686044218919E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6593196.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.34683159744627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49335948596865	47.934116579716516
2	16.092726607907675	20.710319379655566
3	4.8920366489360365	9.443611752528676
4	1.9395029905559968	4.992034892642007
5	0.9312044189270021	2.9960026963746818
6	0.4944675329778176	1.9090451444957006
7	0.28968139178062213	1.3048055813675086
8	0.1872925555826843	0.9641346026827468
9	0.1277426601945152	0.7397851899011646
>10	0.5133182987930215	5.501240753634246
>50	0.025693218015672155	1.1323308363183162
>100	0.012086293943914155	1.3826243187401348
>500	7.160470987144896E-4	0.3045943968345972
>1k	8.592465885341513E-5	0.10514276114244926
>5k	2.864155293344296E-5	0.12598701401022377
>10k+	5.728310586688592E-5	0.45422409995552593
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13107	0.1987958495394343	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11539	0.17501375660605267	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6836	0.10368264495701326	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.5167151105472975E-5	0.0
10-11	0.0	0.0	0.0	4.5501453316418925E-5	0.0
12-13	0.0	0.0	0.0	4.5501453316418925E-5	0.0
14-15	0.0	0.0	0.0	4.5501453316418925E-5	7.5835755527364875E-6
16-17	0.0	0.0	0.0	4.5501453316418925E-5	2.2750726658209462E-5
18-19	0.0	0.0	0.0	5.308502886915541E-5	3.791787776368244E-5
20-21	0.0	0.0	0.0	9.100290663283786E-5	4.5501453316418925E-5
22-23	0.0	0.0	0.0	1.895893888184122E-4	4.5501453316418925E-5
24-25	0.0	0.0	0.0	4.7776525982239873E-4	4.5501453316418925E-5
26-27	0.0	0.0	0.0	0.0012816242684124664	4.5501453316418925E-5
28-29	0.0	0.0	0.0	0.0038827906830010816	4.5501453316418925E-5
30-31	0.0	0.0	0.0	0.015478077703135171	4.5501453316418925E-5
32-33	0.0	0.0	0.0	0.04517535956765126	4.5501453316418925E-5
34-35	0.0	0.0	0.0	0.09493119876915534	6.06686044218919E-5
36-37	0.0	0.0	0.0	0.17636363305443975	6.06686044218919E-5
38-39	0.0	1.5167151105472975E-5	0.0	0.29297627432886875	6.06686044218919E-5
40-41	0.0	1.5167151105472975E-5	0.0	0.4158453654343053	6.06686044218919E-5
42-43	0.0	1.5167151105472975E-5	0.0	0.5423545728050554	6.06686044218919E-5
44-45	0.0	1.5167151105472975E-5	0.0	0.7033462982140983	7.583575552736488E-5
46-47	0.0	1.5167151105472975E-5	0.0	0.8989418788702778	7.583575552736488E-5
48-49	0.0	1.5167151105472975E-5	0.0	1.110114123711778	7.583575552736488E-5
50-51	0.0	1.5167151105472975E-5	0.0	1.3262611941158733	7.583575552736488E-5
52-53	0.0	1.5167151105472975E-5	0.0	1.537623028346192	9.100290663283786E-5
54-55	0.0	1.5167151105472975E-5	0.0	1.780949633531295	1.0617005773831083E-4
56-57	0.0	1.5167151105472975E-5	0.0	2.0657508134143137	1.0617005773831083E-4
58-59	0.0	1.5167151105472975E-5	0.0	2.383532963376184	1.0617005773831083E-4
60-61	0.0	1.5167151105472975E-5	0.0	2.711484688154273	1.0617005773831083E-4
62-63	0.0	1.5167151105472975E-5	0.0	3.0448738366036743	1.0617005773831083E-4
64-65	0.0	1.5167151105472975E-5	0.0	3.3923532684300604	1.0617005773831083E-4
66-67	0.0	1.5167151105472975E-5	0.0	3.7842269515421654	1.0617005773831083E-4
68-69	0.0	1.5167151105472975E-5	0.0	4.222785125756916	1.0617005773831083E-4
70-71	0.0	1.5167151105472975E-5	0.0	4.694870287490316	1.0617005773831083E-4
72-73	0.0	1.5167151105472975E-5	0.0	5.167054035705901	1.3650435994925678E-4
74-75	0.0	1.5167151105472975E-5	0.0	5.6312598624400065	1.3650435994925678E-4
76-77	0.0	1.5167151105472975E-5	0.0	6.140663799468422	1.3650435994925678E-4
78-79	0.0	1.5167151105472975E-5	0.0	6.69738166437036	1.3650435994925678E-4
80-81	0.0	1.5167151105472975E-5	0.0	7.2901670146011135	1.3650435994925678E-4
82-83	0.0	1.5167151105472975E-5	0.0	7.893258444008035	1.3650435994925678E-4
84-85	0.0	1.5167151105472975E-5	0.0	8.487370919960517	1.3650435994925678E-4
86-87	0.0	1.5167151105472975E-5	0.0	9.09858435878442	1.3650435994925678E-4
88	0.0	1.5167151105472975E-5	0.0	9.586185516098718	1.3650435994925678E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9870	0.0	49.915848	1
GTATCAA	17170	0.0	38.63234	1
ATCAACG	19525	0.0	32.545506	3
CAACGCA	19675	0.0	32.29689	5
TATCAAC	21355	0.0	30.710398	2
AACGCAG	21995	0.0	29.637264	6
GTACTAG	4350	0.0	29.503107	1
TCAACGC	22075	0.0	29.06257	4
GTGGTAT	3700	0.0	26.808657	1
ACGCAGA	23795	0.0	26.60526	7
CGCAGAG	24265	0.0	25.973719	8
TAGGCAT	5255	0.0	25.400627	5
GCATCGT	4500	0.0	24.961517	8
GTACATG	21555	0.0	24.448483	1
TACATGG	21555	0.0	23.81691	2
CGACGAG	2290	0.0	23.705114	24-25
ACATGGG	21640	0.0	22.913984	3
GCAGAGT	27290	0.0	22.628258	9
CTAGGCA	6410	0.0	22.07042	4
CTGTGCG	4870	0.0	21.51965	9
>>END_MODULE