FastQCFastQC Report
Thu 26 May 2016
ERR580206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9314320
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294710.31640527703579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241220.25897757431567736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138070.14823411692963093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165900.050.5359231
GTATCAA303750.041.270441
CAACGCA346900.034.7504125
ATCAACG351800.034.2958873
TATCAAC378850.032.8536952
GTACTAG68850.031.6890541
AACGCAG390450.031.5680796
TCAACGC399700.030.4673864
GTGGTAT53500.030.1464081
GCATCGT67400.029.0724838
ACGCAGA421150.028.3288887
TAGGCAT84550.028.248565
CGCAGAG424050.028.024498
GTACATG416100.026.7595751
GGGCACC85150.026.26906294
CTGTGCG95050.026.05699
TACATGG424800.025.8795992
ACATGGG417450.025.6922453
CTAGGCA98750.025.61624
GCAGAGT462000.025.3190369
Produced by FastQC (version 0.11.5)