FastQCFastQC Report
Thu 26 May 2016
ERR580206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9314320
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265890.2854636731398535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227740.2445052349500554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130010.13958077454929613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173000.049.1638261
GTATCAA306100.040.007131
CAACGCA353400.033.3528985
ATCAACG358200.032.9070173
TATCAAC389250.031.3439222
AACGCAG394650.030.5925226
TCAACGC404600.029.470074
GTGGTAT61400.029.2720171
ACGCAGA426300.027.6158247
CGCAGAG430300.027.2169848
TAGGCAT87550.026.1423995
GGGCACC85450.025.73987494
CTGTGCG97400.025.0418859
TAGTACT100900.024.640594
GTACATG412150.024.6310541
GCAGAGT470750.024.498699
TACATGG417150.023.9974252
CTAGGCA100450.023.9555764
CTAACGC32600.023.6435593
ACATGGG412450.023.4167753
Produced by FastQC (version 0.11.5)