##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580206_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9314320
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50000569016311	34.0	31.0	34.0	31.0	34.0
2	32.70230795162717	34.0	31.0	34.0	31.0	34.0
3	32.782169176064386	34.0	31.0	34.0	31.0	34.0
4	36.26482856504823	37.0	37.0	37.0	35.0	37.0
5	36.19825172422678	37.0	37.0	37.0	35.0	37.0
6	36.228687762499035	37.0	37.0	37.0	35.0	37.0
7	36.19384002267476	37.0	37.0	37.0	35.0	37.0
8	36.185770727224316	37.0	37.0	37.0	35.0	37.0
9	37.97056199486382	39.0	38.0	39.0	35.0	39.0
10-11	37.917156324884694	39.0	38.0	39.0	35.0	39.0
12-13	37.871142981989024	39.0	38.0	39.0	35.0	39.0
14-15	39.24528892071562	41.0	39.0	41.0	36.0	41.0
16-17	39.23492391285676	41.0	39.0	41.0	36.0	41.0
18-19	39.24620101091652	41.0	39.0	41.0	36.0	41.0
20-21	39.23527917228526	41.0	39.0	41.0	36.0	41.0
22-23	39.15770437348083	40.5	39.0	41.0	35.5	41.0
24-25	39.07085756126051	40.5	39.0	41.0	35.0	41.0
26-27	38.92585148459577	40.0	38.0	41.0	35.0	41.0
28-29	38.81886052873425	40.0	38.0	41.0	35.0	41.0
30-31	38.69201160149103	40.0	38.0	41.0	35.0	41.0
32-33	38.53536398792397	40.0	38.0	41.0	34.5	41.0
34-35	38.49796120382379	40.0	38.0	41.0	34.5	41.0
36-37	38.53445136091524	40.0	38.0	41.0	34.5	41.0
38-39	38.429682091661014	40.0	38.0	41.0	34.0	41.0
40-41	38.293041091566536	40.0	38.0	41.0	34.0	41.0
42-43	38.25006500742942	40.0	38.0	41.0	34.0	41.0
44-45	38.124427064992396	40.0	38.0	41.0	33.5	41.0
46-47	37.88773796691546	40.0	37.0	41.0	33.0	41.0
48-49	37.63219440603286	40.0	37.0	41.0	33.0	41.0
50-51	37.44077275635795	40.0	36.5	41.0	32.5	41.0
52-53	37.251020579065354	40.0	36.0	41.0	32.0	41.0
54-55	37.04226717570365	39.0	35.5	41.0	32.0	41.0
56-57	36.81427758548128	39.0	35.0	41.0	31.0	41.0
58-59	36.56361124590953	39.0	35.0	41.0	31.0	41.0
60-61	36.30128109191009	38.0	35.0	40.0	31.0	41.0
62-63	36.011936566491165	37.5	35.0	40.0	30.5	41.0
64-65	35.723479652835636	37.0	35.0	40.0	30.0	41.0
66-67	35.41222697953259	36.5	35.0	39.5	30.0	41.0
68-69	35.081562636886	36.0	34.0	39.0	29.0	41.0
70-71	34.78668018706679	35.5	34.0	39.0	29.0	40.5
72-73	34.46264214671602	35.0	34.0	38.5	29.0	40.0
74-75	34.12504079739584	35.0	34.0	37.0	29.0	39.5
76-77	32.90622890345189	34.5	32.0	36.0	26.5	39.0
78-79	33.55562096857312	35.0	33.5	36.0	28.5	39.0
80-81	33.506998900617546	35.0	34.0	36.0	29.0	38.5
82-83	33.30021021395014	35.0	34.0	36.0	29.0	37.0
84-85	33.084920530967366	35.0	33.5	35.0	29.0	37.0
86-87	32.90953134528339	35.0	33.0	35.0	28.0	36.5
88-89	32.74824431627859	35.0	33.0	35.0	27.0	36.0
90-91	32.57852237200353	35.0	33.0	35.0	27.0	36.0
92-93	32.4495944416769	35.0	33.0	35.0	27.0	36.0
94-95	32.34707836965018	35.0	33.0	35.0	27.0	35.0
96-97	32.24442025826899	35.0	33.0	35.0	26.5	35.0
98-99	32.1701327096342	35.0	33.0	35.0	26.0	35.0
100	30.86174320830721	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.13282091853610467
1101	2	0.09533458721845278
1101	3	0.037559532632414516
1101	4	0.029620018717537278
1101	5	0.009693875746108915
1101	6	0.02535731980657374
1101	7	-0.017955704695062025
1101	8	0.017998375481127482
1101	9	-0.017612768868950468
1101	10-11	-0.01968841255474274
1101	12-13	-0.039518037652293
1101	14-15	0.0349703410968516
1101	16-17	-0.010682578193872416
1101	18-19	-0.05042325782516599
1101	20-21	-0.06923817384139141
1101	22-23	-0.1296618707797066
1101	24-25	-0.1163301052089949
1101	26-27	-0.09818311272420743
1101	28-29	-0.11931921754339925
1101	30-31	-0.0965689832639427
1101	32-33	-0.07717425528919364
1101	34-35	-0.12897219013299122
1101	36-37	-0.07133951689635154
1101	38-39	-0.007604195134767622
1101	40-41	0.022728422706776996
1101	42-43	-0.01393921614705107
1101	44-45	-0.031410540077885685
1101	46-47	-0.02951192908032141
1101	48-49	-0.007555736933802848
1101	50-51	0.010236564128959458
1101	52-53	-0.051249574441932566
1101	54-55	-0.031183569068595318
1101	56-57	-0.019036732094001252
1101	58-59	-0.048067989280703216
1101	60-61	-0.059656741972212046
1101	62-63	-0.020353305739682526
1101	64-65	-0.056127295292952795
1101	66-67	-0.09210080212071858
1101	68-69	-0.07237182925079821
1101	70-71	-0.06697992940459585
1101	72-73	-0.03989452112728742
1101	74-75	-0.053579635063456976
1101	76-77	-0.060003633149896984
1101	78-79	-0.08481183010507465
1101	80-81	-0.06681037582311689
1101	82-83	0.015026622655611277
1101	84-85	-0.021145363395056904
1101	86-87	-0.0141332943217094
1101	88-89	-0.03771425416829999
1101	90-91	0.0192642374539318
1101	92-93	-0.09588779381731172
1101	94-95	-0.11255081172871684
1101	96-97	-0.10903508135456974
1101	98-99	-0.09556141358734749
1101	100	-0.0886566189168505
1102	1	0.11491847372028019
1102	2	0.061957158211939145
1102	3	-0.012524165325004333
1102	4	0.020816205575464153
1102	5	0.04159604408796724
1102	6	0.06439071626721216
1102	7	-0.009632148261530915
1102	8	-0.019129178227238697
1102	9	-0.008336531531170976
1102	10-11	0.018021941944780906
1102	12-13	0.019112718179705723
1102	14-15	0.07152189593310254
1102	16-17	-0.0035490484156213142
1102	18-19	0.028957920069338172
1102	20-21	-0.013918544107639264
1102	22-23	-0.03966351560740122
1102	24-25	-0.03302514755815622
1102	26-27	-0.0794158623017367
1102	28-29	-0.08267535585623165
1102	30-31	-0.06900004959322814
1102	32-33	-0.11712257856805053
1102	34-35	-0.08164296021191575
1102	36-37	-0.07899452713495947
1102	38-39	-0.12196839846240692
1102	40-41	0.014850391970895771
1102	42-43	-0.009995442871087334
1102	44-45	-0.006796112699156254
1102	46-47	-0.013757241733621584
1102	48-49	-0.048996944031209466
1102	50-51	-0.11882160505826533
1102	52-53	-0.07450526696110416
1102	54-55	-0.09558049718454242
1102	56-57	-0.1499460882092265
1102	58-59	-0.11875006236272156
1102	60-61	-0.15044889483868218
1102	62-63	-0.1477074224397299
1102	64-65	-0.12658634787769074
1102	66-67	-0.1765556963282009
1102	68-69	-0.09609400287479986
1102	70-71	-0.10620347042044642
1102	72-73	-0.11847489328982164
1102	74-75	-0.14991758490734242
1102	76-77	-0.15581261355185205
1102	78-79	-0.07917829524254927
1102	80-81	-0.040026548505572634
1102	82-83	-0.006352118090681813
1102	84-85	-0.04694830589149035
1102	86-87	-0.051094956782186784
1102	88-89	-0.08711841293031597
1102	90-91	-0.04258657433792479
1102	92-93	-0.06165208629818153
1102	94-95	-0.12661051581363125
1102	96-97	-0.14752982079159693
1102	98-99	-0.13670948545628647
1102	100	-0.05508200296419119
1103	1	0.12313468873089306
1103	2	0.07366788906854538
1103	3	0.07014167583079
1103	4	0.03339615973180088
1103	5	0.015975124574040933
1103	6	0.0818648099434256
1103	7	0.037976121220687276
1103	8	0.03571077853670346
1103	9	0.053602254276704286
1103	10-11	0.0387495135811875
1103	12-13	-0.009804496819761255
1103	14-15	0.026420964287083848
1103	16-17	0.05442219803283166
1103	18-19	0.035214751426529745
1103	20-21	-0.010601287309462748
1103	22-23	-0.027776592699190417
1103	24-25	-0.029870280699313412
1103	26-27	-0.014105953596690313
1103	28-29	-0.0197202045293281
1103	30-31	-0.0676192532395774
1103	32-33	-0.0672048474177771
1103	34-35	0.01403307550754107
1103	36-37	0.014066748323401157
1103	38-39	0.028275441148927882
1103	40-41	0.02520837814840604
1103	42-43	-0.0049858956643547
1103	44-45	0.019970588648327237
1103	46-47	0.019818203365566944
1103	48-49	0.09243182034174424
1103	50-51	0.06284991953888408
1103	52-53	0.06389914828297805
1103	54-55	0.10294619444845665
1103	56-57	0.132259918883058
1103	58-59	0.13279525792269453
1103	60-61	0.1098250739920914
1103	62-63	0.007310949858243987
1103	64-65	0.030892965130945527
1103	66-67	0.03166262513944673
1103	68-69	0.059014272458682626
1103	70-71	0.08464914353441344
1103	72-73	0.06215765235248938
1103	74-75	-0.0716708978617362
1103	76-77	-0.05372282919385185
1103	78-79	0.10211330986530953
1103	80-81	0.03735439158280229
1103	82-83	0.06950573653652015
1103	84-85	0.10030243725415744
1103	86-87	0.07123450659032216
1103	88-89	0.09075670473183806
1103	90-91	0.050985929982026335
1103	92-93	0.02853342785838464
1103	94-95	0.0318434466041424
1103	96-97	0.007325335505441899
1103	98-99	-0.008233986236298563
1103	100	0.09286459380665235
1104	1	0.07241008721025821
1104	2	0.02821376456866176
1104	3	-9.080839855570844E-4
1104	4	0.038304468613389986
1104	5	-0.02113076806702452
1104	6	0.009845050020977908
1104	7	-0.0010068327801846522
1104	8	-0.007194682092077187
1104	9	0.017779100403622294
1104	10-11	-0.033191651449342885
1104	12-13	-0.02508401590578302
1104	14-15	0.008780743716975792
1104	16-17	-0.034760998174725444
1104	18-19	-0.03614370098262043
1104	20-21	-0.08030326779734054
1104	22-23	-0.12100546462095707
1104	24-25	-0.10257162343679482
1104	26-27	-0.04796633687990237
1104	28-29	-0.10395782306341772
1104	30-31	-0.08468579740925719
1104	32-33	-0.11898423666994518
1104	34-35	-0.13348097244431045
1104	36-37	-0.08030263179903585
1104	38-39	-0.031337587409304035
1104	40-41	-0.0844849828636356
1104	42-43	-0.05830954585524495
1104	44-45	-0.10417050955911833
1104	46-47	-0.17023340514470675
1104	48-49	-0.14872073030920063
1104	50-51	-0.12520840427719548
1104	52-53	-0.18245320804874865
1104	54-55	-0.2809906730273113
1104	56-57	-0.16755113643426256
1104	58-59	-0.09503949899756492
1104	60-61	-0.1619170851306393
1104	62-63	-0.16393219182987906
1104	64-65	-0.17442378768102174
1104	66-67	-0.1701287539641001
1104	68-69	-0.16534785575926492
1104	70-71	-0.13298642855188092
1104	72-73	-0.17294542934951096
1104	74-75	-0.16117124968992158
1104	76-77	-0.22670002617789464
1104	78-79	-0.11551974466689074
1104	80-81	-0.10551010484434897
1104	82-83	-0.0831690388122368
1104	84-85	-0.07685967641592839
1104	86-87	-0.07980623004241494
1104	88-89	-0.08364704629789088
1104	90-91	-0.0330816862161285
1104	92-93	-0.05996273457996182
1104	94-95	-0.10575790068291724
1104	96-97	-0.09951463643821512
1104	98-99	-0.11554497681650844
1104	100	-0.08471375922025004
1105	1	0.09503102714272416
1105	2	0.07426915772146714
1105	3	0.03758669987161767
1105	4	0.015645215899589004
1105	5	-0.00880065465132418
1105	6	0.01343945697365001
1105	7	0.006008564788118065
1105	8	-0.015420093244941313
1105	9	-0.04103398935876612
1105	10-11	0.024263806187306614
1105	12-13	0.003790398294256647
1105	14-15	0.031136694384642283
1105	16-17	0.018206248275589587
1105	18-19	-0.05009098815522606
1105	20-21	-0.06189864496682418
1105	22-23	-0.06075345977969704
1105	24-25	-0.03841203996420717
1105	26-27	-0.02944624327208345
1105	28-29	-0.035932835283603026
1105	30-31	-0.008467731190961558
1105	32-33	-0.036573959644101706
1105	34-35	-0.023907084777121668
1105	36-37	0.0021477570723291706
1105	38-39	0.02486594012849963
1105	40-41	0.02457129554084503
1105	42-43	0.05481517890474663
1105	44-45	0.059523499640071975
1105	46-47	0.00588607347685155
1105	48-49	-0.03307957432044617
1105	50-51	-0.03888035952750357
1105	52-53	-0.09196824894277
1105	54-55	-0.05155442509192909
1105	56-57	-0.05299914287385121
1105	58-59	-0.019345806747523397
1105	60-61	-0.04416308797990354
1105	62-63	-0.06117973907206675
1105	64-65	-0.00891136734538378
1105	66-67	-0.0144496766165787
1105	68-69	-0.008611769351816179
1105	70-71	-0.03397989645805666
1105	72-73	-0.08488375366278689
1105	74-75	-0.08924394596188279
1105	76-77	-0.06676670005726493
1105	78-79	-0.051205335187233914
1105	80-81	-0.03301739054990804
1105	82-83	-0.049231491918874326
1105	84-85	-0.008926334423172477
1105	86-87	-0.04363900369677509
1105	88-89	0.0024344145664443317
1105	90-91	0.027882350120798094
1105	92-93	-0.030418558485898473
1105	94-95	-0.049177242212415706
1105	96-97	-0.05326656407103769
1105	98-99	-0.058087182077784405
1105	100	0.045001044381123734
1106	1	0.10089684550024458
1106	2	0.06312581468078804
1106	3	0.03153079144613713
1106	4	-0.021340319891031356
1106	5	0.006082262403900529
1106	6	0.013706602126767109
1106	7	0.011483319649364887
1106	8	0.015716597313790714
1106	9	0.01477791613907442
1106	10-11	0.006110852781873177
1106	12-13	-0.03664697848157772
1106	14-15	1.987657371671503E-5
1106	16-17	0.027916870674410177
1106	18-19	-0.005370098412136315
1106	20-21	-0.02949111537096627
1106	22-23	-0.045355829058188135
1106	24-25	-0.014817981840934635
1106	26-27	-0.02291247822764575
1106	28-29	-0.009590058240235066
1106	30-31	-0.03578089836525322
1106	32-33	-0.05075976618233824
1106	34-35	0.03252369172114555
1106	36-37	0.03631707252617389
1106	38-39	-0.007179639518710701
1106	40-41	0.03878273496268747
1106	42-43	0.015862414275332526
1106	44-45	0.023316844829487593
1106	46-47	0.052570851068232116
1106	48-49	0.06260068363709337
1106	50-51	0.03492446146144346
1106	52-53	0.003978796603689716
1106	54-55	0.04585323110401873
1106	56-57	-0.025941343581841636
1106	58-59	-0.05234218312487826
1106	60-61	0.05536925938951498
1106	62-63	-0.027567877380320738
1106	64-65	0.021402003707549966
1106	66-67	0.003982439451256425
1106	68-69	-0.010787670423098916
1106	70-71	0.043767181577571534
1106	72-73	0.04365816277271506
1106	74-75	0.02522613752421421
1106	76-77	0.014243503283019265
1106	78-79	0.03300868808290858
1106	80-81	0.05132971923296026
1106	82-83	0.07021772333832388
1106	84-85	0.06954221877754208
1106	86-87	0.0926159915589011
1106	88-89	0.06272503515017291
1106	90-91	0.06099668916258594
1106	92-93	0.0013307121781096498
1106	94-95	0.01679045311356475
1106	96-97	0.0865213610261435
1106	98-99	0.06500748400518574
1106	100	0.0696178981384179
1107	1	0.09606247233695342
1107	2	0.04774094134770479
1107	3	0.02464270754753528
1107	4	0.032468953938398215
1107	5	0.004733213968080463
1107	6	0.0020815261229145676
1107	7	0.022446394061738317
1107	8	0.021194584972697328
1107	9	0.020717940210296604
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2316	7	-0.02497038808316887
2316	8	0.038094867178564584
2316	9	0.008582060405984748
2316	10-11	-0.015227797400392262
2316	12-13	-0.04876671824244028
2316	14-15	0.027253686952931844
2316	16-17	-0.010654474242791423
2316	18-19	-0.008337638180456963
2316	20-21	-0.010381749045812683
2316	22-23	0.004738100130346368
2316	24-25	-0.006562666503235448
2316	26-27	0.0010840618358045617
2316	28-29	0.01133162349010064
2316	30-31	-0.3250374002007135
2316	32-33	-0.08614280699932664
2316	34-35	-0.16763748042828297
2316	36-37	-0.44971431470490586
2316	38-39	-0.2610762420343917
2316	40-41	-0.20816092195807556
2316	42-43	-0.39760157609516966
2316	44-45	-0.19243054205238508
2316	46-47	-0.2902851312627277
2316	48-49	-0.26126231837044855
2316	50-51	-0.2758111021817413
2316	52-53	-0.3107603283010505
2316	54-55	-0.10586879853625675
2316	56-57	-0.8967868999640842
2316	58-59	-0.6715684001322018
2316	60-61	-0.3209250761106759
2316	62-63	-0.0638654442600668
2316	64-65	-0.030713316243236477
2316	66-67	-3.338969818216242E-4
2316	68-69	-0.1340593110538819
2316	70-71	-0.16544810392516496
2316	72-73	-0.06999903391216122
2316	74-75	-0.06806799117261164
2316	76-77	-0.03405169109466044
2316	78-79	-0.1150810414636183
2316	80-81	-0.1097585512580963
2316	82-83	-0.2999258160388365
2316	84-85	-0.10459685892718795
2316	86-87	-0.1881626623539674
2316	88-89	-0.1712495654168933
2316	90-91	-0.07007869405381939
2316	92-93	-0.12559633920202629
2316	94-95	-0.11634236444093204
2316	96-97	-0.3162858002504976
2316	98-99	-0.11372319161097977
2316	100	-0.3682351639021384
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	17.0
13	87.0
14	406.0
15	1222.0
16	3188.0
17	7189.0
18	13375.0
19	20827.0
20	31352.0
21	47383.0
22	61964.0
23	62390.0
24	57421.0
25	56027.0
26	62362.0
27	74488.0
28	91305.0
29	112407.0
30	138204.0
31	171485.0
32	217391.0
33	286538.0
34	432191.0
35	660051.0
36	1179485.0
37	2336299.0
38	2550916.0
39	638185.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53644494864968	20.523641013578956	13.706149506957885	23.233764530813474
2	15.583560869651109	20.18087526829817	40.5973783865263	23.63818547552442
3	18.528572528282	26.44571870786983	29.84898550472567	25.176723259122504
4	12.546469419240966	16.98640461489405	39.265378954047534	31.201747011817453
5	13.011792504994158	38.482149548383795	34.83164162912951	13.674416317492536
6	30.16253467778646	38.08019264959761	17.799721289369486	13.957551383246441
7	26.85375722288912	32.68885209247316	22.804698532248167	17.652692152389555
8	26.220611935454397	35.14415441317384	19.84961161261688	18.785622038754887
9	26.398103334228402	15.334695651044248	21.126152817432715	37.14104819729464
10-11	25.33262245614653	26.17380625132457	27.627686805490992	20.86588448703791
12-13	25.70964293722313	23.93516452552681	28.05131821563466	22.303874321615403
14-15	23.1665958525567	25.184816774865084	25.7597933498438	25.88879402273442
16-17	22.00484941961082	28.011209203300663	26.818388757815192	23.16555261927332
18-19	21.53115179676688	27.59145415133618	29.197437684059768	21.679956367837168
20-21	22.92823691224924	26.727276689898684	28.721935226271945	21.62255117158013
22-23	23.098431761766886	26.404099586383545	28.449308432067212	22.048160219782357
24-25	22.58866991900643	26.562325537452008	28.912480997002465	21.936523546539092
26-27	22.46668319350476	26.83129838139698	28.284513132340784	22.417505292757475
28-29	21.88102920043592	27.117901733424738	28.733956290425862	22.26711277571349
30-31	22.67427378630478	26.641277630198175	28.67041786799519	22.014030715501857
32-33	22.70744997955544	26.967235663898347	28.076256266514566	22.249058090031646
34-35	22.666993225374256	26.98032229669412	28.603643699396198	21.74904077853543
36-37	22.5478237933247	26.68029475190405	28.28466570361674	22.487215751154512
38-39	22.580386940518586	26.709055652831797	28.697091659274758	22.01346574737486
40-41	22.43490908903488	26.121041600607665	29.107754923138195	22.33629438721926
42-43	22.31471611521426	26.306654664286235	28.546933187082953	22.831696033416552
44-45	22.394063214349575	26.897993423696093	28.387804919553734	22.3201384424006
46-47	22.458736831571887	26.73808004806655	27.814472016331976	22.988711104029587
48-49	22.93427227022278	26.74488348974765	27.885219786438135	22.43562445359143
50-51	22.745555627251925	26.689711979930358	27.66378114421467	22.90095124860304
52-53	22.34407626373985	27.055056247815067	27.573202255925196	23.027665232519887
54-55	22.275093986526496	27.002373351041758	27.57829078999145	23.1442418724403
56-57	22.962271036276142	26.713426651462807	27.222809908588975	23.101492403672072
58-59	22.194104052473506	26.99818300150887	27.828522538876406	22.97919040714122
60-61	22.432755345133373	27.394641599378932	27.28682340324258	22.885779652245116
62-63	22.614474420138688	26.90215505165402	27.164511640063914	23.318858888143374
64-65	21.99098073937796	26.905601705079153	27.422146768261108	23.68127078728178
66-67	22.491871139190373	26.717053587969197	27.337150484215506	23.453924788624924
68-69	22.104420807933632	26.737990621492408	27.57160770711846	23.585980863455497
70-71	22.113849384134163	26.44005641037231	27.600542373565563	23.84555183192797
72-73	22.466706910495237	26.588535662357675	27.529312547190095	23.41544487995699
74-75	22.522198720151177	26.913492097577734	27.307249613468475	23.25705956880261
76-77	22.223760534118593	26.826941468461087	27.07969643207136	23.869601565348965
78-79	22.16891572304572	26.741177188659577	27.181168296827018	23.908738791467684
80-81	21.94825645175871	27.19640655071427	27.341066117603052	23.51427087992397
82-83	22.527274586583378	26.46638328527552	27.988284683689415	23.018057444451692
84-85	22.63130528459208	26.708841379782744	27.160469438515918	23.49938389710926
86-87	21.651985456773993	27.239806742392638	27.52485951223707	23.583348288596294
88-89	22.23828913616798	26.342057028794404	27.48836396836992	23.931289866667697
90-91	22.132369969068	26.39408445269603	27.500758779266683	23.972786798969285
92-93	22.320434977683913	26.54643754818618	27.32265579630439	23.810471677825515
94-95	21.486313548819812	26.424551486172366	27.849179470003822	24.239955495004008
96-97	21.721335852226463	26.407144875578474	27.58907850254409	24.28244076965097
98-99	21.259758234822062	26.737897582493165	27.50766430389214	24.494679878792635
100	21.69315804922817	26.333585925335978	27.074151463727354	24.899104561708498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1283.0
1	869.5
2	663.0
3	873.5
4	729.0
5	714.5
6	1203.0
7	2261.0
8	3643.0
9	4531.5
10	4163.0
11	3551.5
12	3844.5
13	4600.5
14	5364.5
15	6289.5
16	7339.5
17	8418.0
18	9474.5
19	10387.5
20	11627.5
21	13082.0
22	14501.5
23	16562.0
24	19367.5
25	22847.5
26	28110.0
27	34857.5
28	43895.0
29	58828.5
30	78906.0
31	99356.5
32	127003.5
33	157651.0
34	184177.0
35	211346.0
36	240131.5
37	278775.5
38	298457.0
39	295179.5
40	287093.5
41	274602.5
42	273715.0
43	286826.5
44	306880.5
45	344019.0
46	375586.5
47	395192.0
48	412410.5
49	409486.0
50	396145.0
51	394966.0
52	392493.0
53	374111.0
54	356792.0
55	319266.5
56	280211.5
57	236236.5
58	190373.5
59	172516.5
60	141921.5
61	104230.5
62	80106.5
63	64311.0
64	46121.0
65	26171.5
66	16816.5
67	11937.5
68	8656.0
69	6294.5
70	4378.0
71	3223.0
72	2366.5
73	1622.0
74	1121.0
75	738.5
76	479.5
77	278.0
78	157.5
79	87.0
80	55.0
81	39.0
82	21.5
83	11.0
84	8.0
85	5.0
86	5.5
87	3.0
88	2.0
89	3.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35715972824639913
2	6.012247807676782E-4
3	0.001943244380695531
4	0.0013957003839249673
5	0.004702436678147197
6	0.0
7	7.515309759595977E-5
8	3.9723780157864454E-4
9	0.003124221628632042
10-11	6.441694079653695E-5
12-13	5.9048862396825536E-5
14-15	2.9524431198412767E-4
16-17	1.0199348959451684E-4
18-19	9.662541119480542E-4
20-21	2.0398697918903367E-4
22-23	1.5567427359163095E-4
24-25	0.0
26-27	0.00190029975349784
28-29	0.0112890688745931
30-31	0.0034999871166118404
32-33	0.01425761622963351
34-35	0.011144130757800892
36-37	0.012878020080907677
38-39	0.012980013570502194
40-41	0.01403215693684563
42-43	0.006570527961246768
44-45	0.001304443051129873
46-47	0.006076664748473318
48-49	0.013248417490487765
50-51	0.003768391036597411
52-53	0.005754580044490634
54-55	0.005207036047720069
56-57	1.82514665590188E-4
58-59	6.226970943665238E-4
60-61	3.167166255829733E-4
62-63	9.5014987674892E-4
64-65	3.3282086078210754E-4
66-67	0.0014010684623246786
68-69	0.0016265277551125578
70-71	8.105798383564232E-4
72-73	0.0028182411598484916
74-75	0.007730032895584433
76-77	0.0038489122125930824
78-79	0.0016587362255108264
80-81	4.0797395837806734E-4
82-83	1.8788274398989942E-4
84-85	0.0013849642271255443
86-87	0.004621915502151526
88-89	0.0015889512063145782
90-91	1.82514665590188E-4
92-93	0.002007661321492068
94-95	0.0033228405294213645
96-97	0.0018895635966984172
98-99	0.0021418632814848536
100	0.002383426809471867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9314320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.49259810141163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0947105521785	38.19642891154639
2	15.880825065834742	17.30774835662713
3	6.086933615285914	9.950784815032433
4	3.033992279154963	6.613204876431092
5	1.5836551242398114	4.314874110822057
6	0.9376822691213876	3.0658045822830884
7	0.6117637271397277	2.333561644123278
8	0.39150973249167975	1.7067506004368225
9	0.27723524388980497	1.3596541852350577
>10	1.039518898000557	9.174772411602943
>50	0.037516657497375594	1.397488107091939
>100	0.022126759172425802	2.2964238474650185
>500	0.0016446433634179317	0.59900877281999
>1k	7.589422601821548E-4	0.6595572058568107
>5k	5.059614782859819E-5	0.16421380210459918
>10k+	7.589422174289727E-5	0.8597237705214258
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26589	0.2854636731398535	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22774	0.2445052349500554	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13001	0.13958077454929613	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.147231359884565E-5
7	0.0	0.0	0.0	0.0	2.147231359884565E-5
8	0.0	0.0	0.0	0.0	2.147231359884565E-5
9	0.0	0.0	0.0	0.0	2.147231359884565E-5
10-11	0.0	0.0	0.0	0.0	2.147231359884565E-5
12-13	0.0	0.0	0.0	0.0	1.0736156799422825E-4
14-15	0.0	0.0	0.0	0.0	1.1809772479365107E-4
16-17	0.0	0.0	0.0	5.368078399711412E-6	1.3957003839249672E-4
18-19	0.0	0.0	0.0	1.0736156799422825E-5	1.3957003839249672E-4
20-21	0.0	0.0	0.0	4.29446271976913E-5	1.6104235199134237E-4
22-23	0.0	0.0	0.0	3.167166255829733E-4	1.6104235199134237E-4
24-25	0.0	0.0	0.0	7.139544271616179E-4	1.6104235199134237E-4
26-27	0.0	0.0	0.0	0.0016748404607099606	1.6641043039105377E-4
28-29	0.0	0.0	0.0	0.004890319422137097	1.8251466559018802E-4
30-31	0.0	0.0	0.0	0.01736573362306642	1.8251466559018802E-4
32-33	0.0	0.0	0.0	0.045333422085562874	1.8251466559018802E-4
34-35	0.0	0.0	0.0	0.09054337836793239	1.8251466559018802E-4
36-37	0.0	0.0	0.0	0.16207839112248668	1.8251466559018802E-4
38-39	5.368078399711412E-6	0.0	0.0	0.26565009576651866	1.8251466559018802E-4
40-41	1.0736156799422825E-5	0.0	0.0	0.3765384912693573	1.8251466559018802E-4
42-43	1.0736156799422825E-5	0.0	0.0	0.484839472983535	1.8788274398989942E-4
44-45	1.0736156799422825E-5	0.0	0.0	0.6158313220932929	1.9325082238961085E-4
46-47	1.0736156799422825E-5	0.0	0.0	0.7722141820336857	2.0935505758874507E-4
48-49	1.0736156799422825E-5	0.0	0.0	0.9424735246373326	2.4693160638672494E-4
50-51	1.0736156799422825E-5	0.0	0.0	1.122336359498063	2.4693160638672494E-4
52-53	1.0736156799422825E-5	0.0	0.0	1.2954193113399581	2.6303584158585917E-4
54-55	1.0736156799422825E-5	0.0	0.0	1.4858035798641231	2.684039199855706E-4
56-57	1.0736156799422825E-5	0.0	0.0	1.7056961753515019	2.8450815518470487E-4
58-59	1.0736156799422825E-5	0.0	0.0	1.9548662704309065	3.006123903838391E-4
60-61	1.0736156799422825E-5	0.0	0.0	2.215642151010487	3.006123903838391E-4
62-63	1.0736156799422825E-5	0.0	0.0	2.476874318254043	3.006123903838391E-4
64-65	1.0736156799422825E-5	0.0	0.0	2.742502941706963	3.0598046878355047E-4
66-67	1.0736156799422825E-5	0.0	0.0	3.037124556596724	3.113485471832619E-4
68-69	1.0736156799422825E-5	0.0	0.0	3.36142090888009	3.113485471832619E-4
70-71	1.0736156799422825E-5	0.0	0.0	3.7144311125235125	3.220847039826847E-4
72-73	1.0736156799422825E-5	0.0	0.0	4.073405251269014	3.6502933118037604E-4
74-75	1.0736156799422825E-5	0.0	0.0	4.429754399677057	3.6502933118037604E-4
76-77	1.0736156799422825E-5	0.0	0.0	4.8170880966082334	3.6502933118037604E-4
78-79	1.0736156799422825E-5	0.0	0.0	5.23456892183219	3.6502933118037604E-4
80-81	1.0736156799422825E-5	0.0	0.0	5.686872471635073	3.6502933118037604E-4
82-83	1.0736156799422825E-5	0.0	0.0	6.150556347645346	3.7576548797979884E-4
84-85	1.0736156799422825E-5	0.0	0.0	6.6027471678018355	3.865016447792217E-4
86-87	1.0736156799422825E-5	0.0	0.0	7.071133480490256	3.865016447792217E-4
88	1.0736156799422825E-5	0.0	0.0	7.444526277817382	3.865016447792217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17300	0.0	49.163826	1
GTATCAA	30610	0.0	40.00713	1
CAACGCA	35340	0.0	33.352898	5
ATCAACG	35820	0.0	32.907017	3
TATCAAC	38925	0.0	31.343922	2
AACGCAG	39465	0.0	30.592522	6
TCAACGC	40460	0.0	29.47007	4
GTGGTAT	6140	0.0	29.272017	1
ACGCAGA	42630	0.0	27.615824	7
CGCAGAG	43030	0.0	27.216984	8
TAGGCAT	8755	0.0	26.142399	5
GGGCACC	8545	0.0	25.739874	94
CTGTGCG	9740	0.0	25.041885	9
TAGTACT	10090	0.0	24.64059	4
GTACATG	41215	0.0	24.631054	1
GCAGAGT	47075	0.0	24.49869	9
TACATGG	41715	0.0	23.997425	2
CTAGGCA	10045	0.0	23.955576	4
CTAACGC	3260	0.0	23.643559	3
ACATGGG	41245	0.0	23.416775	3
>>END_MODULE