FastQCFastQC Report
Thu 26 May 2016
ERR580205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4355237
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74010.16993334691085699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61920.1421736635687105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68850.048.837921
GTATCAA120550.040.766571
CAACGCA139600.034.0890775
ATCAACG141250.033.727653
TATCAAC152750.032.01972
AACGCAG155550.031.1227326
TCAACGC159250.030.0634214
GTGGTAT22950.029.9174981
ACGCAGA174600.027.35087
GTACTAG25700.026.8991951
CGCAGAG181200.026.1995418
GCAGAGT197400.023.2869289
GCATCGT26100.023.231638
GTACATG194000.023.1018241
TACATGG197800.022.1354562
ACATGGG200500.021.227523
TAGGCAT37700.021.2044855
CTAGGCA40200.020.4730264
TGGTATC32250.020.269832
CAGAGTA192000.019.77951810-11
Produced by FastQC (version 0.11.5)