FastQCFastQC Report
Thu 26 May 2016
ERR580205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4355237
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64880.14897007901062562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.12502189892306664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68750.048.0669631
GTATCAA121850.040.159591
CAACGCA143750.032.5976685
ATCAACG148300.031.7884243
TATCAAC155650.031.2841842
GTGGTAT25300.029.7278521
AACGCAG162750.029.3391176
TCAACGC165200.028.5358034
ACGCAGA180000.026.0052577
CGCAGAG184050.025.35678
GCAGAGT203700.022.541769
GTACATG195400.022.2045121
GTACTAG22900.021.7587761
TACATGG199250.021.631432
TACCCCG3950.021.417795
ACATGGG200300.020.790363
TGGTATC34850.020.769792
GGGCTAT34600.020.646286
GAGTACT78600.020.33003612-13
TATTCTC22600.020.172635
Produced by FastQC (version 0.11.5)