FastQCFastQC Report
Thu 26 May 2016
ERR580204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5202927
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75690.14547580621446352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62790.12068206991948954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85500.045.3096771
GTATCAA156700.040.9237331
CAACGCA165000.036.780525
ATCAACG173250.035.058563
TATCAAC188500.033.845952
TACCCCG5200.032.544285
AACGCAG197050.032.0988666
CGTTAAC15200.031.2397581
GTACTAG52050.030.349281
GTGGTAT23800.029.6308561
GCATCGT49850.029.1262868
ACGCAGA208400.028.861077
CGCAGAG210500.028.416348
TCAACGC226850.027.1479664
TAGGCAT69250.027.0171075
GCAGAGT230900.025.4837959
GGGCACC45850.025.31650294
AAGCTAA44300.024.1888289
CTAGGCA79500.023.7127574
GTACATG271450.022.8966261
Produced by FastQC (version 0.11.5)