FastQCFastQC Report
Thu 26 May 2016
ERR580204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5202927
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65750.12637117530190217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57900.11128351406813894No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86900.045.8090441
GTATCAA151450.039.395791
CAACGCA164550.034.3009155
ATCAACG171300.032.9228173
TATCAAC187450.031.7153722
GTACTAG46950.031.5444261
AACGCAG202150.029.17526
TAGGCAT64150.028.0583865
GTGGTAT25000.027.9222411
CGTTAAC16950.027.5482561
ACGCAGA210950.026.6440147
GGGCACC47700.026.50372994
CGCAGAG214000.026.0885948
CTAGGCA72500.025.410724
TCAACGC223100.025.3837854
ACGTTAA13650.024.7883648
GCAGAGT229750.023.9935599
TACCCCG4100.022.9248585
GTACATG267650.022.6798341
TACATGG266650.022.3834422
Produced by FastQC (version 0.11.5)