FastQCFastQC Report
Thu 26 May 2016
ERR580203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6421295
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165670.25800091726045915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140800.2192704119651877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79220.12337075309575404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105700.051.1219141
GTATCAA181700.039.600311
GTACTAG43650.035.5542681
CAACGCA208350.033.3446585
ATCAACG214600.032.285413
TATCAAC230300.030.9686032
AACGCAG234100.030.3864696
TAGGCAT58100.028.801775
TCAACGC244500.028.6074774
GTGGTAT29650.028.3916361
ACGCAGA253550.027.2789127
CTAGGCA62900.027.0528764
CGCAGAG258700.026.6262658
GCATCGT52600.026.6197038
GTACATG244100.026.529451
TACATGG247350.025.5836892
ACATGGG247700.024.6312943
GCAGAGT280350.023.9518039
TAGTACT64600.023.648654
GGGCACC56450.023.64699794
Produced by FastQC (version 0.11.5)