FastQCFastQC Report
Thu 26 May 2016
ERR580203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6421295
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148690.23155765309022555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130130.20265382605845084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76810.11961761607277037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105550.048.3307881
GTATCAA180950.039.2087551
GTACTAG44000.032.0568581
CAACGCA212700.031.951225
ATCAACG217050.031.4198723
GTGGTAT30750.031.0386831
TATCAAC232400.030.4452082
AACGCAG237400.029.4372066
TCAACGC245500.027.8548954
TAGGCAT61100.026.6916165
ACGCAGA256250.026.4095867
CTGTGCG62000.025.6961219
CGCAGAG264100.025.4820388
TAGTACT62800.025.295724
GTACATG245600.025.2312891
TCTAACG21650.024.7551462
TACATGG247150.024.72872
CTAACGC21350.024.6556783
TAACGCC22550.024.385374
CTAGGCA66800.024.13284
Produced by FastQC (version 0.11.5)